Loss of HB-EGF accelerates carbon tetrachloride induced liver injury in mice

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590844
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Osaka University
• Principal Investigator: KISO Shinichi 大阪大学, 医学系・研究科, 助教 (40335352)
• Co-Investigator(Kenkyū-buntansha): YOSHIA Yuichi 大阪大学, 医学系・研究科, 助教 (30457014) ; WATABE Kenji 大阪大学, 医学系・研究科, 助教 (50379244) ; TSUTSUI Shusaku 大阪大学, 医学系・研究科, 助教 (10359846)
• Project Period (FY): 2009 – 2011
• Keywords: HB-EGF / 肝再生 / 肝線維化

Research Abstract

Heparin-binding EGF-like growth factor(HB-EGF) is a member of EGF family of growth factors that binds to and stimulates EGF receptors(EGFR) and EerbB4. Previously, we demonstrated that the HB-EGF was induced in response to several liver injuries and also showed that transgenic expression of HB-EGF in mice accelerated proliferation of hepaocyte during liver regeneration after partial hepatectomy. However, the role of HB-EGF in acute liver injury induced by hepatotoxin remains still unclear. To clarify this issue, we generated liver specific HB-EGF knockout(KO) mice. At 24 hours after CCl4 injection, KO mice showed significant increased ALT level compared with WT mice(5486+ 1961 IU/ l vs. 2275+ 656 IU/ l, p<0. 05), indicating that hepatic HB-EGF deficiency caused acceleration of liver injury. Western blotting also showed significant reduced expression level of PCNA protein at 48 hours after CCl4injection. Consistent with this, the number of BrdU positive hepatocytes in KO mice was lower than that in WT mice at 48 and 72 hours after CCl4 injection. This impaired liver regeneration in KO mice was associated with reduced activity of ERK1/ 2 MAP kinase in injured livers. Furthermore, HB-EGF significantly suppressed apoptosis of mouse hepaocyte cell lines induced by TNFαin a dose dependent manner, indicating the possible anti-apoptotic role of HB-EGF. Conclusion : Our current study demonstrated that HB-EGF might play a protective role during acute liver injury through ERK1/ 2 activation.

Research Products

• [Journal Article] Glycobiology. N-Acetylglucosaminyltransferase V regulates TGF-βresponse in hepatic stellate cells and the progression of steatohepatitis (2012)
  • Author(s): Kamada Y, Mori K, Matsumoto H, Kiso S, Yoshida Y, Shinzaki S, Hiramatsu N, Ishii M, Moriwaki K, Kawada N, Takehara T, Miyoshi E
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: (In press)
  • Peer Reviewed
• [Journal Article] Long-term effect of lamivudine treatment on the incidence of hepatocellular carcinoma in patients with hepatitis B virus infection (2012)
  • Author(s): Kurokawa M, Hiramatsu N, Oze T, Yakushijin T, Miyazaki M, Hosui A, Miyagi T, Yoshida Y, Ishida H, Tatsumi T, Kiso S, Kanto T, Kasahara A, Iio S, Doi Y, Yamada A, Oshita M, Kaneko A, Mochizuki K, Hagiwara H, Mita E, Ito T, Inui Y, Katayama K, Yoshihara H,
  • Journal Title: J Gastroenterol Volume: (In press)
  • Peer Reviewed
• [Journal Article] Protective role of adiponectin against ethanol-induced gastric injury in mice (2012)
