Mechanisms for Ca2+ absorption by pancreatic ductal epithelium : A study looking for a new strategy to prevent pancreatic stone formation.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590873
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Nagoya University
• Principal Investigator: ISHIGURO Hiroshi 名古屋大学, 総合保健体育科学センター, 教授 (90303651)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Akiko 名古屋大学, 総合保健体育科学センター, 准教授 (60402385) ; KO Shigeru 国立長寿医療研究センター, 病院・内科総合診療部 (90402578) ; NAGAO Shizuko 藤田保健衛生大学, 疾患モデル教育研究センター, 准教授 (20183527)
• Project Period (FY): 2009 – 2011
• Keywords: 膵石症 / 膵導管上皮 / カルシウム輸送体 / 単離小葉間膵管 / microperfusion / 多発性嚢胞腎症 / PCKラット / 線毛

Research Abstract

Pancreatic juice is an alkaline fluid that contains digestive enzymes and high concentrations of HCO3-. A little amount of CO32-in the juice binds to acinar cell-derived Ca2+, which leads to the precipitation of CaCO3. Pancreatic ductal epithelium is supposed to absorb luminal Ca2+ to prevent the formation of pancreatic stone. In the present study, we identified the membrane localization of Ca2+ transporters, measured the Ca2+-transport activities, and demonstrated the mechanisms to absorb luminal Ca2+ by pancreatic ductal epithelium.

