2011 Fiscal Year Final Research Report
Identification of molecular chaperones as regulators for HCV RNA replication through proteomics approaches
| Project/Area Number |
21590867
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
AIZAKI Hideki 国立感染症研究所, ウイルス第二部, 室長 (00333360)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Teturou 浜松医科大学, 感染症講座, 教授 (00250184)
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| Project Period (FY) |
2009 – 2011
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| Keywords | ウイルス / 感染症 / 癌 / 内科 / プロテオーム |
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| Research Abstract |
To identify the host factors implicated in the regulation of hepatitis C virus(HCV) genome replication, we performed comparative proteome analyses of HCV replication complex(RC)-rich membrane fractions prepared from cells harboring genome-length bicistronic HCV RNA at the exponential and stationary growth phases. We found that CCT plays a role in the replication possibly through an interaction between subunit CCT5 and the viral RNA polymerase NS5B. siRNA-mediated knockdown of CCT5suppressed RNA replication. These suggest that recruitment of the chaperonin by the viral nonstructural proteins to the RC, which potentially facilitate folding of the RC component(s) into the mature active form, may be important for ef! cient replication of the HCV genome.
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[Journal Article] Chaperonin TRiC/CCT participates in replication of hepatitis C virus genome via interaction with the viral NS5B protein2011
Author(s)
Inoue Y, Aizaki H, Hara H, Matsuda M, Ando T, Shimoji T, Murakami K, Masaki T, Shoji I, Homma S, Matsuura Y, Miyamura T, Wakita T, Suzuki T
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Journal Title
Virology
Volume: 410
Pages: 38-47
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[Journal Article] Production of infectious hepatitis C virus by using RNA polymerase I-mediated transcription2010
Author(s)
Masaki T, Suzuki R, Saeed M, Mori KI, Matsuda M, Aizaki H, Ishii K, Maki N, Miyamura T, Matsuura Y, Wakita T, Suzuki T
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Journal Title
J Virol
Volume: 84
Pages: 5824-5835
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[Journal Article] Proteomics analysis of mitochondrial proteins reveals overexpression of a mitochondrial protein chaperone, prohibitin, in cells expressing hepatitis C virus core protein2009
Author(s)
Tsutsumi T, Matsuda M, Aizaki H, Moriya K, Miyoshi H, Fujie H, Shintani Y, Yotsuyanagi H, Miyamura T, Suzuki T, Koike K
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Journal Title
Hepatology
Volume: 50
Pages: 378-386
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[Presentation] Identification of lipid droplet-associated membrane proteins that are involved in HCV production2011
Author(s)
Aizaki H, Matsumoto Y, Goto K, Watashi K, Suzuki R, Fukasawa M, Hanada K, Sato S, Takahashi N, Matsuura Y, Motojima K, Miyamura T, Suzuki T, WakitaT
Organizer
18^<th> International Meeting on Hepatitis C Virus and Related Viruses
Place of Presentation
WA, USA
Year and Date
20110000
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[Presentation] Identification of novel NS5A-associated proteins in the host-cell membrane fraction and their role in HCV life cycle2011
Author(s)
Goto K, Kimura T, Watashi K, Suzuki R, Yamagoe S, Miyamura T, Moriya K, Yotsuyanagi H, Koike K, Suzuki T, Wakita T, Aizaki H
Organizer
18^<th> International Meeting on Hepatitis C Virus and Related Viruses
Place of Presentation
WA, USA
Year and Date
20110000
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[Presentation] Identification of lipid droplet-associated membrane proteins that are involved in HCV production2009
Author(s)
Aizaki H, Yamamoto M, Goto K, Fukasawa M, Hanada K, Sato S, Takahashi N, Matsuura Y, Miyamura T, Wakita T, Suzuki T
Organizer
16^<th> International Meeting on Hepatitis C Virus and Related Viruses
Place of Presentation
Nice, France
Year and Date
20090000
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