  • Author(s): Shunsuke Yamamoto, Kenji Watabe, Hiroshi Araki, Yoshihiro Kamada, Motohiko Kato, Takashi Kizu, Shinichi Kiso, Shusaku Tsutsui, Masahiko Tsujii, Shinji Kihara, Tohru Funahashi, Iichiro Shimomura, Norio Hayashi, and Tetsuo Takehara
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: (In press)
  • Peer Reviewed
• [Journal Article] Reducing Peg-IFN doses causes later virologic response or no response in HCV genotype 1 patients treated with Peg-IFN alfa-2b plus ribavirin (2012)
  • Author(s): Oze T, Hiramatsu N, Song C, Yakushijin T, Iio S, Doi Y, Oshita M, Hagiwara H, Mita E, Ito T, Inui Y, Hijioka T, Tamura S, Yoshihara H, Inoue A, Imai Y, Hayashi E, Kato M, Miyazaki M, Hosui A, Miyagi T, Yoshida Y, Tatsumi T, Kiso S, Kanto T, Kasahara A, Hayashi N, Takehara T
  • Journal Title: J Gastroenterol Volume: 47(3) Pages: 334-42
  • Peer Reviewed
• [Journal Article] Indications and limitations for aged patients with chronic hepatitis C in pegylated interferon alfa-2b plus ribavirin combination therapy (2011)
  • Author(s): Oze T, Hiramatsu N, Yakushijin T, Mochizuki K, Oshita M, Hagiwara H, Mita E, Ito T, Fukui H, Inui Y, Hijioka T, Inada M, Kaytayama K, Tamura S, Yoshihara H, Inoue A, Imai Y, Kato M, Miyagi T, Yoshida Y, Tatsumi T, Kiso S, Kanto T, Kasahara A, Takehara T, Hayashi N
  • Journal Title: J Hepatol Volume: 54(4) Pages: 604-11
  • Peer Reviewed
• [Journal Article] Efficacy of re-treatment with pegylated interferon plus ribavirin combination therapy for patients with chronic hepatitis C in Japan (2011)
  • Author(s): Tsugiko Oze, Naoki Hiramatsu, Takayuki Yakushijin, Kiyoshi Mochizuki, Masahide Oshita, Hideki Hagiwara, Eiji Mita, Toshifumi Ito, Yoshiaki Inui, Hiroyuki Fukui, Taizo Hijioka, Kazuhiro Katayama, Shinji Tamura, Harumasa Yoshihara, Atsuo Inoue, Yasuharu Imai, Eijiro Hayashi, Michio Kato, Atsushi Hosui, Takuya Miyagi, Hisashi Ishida, Yuichi Yoshida, Tomohide Tatsumi, Shinichi Kiso, Tatsuya Kanto, Akinori Kasahara, Tetsuo Takehara and Norio Hayashi
  • Journal Title: Gastroenterol Volume: 46(8) Pages: 1031-7
  • Peer Reviewed
• [Journal Article] The efficacy of extended treatment with pegylated interferon plus ribavirin in patients with HCV genotype 1 and slow virologic response in Japan (2011)
  • Author(s): Tsugiko Oze, Naoki Hiramatsu, Takayuki Yakushijin, Kiyoshi Mochizuki, Kazuho Imanaka, Akira Yamada, Masahide Oshita, Akira Kaneko, Hideki Hagiwara, Eiji Mita, Toshifumi Ito, Toshihiko Nagase, Yoshiaki Inui, Taizo Hijioka, Shinji Tamura, Harumasa , Eijiro Hayashi, Yasuharu Imai, Michio Kato, Atsushi Hosui, Takuya Miyagi, Yuichi Yoshida, Hisashi Ishida, Tomohide Tatsumi, Shinichi Kiso, Tatsuya Kanto, Akinori Kasahara, Tetsuo Takehara, and Norio Hayashi
  • Journal Title: J Gastroenterol Volume: 46(7) Pages: 944-52
  • Peer Reviewed
• [Journal Article] Lower serum level of adiponectin is associated with increased risk of endoscopic erosive gastritis (2011)
  • Author(s): Yamamoto S, Watabe K, Tsutsui S, Kiso S, Hamasaki T, Kato M, Kamada Y, Yoshida Y, Kihara S, Umeda M, Furubayashi A, Kinoshita K, Kishida O, Fujimoto T, Yamada A, Tsukamoto Y, Hayashi N, Matsuzawa Y
  • Journal Title: Dig Dis Sci Volume: 56(8) Pages: 2354-60
  • Peer Reviewed
