2011 Fiscal Year Final Research Report

Controlling HCV replication by targeting intracellular signaling

Research Project

Project/Area Number	21590843
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Gastroenterology
Research Institution	独立行政法人国立病院機構大阪医療センター(臨床研究センター) (2011) Osaka University (2009-2010)
Principal Investigator	ISHIDA Hisashi 独立行政法人国立病院機構大阪医療センター(臨床研究センター), 研究員 (00506230)
Co-Investigator(Kenkyū-buntansha)	TAKEHARA Tetsuo 大阪大学, 大学院・医学系・研究科, 教授 (70335355)
Project Period (FY)	2009 – 2011
Research Abstract	In this study, we investigated factors affecting intracellular signaling which could control HCV replication. Among microRNAs which were regulated by HCV infection, miR192/miR-215 and miR-491 were identified as the factors which could regulate HCV replication. miR-491 was demonstrated to suppress PI3 kinase/Akt pathway, responsible for HCV augmentation. Branched-chain amino acids could suppress HCV RNA replication whereas enhance HCV particle formation, which suggested some signaling regulated by BCAA may be involved in controlling HCV replication.