2011 Fiscal Year Final Research Report
Prospective analysis of DNA damage and repair markers in the patients with high risk of hepatocellular cancer
| Project/Area Number |
21590835
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 肝臓病学 |
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| Research Abstract |
Background : It has been widely considered that the DNA damage response is strongly involved in the step of early carcinogenesis. In case of hepatocellular carcinoma(HCC), however, it has been remained unclear whether DNA damage response molecules are useful for the tumor markers of the patients with high risk of HCC.
Methods : We evaluated the phosphorylation levels of DNA damage response markers in animal models with HCC by Western blotting or immunohistochemical staining. Based on the animal model experiments, we addressed whether any of the DNA-damage response molecules are involved in the cancer development of the patients with high risk of HCC.
Results : Western blotting and immunohistochemical analysis showed that the levels of gamma-H2A were significantly increased in HCC-prone mice. Interestingly, increase in the levels of gamma-H2AX was observed from several months before the development of HCC. The increase in the levels of gamma-H2AX and Wip1 activity was also observed in human liver tissues adjacent to HCC.
Conclusion : DNA damage response molecules gamma-H2AX and Wip1 might be useful markers for predicting the development of HCC.
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Author(s)
Kawai H, Nomoto M, Suda T, Kamimura K, Tsuchiya A, Tamura Y, Yano M, Takamura M, Igarashi M, Wakai T, Yamagiwa S, Matsuda Y, Ohkoshi S, Kurosaki I, Shirai Y, Okada M, Aoyagi Y
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Journal Title
World J Hepatol
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Pages: 15-23
Peer Reviewed
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Author(s)
Aoki YH, Ohkoshi S, Yamagiwa S, Yano M, Takahashi H, Waguri N, Igarashi K, Sugitani S, Takahashi T, Ishikawa T, Kamimura T, Wakabayashi H, Watanabe T, Matsuda Y, Nomoto M, Aoyagi Y
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Journal Title
Hepatol Res
Volume: 41(2)
Pages: 118-125
Peer Reviewed
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[Journal Article] MRP2 determines the efficacy of cisplatin in patients with hepatocellular carcinoma2010
Author(s)
Korita PV, Wakai T, Shirai Y, Matsuda Y, Sakata J, Takamura M, Yano M, Sanpei A, Aoyagi Y, Hatakeyama K, Ajioka Y
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Journal Title
Oncol Rep
Volume: 23(4)
Pages: 965-972
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[Journal Article] Loss of liver-intestine cadherin in human intrahepatic cholangiocarcinoma promotes angiogenesis by upregulating metal-responsive transcription factor-1 and placental growth factor2010
Author(s)
Takamura M, Yamagiwa S, Wakai T, Tamura Y, Kamimura H, Kato T, Tsuchiya A, Matsuda Y, Shirai Y, Ichida T, Ajioka Y, Aoyagi Y
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Journal Title
Int J Oncol
Volume: 36(1)
Pages: 245-254
Peer Reviewed
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[Journal Article] Progression of hypermethylation of the p16(INK4A) gene from normal liver to nontumorous liver and hepatocellular carcinoma : an evaluation using quantitative PCR analysis2009
Author(s)
Kurita S, Ohkoshi S, Yano M, Yamazaki K, Suzuki K, Aoki YH, Matsuda Y, Wakai T, Shirai Y, Ichida T, Aoyagi Y
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Journal Title
Dig Dis Sci
Volume: 54(1)
Pages: 80-88
Peer Reviewed
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[Journal Article] Reduced NKG2D ligand expression in hepatocellular carcinoma correlates with early recurrence
Author(s)
Kamimura H, Yamagiwa S, Tsuchiya A, Takamura M, Matsuda Y, Ohkoshi S, Inoue M, Wakai T, Shirai Y, Nomoto M, Aoyagi Y
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Journal Title
J Hepatol
Volume: (in press)
Peer Reviewed
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