• [Journal Article] Association of low serum adiponectin levels with erosive esophagitis in men ; an analysis of 2405 subjects undergoing physical check-ups (2011)
  • Author(s): Motohiko Kato, Kenji Watabe, Toshimitsu Hamasaki, Miyuki Umeda, Aiko Furubayashi, Kazuo Kinoshita, Osamu Kishida, Takashi Fujimoto, Akira Yamada, Yoshifumi Tsukamoto, Shunsuke Yamamoto, Yoshihiro Kamada, Yuichi Yoshida, Shinichi Kiso, Shusaku Tsutsui, Shinji Kihara, Norio Hayashi, and Yuji Matsuzawa
  • Journal Title: J Gastroenterology Volume: 46(12) Pages: 1361-7
  • Peer Reviewed
• [Journal Article] Estrogen deficiency worsens steatohepatitis in mice fed high fat and high cholesterol diet (2011)
  • Author(s): Kamada Y, Kiso S, Yoshida Y, Chatani N, Kizu T, Hamano M, Tsubakio M, Takemura T, Ezaki H, Hayashi N, Takehara T
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 301(6) Pages: G1031-43
  • Peer Reviewed
• [Journal Article] Efficacy of pegylated interferon plus ribavirin combination therapy for hepatitis C patients with normal ALT levels : a matched case-control study (2011)
  • Author(s): Hiramatsu N, Inoue Y, Oze T, Kurashige N, Yakushijin T, Mochizuki K, Miyagi T, Tatsumi T, Kiso S, Kanto T, Kasahara A, Takehara T, Oshita M, Mita E, Hagiwara H, Inui Y, Katayama K, Tamura S, Yoshihara H, Imai Y, Hayashi N
  • Journal Title: J Gastroenterol Volume: 46(11) Pages: 1335-43
  • Peer Reviewed
• [Journal Article] Amino acid substitution in the core protein has no impact on relapse in hepatitis C genotype 1 patients treated with peginterferon and ribavirin (2011)
  • Author(s): Inoue Y, Hiramatsu N, Oze T, Yakushijin T, Mochizuki K, Fukuda K, Mita E, Haruna Y, Inoue A, Imai Y, Hosui A, Miyagi T, Yoshida Y, Tatsumi T, Kiso S, Kanto T, Kasahara A, Takehara T, Hayashi N
  • Journal Title: J Med Virol Volume: 83(3) Pages: 419-27
  • Peer Reviewed
• [Journal Article] Factors affecting efficacy in patients with genotype 2 chronic hepatitis C treated by pegylated interferon alpha-2b and ribavirin : reducing drug doses has no impact on rapid and sustained virological responses (2010)
  • Author(s): Inoue Y, Hiramatsu N, Oze T, Yakushijin T, Mochizuki K, Hagiwara H, Oshita M, Mita E, Fukui H, Inada M, Tamura S, Yoshihara H, Hayashi E, Inoue A, Imai Y, Kato M, Miyagi T, Hohsui A, Ishida H, Kiso S, Kanto T, Kasahara A, Takehara T, Hayashi N
  • Journal Title: J Viral Hepat Volume: 17(5) Pages: 336-44
  • Peer Reviewed
• [Journal Article] Adiponectin deficiency enhanced the severity of cerulein-induced chronic pancreatitis in mice (2010)
  • Author(s): Yamada T, Araki H, Watabe K, Kamada Y, Kiso S, Ogiyama H, Nishihara T, Kihara S, Funahashi T, Shimomura I, Tsutsui S, Hayashi N
  • Journal Title: J Gastroenterol Volume: 45(7) Pages: 742-9
  • Peer Reviewed
• [Journal Article] Lack of adiponectin promotes formation of cholesterol gallstones in mice (2010)
  • Author(s): Ogiyama H, Kamada Y, Kiso S, Araki H, Yamada T, Nishihara T, Watabe K, Tochino Y, Kihara S, Funahashi T, Kanno K, Shimomura I, Tsutsui S, Tazuma S, Hayashi N
  • Journal Title: Biochem Biophys Res Commun Volume: 399(3) Pages: 352-8
  • Peer Reviewed
