2011 Fiscal Year Final Research Report
Prediction of tumor dynamics using amountof serum methylated DNA in human hepatocellular carcinoma
| Project/Area Number |
21590840
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kinki University (2011)
Kyoto University (2009-2010) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIMURA Takafumi 京都大学, 大学院・医学研究科, 講師 (40378732)
FUKUDA Yoshihiro 京都大学, 大学院・医学研究科, 教授 (50127130)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 肝臓学 / 肝細胞癌 / 診断 / 腫瘍マーカー / エピジェネティクス / 癌抑制遺伝子 |
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| Research Abstract |
We applied tumor specific methylated DNA for prediction of tumor dynamics in HCC cases. The methylated APC and GSTP1 genes were identified as useful marker for discrimination of HCC cells from non-cancerous liver. We established a sensitive assay for quantifying of methylated/non-methylated(Me/NMe) DNA ratio from serum of 100μl. We successfullydetect Me-DNA from all HCC cases examined and no normal control cases showed Me-DNA. The Me/NMe DNA ratio elevated quicker than conventional HCC marker, AFP, when tumor showed recurrence. This novel assay could be a promising approachfor judgmentof theraupic effectof HCCincluding dormant therapy.
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[Journal Article] Analysis of fecal DNA methylation for detecting gastrointestinal neoplasia2009
Author(s)
Nagasaka T, Tanaka N, Cullings H, Sun D, Sasamoto H, Uchida T, Koi M, Nishida N, Boland CR, Matsubara N, and Goel A
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Journal Title
J Natl Cancer Inst
Volume: 101
Pages: 1224-1258
URL
Peer Reviewed
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