Research Products

• [Journal Article] Physiology and path physiology of bicarbonate secretion by pancreatic duct epithelium (2012)
  • Author(s): Ishiguro H, Yamamoto A, Nakakuki M, Yi L, Ishiguro M, Yamaguchi M, Kondo S, Mochimaru Y.
  • Journal Title: Nagoya J Med Sci Volume: 74 Pages: 1-18
  • URL: http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7412/01_Ishiguro.pdf
  • Peer Reviewed
• [Journal Article] Pancreatic stone protein/regenerating protein family in pancreatic and gastrointestinal diseases. (2011)
  • Author(s): Jin CX, Hayakawa T, Ko SB, Ishiguro H, Kitagawa M.
  • Journal Title: Intern Med Volume: 50 Pages: 1507-16
  • DOI: DOI:10.2169/internalmedicine.50.5362
  • Peer Reviewed
• [Journal Article] SLC26 anion exchangers of guinea pig pancreatic duct : molecular cloning and functional characterization. (2011)
  • Author(s): Stewart AK, Shmukler BE, Vandorpe DH, Reimold F, Heneghan JF, Nakakuki M, Akhavein A, Ko SB, Ishiguro H, Alper SL
  • Journal Title: Stewart AKCell Physiol Volume: 301 Pages: 289-303
  • DOI: DOI:10.1152/ajpcell.00089.2011
  • Peer Reviewed
• [Journal Article] Effects of CFTR gene silencing by siRNA or the luminal application of a CFTR activator on fluid secretion from guinea-pig pancreatic duct cells (2011)
  • Author(s): Ko SB, Yamamoto A, Azuma S, Song H, Kamimura K, Nakakuki M, Gray MA, Becq F, Ishiguro H, Goto H.
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Pages: 904-9
  • URL: http://dx.doi.org/10.1016/j.bbrc.2011.06.093
  • Peer Reviewed
• [Journal Article] Microperfusion and micropuncture analysis of ductal secretion (2011)
  • Author(s): Ishiguro H, Steward MC, Yamamoto A.
  • Journal Title: University of Michigan Library
  • DOI: DOI:10.3998/panc.2011.16
  • Peer Reviewed
• [Journal Article] Corticosteroids correct aberrant CFTR localization in the duct and regenerate acinar cells in autoimmune pancreatitis (2010)
  • Author(s): Ko SB, Mizuno N, Yatabe Y, Yoshikawa T, Ishiguro H, Yamamoto A, Azuma S, Naruse S, Yamao K, Muallem S, Goto H.
  • Journal Title: Gastroenterology Volume: 138 Pages: 1988-96
  • DOI: DOI:10.1053/j.gastro.2010.01.001
  • Peer Reviewed
• [Journal Article] High glucose inhibits HCO3-and fluid secretion in rat pancreatic ducts (2009)
  • Author(s): Futakuchi S, Ishiguro H, Naruse S, Ko SB, Fujiki K, Yamamoto A, Nakakuki M, Song Y, Steward MC, Kondo T, Goto H.
  • Journal Title: Pflugers Arch Volume: 459 Pages: 215-26
  • DOI: DOI:10.1007/s00424-009-0731-6
  • Peer Reviewed
• [Journal Article] CFTR functions as a bicarbonate channel in pancreatic duct cells (2009)
  • Author(s): Ishiguro H, Steward MC, Naruse S, Ko SB, Goto H, Case RM, Kondo T, Yamamoto A.
  • Journal Title: J Gen Physiol Volume: 133 Pages: 315-26
  • DOI: DOI:10.1085/jgp.200810122
  • Peer Reviewed
• [Journal Article] Functional coupling of apical Cl-/HCO-(3)-exchange with CFTR in stimulated HCO-(3)-secretion by guinea pig interlobular pancreatic duct (2009)
  • Author(s): Yamamoto A, Nakakuki M, Kondo T, Alper SL, Ishiguro H.
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 269 Pages: 1307-17
  • DOI: DOI:10.1152/ajpgi.90697.2008
  • Peer Reviewed
• [Journal Article] Molecular and cellular regulation of pancreatic duct cell function (2009)
  • Author(s): Steward MC, Ishiguro H.
  • Journal Title: Curr Opin Gastroenterol Volume: 25 Pages: 447-53
  • DOI: DOI:10.1097/MOG.0b013e32832e06ce
  • Peer Reviewed
• [Journal Article] Structure and function of the pancreas in the polycystic kidney rat
  • Author(s): Yi L, Naruse S, Furuya S, Yamamoto A, Nakakuki M, Nagao S, Yoshihara D, Ko SB, Wei M, Kondo T, Ishiguro H.
  • Journal Title: Pancreas
  • Peer Reviewed
• [Presentation] Pancreatic duct dysfunction and (2011)
  • Author(s): Ishiguro H
  • Organizer: Gordon Research Conferences
  • Place of Presentation: Salivary Glands & Exocrine Biology(Galveston)
  • Year and Date: 20110000
• [Presentation] Molecular and cellular regulation of pancreatic duct function (2010)
  • Author(s): Ishiguro H, Ko SB, Yamamoto A.
  • Organizer: Joint Meeting of the International Association of Pancreatology and the Japan Pancreas Society
  • Place of Presentation: Fukuoka
  • Year and Date: 20100000
• [Presentation] 膵導管細胞において膵石形成に関わるカルシウム輸送体の検討 (2010)
  • Author(s): 石黒真理子、石黒洋、山本明子、藤木理代、北川元二、近藤孝晴
  • Place of Presentation: 第64回日本栄養・食糧学会大会(徳島)
  • Year and Date: 20100000
• [Presentation] Fluid secretion from the pancreatic duct in PCK rats (2010)
  • Author(s): Yi L, Ishiguro H, Yamamoto A, Nakauki M, Furuya S, Nagao S
  • Organizer: Joint Meeting of the International Association of Pancreatology and the Japan Pancreas Society
  • Place of Presentation: Fukuoka
  • Year and Date: 20100000
• [Presentation] 膵導管細胞におけるカルシウム輸送体の発現と局在の検討 (2009)
  • Author(s): 石黒真理子、石黒洋、山本明子、藤木理代、北川元二、近藤孝晴
  • Organizer: 第40回日本膵臓学会大会
  • Place of Presentation: 東京
  • Year and Date: 20090000