• [Journal Article] Reduced risk of hepatocellular carcinoma after interferon therapy in aged patients with chronic hepatitis C is limited to sustained virological responders (2010)
  • Author(s): Imai Y, Tamura S, Tanaka H, Hiramatsu N, Kiso S, Doi Y, Inada M, Nagase T, Kitada T, Imanaka K, Fukuda K, Takehara T, Kasahara A, Hayashi N
  • Journal Title: J Viral Hepat Volume: 17(3) Pages: 185-91
  • Peer Reviewed
• [Journal Article] Werner syndrome as a possible cause of non-alcoholic steatohepatitis (2009)
  • Author(s): Hashizume H, Sato K, Takagi H, Kanda D, Kashihara T, Kiso S, Mori M
  • Journal Title: J Clin Pathol Volume: 62(11) Pages: 1043-5
  • Peer Reviewed
• [Journal Article] Delayed liver regeneration after partial hepatectomy in adiponectin knockout mice (2009)
  • Author(s): Ezaki H, Yoshida Y, Saji Y, Takemura T, Fukushima J, Matsumoto H, Kamada Y, Wada A, Igura T, Kihara S, Funahashi T, Shimomura I, Tamura S, Kiso S, Hayashi N
  • Journal Title: Biochem Biophys Res Commun Volume: 378(1) Pages: 68-72
  • Peer Reviewed
• [Journal Article] Transplantation of basic fibroblast growth factor-pretreated adipose tissue-derived stromal cells enhances regression of liver fibrosis in mice (2009)
  • Author(s): Kamada Y, Yoshida Y, Saji Y, Fukushima J, Tamura S, Kiso S, Hayashi N
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 296(2) Pages: G157-67
  • Peer Reviewed
• [Journal Article] Effect of interferon alpha-2b plus ribavirin therapy on incidence of hepatocellular carcinoma in patients with chronic hepatitis (2009)
  • Author(s): Kurokawa M, Hiramatsu N, Oze T, Mochizuki K, Yakushijin T, Kurashige N, Inoue Y, Igura T, Imanaka K, Yamada A, Oshita M, Hagiwara H, Mita E, Ito T, Inui Y, Hijioka T, Yoshihara H, Inoue A, Imai Y, Kato M, Kiso S, Kanto T, Takehara T, Kasahara A, Hayashi N
  • Journal Title: Hepatol Res. Volume: 39(5) Pages: 432-8
  • Peer Reviewed
• [Journal Article] Factors contributing to antiviral effect of adefovir dipivoxil therapy added to ongoing lamivudine treatment in patients with lamivudine-resistant chronic hepatitis B (2009)
  • Author(s): Kurashige N, Hiramatsu N, Ohkawa K, Yakushijin T, Kiso S, Kanto T, Takehara T, Kasahara A, Doi Y, Yamada A, Oshita M, Mita E, Hagiwara H, Nagase T, Yoshihara H, Hayashi E, Imai Y, Kato M, Kashihara T, Hayashi N
  • Journal Title: J Gastroenterol Volume: 44(6) Pages: 601-7
  • Peer Reviewed
• [Journal Article] Visceral obesity and hypoadiponectinemia are significant determinants of hepatic dysfunction : An epidemiologic study of 3827 Japanese subjects (2009)
  • Author(s): Kamada Y, Nakamura T, Funahashi T, Ryo M, Nishizawa H, Okauchi Y, Fukushima J, Yoshida Y, Kiso S, Shimomura I, Hayashi N
  • Journal Title: J Clin Gastroenterol Volume: 43(10) Pages: 995-1000
  • Peer Reviewed
• [Journal Article] A novel therapeutic strategy with anti-CD9 antibody in gastric cancers (2009)
  • Author(s): Nakamoto T, Murayama Y, Oritani K, Boucheix C, Rubinstein E, Nishida M, Katsube F, Watabe K, Kiso S, Tsutsui S, Tamura S, Shinomura Y, Hayashi N
  • Journal Title: J Gastroenterol Volume: 44(9) Pages: 889-96
  • Peer Reviewed
• [Journal Article] Lamivudine-to-entecavir switching treatment in type B chronic hepatitis patients without evidence of lamivudine resistance (2009)
