2011 Fiscal Year Final Research Report

Analysis of nonalcoholic fatty liver using androgen receptor transgenic mouse

Research Project

Project/Area Number	21590828
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Gastroenterology
Research Institution	Chiba University
Principal Investigator	IMAZEKI Fumio 千葉大学, 大学院・医学研究院腫瘍内科学, 准教授 (40223325)
Co-Investigator(Renkei-kenkyūsha)	ARAI Makoto 千葉大学, 医学部・附属病院, 助教 (30396684)
Project Period (FY)	2009 – 2011
Keywords	アンドロジェン受容体トランスジェニックマウス
Research Abstract	We had planned to examine the relationship between androgen receptor and lipid or carbohydrate metabolism using androgen receptor transgenic mouse, but the transgenic mouse could not be produced for examination. Two female transgenic mice produced in the first experiment did not raise their children. A total of 139 mice produced in the next 3 consecutive experiments did not have the transgenic genes. Therefore we transfected androgen receptor expression vector into HepG2 and Huh7, and analyzed the change of m-RNA expression in the 84 genes related to lipid metabolism. Among these genes, the only genes down regulated both in the two cells were Acyl-CoA dehydrogenase and Carnitine palmitoyltransferase 1A, both of which are involved in fatty acid oxidation and the result was contrary to our expectation.