  • Author(s): Kurashige N, Ohkawa K, Hiramatsu N, Yakushijin T, Mochizuki K, Oze T, Kiso S, Kanto T, Takehara T, Kasahara A, Doi Y, Yamada A, Fukuda K, Oshita M, Mita E, Fukui H, Nagase T, Yoshihara H, Imai Y, Kato M, Kashihara T, Hayashi N
  • Journal Title: J Gastroenterol Volume: 44(8) Pages: 864-70
  • Peer Reviewed
• [Journal Article] Adiponectin prevents progression of steatohepatitis in mice by regulating oxidative stress and Kupffer cell phenotype polarization (2009)
  • Author(s): Fukushima J, Kamada Y, Matsumoto H, Yoshida Y, Ezaki H, Takemura T, Saji Y, Igura T, Tsutsui S, Kihara S, Funahashi T, Shimomura I, Tamura S, Kiso S, Hayashi N
  • Journal Title: Hepatol Res Volume: 39(7) Pages: 724-38
  • Peer Reviewed
• [Journal Article] Pegylated interferon alpha-2b(Peg-IFN alpha-2b) affects early virologic response dose-dependently in patients with chronic hepatitis C genotype 1 during treatment with Peg-IFN alpha-2b plus ribavirin (2009)
  • Author(s): Oze T, Hiramatsu N, Yakushijin T, Kurokawa M, Igura T, Mochizuki K, Imanaka K, Yamada A, Oshita M, Hagiwara H, Mita E, Ito T, Inui Y, Hijioka T, Tamura S, Yoshihara H, Hayashi E, Inoue A, Imai Y, Kato M, Yoshida Y, Tatsumi T, Ohkawa K, Kiso S, Kanto T, Kasahara A, Takehara T, Hayashi N
  • Journal Title: J Viral Hepat Volume: 16(8) Pages: 578-85
  • Peer Reviewed
• [Journal Article] Ribavirin dose reduction raises relapse rate dose-dependently in genotype 1 patients with hepatitis C responding to pegylated interferon alpha-2b plus ribavirin (2009)
  • Author(s): Hiramatsu N, Oze T, Yakushijin T, Inoue Y, Igura T, Mochizuki K, Imanaka K, Kaneko A, Oshita M, Hagiwara H, Mita E, Nagase T, Ito T, Inui Y, Hijioka T, Katayama K, Tamura S, Yoshihara H, Imai Y, Kato M, Yoshida Y, Tatsumi T, Ohkawa K, Kiso S, Kanto T, Kasahara A, Takehara T, Hayashi N
  • Journal Title: J Viral Hepat Volume: 16(8) Pages: 586-94
  • Peer Reviewed
• [Presentation] Takehara T Protective role of heparin-binding EGF-like growth factor during liver fibrosis in mice (2011)
  • Author(s): Takemura T, Yoshida Y, Kizu T, Furuta K, Kamada Y, Hamano M, Tsubakio M, Chatani N, Ezakki H, Kiso S
  • Organizer: 2011年米国肝臓病学会(AASLD)
  • Place of Presentation: サンフランシスコ、米国
  • Year and Date: 20111104-08
• [Presentation] Takehara T GAB1 scaffolding adaptor protein protects from liver fibrosis during chronic liver injury in mice (2011)
  • Author(s): Kizu T, Yoshida Y, Takemura T, Furuta K, Kamada Y, Chatani N, Hamano M, Tsubakio M, Ezaki H, Kiso S
  • Organizer: 2011年米国肝臓病学会(AASLD)
  • Place of Presentation: サンフランシスコ、米国
  • Year and Date: 20111104-08
• [Presentation] 肝再生・肝線維化進展過程におけるGab1の意義と肝幹/前駆細胞の関与 (2011)
  • Author(s): 吉田雄一、木曽真一、木津崇、竹原徹郎
  • Organizer: 第15回日本肝臓学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-10-21
• [Presentation] 肝細胞癌におけるGab1の関与と肝細胞癌治療の新規分子標的としての可能性 (2011)
  • Author(s): 吉田雄一、木曽真一、椿尾真弓、木津崇、茶谷徳啓、古田訓丸、濱野美奈、竹村貴代、江崎久男、鎌田佳宏、竹原徹郎
  • Organizer: 第15回日本肝臓学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-10-21
• [Presentation] Norio HayashiGaB1 scaffolding adaptor protein is required for stem/ progenitor cell mediated liver Regeneration in mice (2010)
  • Author(s): Yuichi Yoshida, Takashi Kizu, Mayumi Tsubakio, Norihiro Chatani, Mina HamanoTakayo Takemura, Hisao Ezaki, Yoshihiro Kamada, Shinichi Kiso
  • Organizer: AASLD(ボストン)
  • Place of Presentation: ボストン、米国
  • Year and Date: 20101029-1102
• [Presentation] Suppressive effect of pegylated interferon alfa-2B plus ribavirin combination therapy on incidence of hepatocellular carcinoma in patients with chronic hepatitis C (2010)
  • Author(s): Tugiko Oze, Naoki Hiramatsu, Takayuki Yakushijin, Kiyoshi Mochizuki, Masahide Oshita, Hideki Hagiwara, Eiji Mita, Hiroyuki Fukui, Yoshiaki Inui, Taizo Hijioka, Masami Inada, Shinji Tamura, Harumasa Yoshihara, Atsuo Inoue, Yasuharu Imai, Michio Kato, Atsushi Hosui, Takuya Miyagi, Yuichi Yoshida, Tomohide Tatsumi, Shinichi Kiso, Tatsuya Kanto, Akinori Kasahara, Tetsuo Takehara, Norio Hayashi
  • Organizer: AASLD(ボストン)
  • Place of Presentation: ボストン、米国
  • Year and Date: 20101029-1102
• [Presentation] HB-EGF conditional loss of liver accelerates the carbon tetrachloride induced liver injury in mice (2010)
  • Author(s): Takayo Takemura, Yuichi Yoshida, Yukiko Saji, Hisao Ezaki, Mina Hamano, Takashi Kizu, Mayumi Tsubakio, Norihiro Chatani, Yoshihiro Kamada, Shinichi Kiso, Norio Hayashi
  • Organizer: AASLD(ボストン)
  • Place of Presentation: ボストン、米国
  • Year and Date: 20101029-1102
• [Presentation] Norio Hayashi Estrogen deficiency enhanced the progression of steatohepatitis with increased macrophage infiltration in mice fed with high fat and high cholesterol diet (2010)
  • Author(s): Yoshihiro Kamada, Shinichi Kiso, Yuichi Yoshida, Hisao Ezaki, Takayo Takemura, Mina Hamano, Norihiro Chatani, Takashi Kizu, Mayumi Tsubakio
  • Organizer: AASLD(ボストン)
  • Place of Presentation: ボストン、米国
  • Year and Date: 20101029-1102
• [Presentation] Norio Hayashi Non-invasive evaluation of liver fibrosis by using virtual touch tissue quantification in patients with chronic hepatitis C (2010)
  • Author(s): Changho Song, Naoki Hiramatsu, Yuko Inoue, Mika Kurokawa, Tsugiko Oze, Takayuki Yakushijin, Kiyoshi Mochizuki, Takuya Miyagi, Yuichi Yoshida, Tomohide Tatsumi, Shinichi Kiso, Tatsuya Kanto, Tetsuo Takehara
  • Organizer: AASLD(ボストン)
  • Place of Presentation: ボストン、米国
  • Year and Date: 20101029-1102
• [Presentation] アダプター蛋白Gab1は、肝再生過程における肝幹/前駆細胞の関与 (2010)
  • Author(s): 吉田雄一、木曽真一、林紀夫
  • Organizer: 第14回日本肝臓学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20101013-14
• [Presentation] エストロゲン欠乏状態(閉経後)における高コレステロール血症のNASH病態進展への関与 (2010)
  • Author(s): 鎌田佳宏、木曽真一、林紀夫
  • Organizer: 第14回日本肝臓学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20101013-14
• [Presentation] Peg-IFN/ Ribavirin非著効例に対する再治療について-多施設(OLF)共同研究- (2010)
  • Author(s): 小瀬嗣子、平松直樹、薬師神崇行、望月圭、萩原秀紀、三田英治、田村信司、吉原治正、今井康陽、林英二朗、吉田雄一、巽智秀、木曽真一、考藤達哉、笠原彰紀、竹原徹郎、林紀夫
  • Organizer: 第14回日本肝臓学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20101013-14
• [Presentation] Peg-IFN/ Ribavirin併用療法の肝発癌制御効果について-多施設(OLF)共同研究- (2010)
  • Author(s): 小瀬嗣子、平松直樹、薬師神崇行、望月圭、尾下正秀、三田英治、吉原治正、今井康陽、木曽真一、考藤達哉、笠原彰紀、竹原徹郎、林紀夫
  • Organizer: 第14回日本肝臓学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20101013-14