2011 Fiscal Year Final Research Report
Role of serum mass and activity of endothelial lipase in serum high-density lipoprotein cholesterol level in humans
| Project/Area Number |
21590897
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kobe University |
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Principal Investigator |
ISHIDA Tatsuro 神戸大学, 医学部附属病院, 准教授 (00379413)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATA Ken-ichi 神戸大学, 大学院・医学研究科, 教授 (20283880)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 脂質代謝 / 循環器 / 血管内皮 / リポ蛋白代謝 / リパーゼ / 脂質異常症 |
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| Research Abstract |
High-density lipoprotein cholesterol(HDL-C) has a variety of athero-protective actions. Endothelial lipase(EL) hydrolyzes phospholipids in the HDL particle and promotes its catabolism. However, the role of EL in HDL-C levels and atherosclerosis in humans has not been fully elucidated. In this study, we further investigated the role of plasma concentration and activity of EL in HDL-C levels in human subjects.
We have established a novel ELISA system for plasma EL mass by using two monoclonal antibodies against human EL. EL-specific plasma phospholipase activity was measured using a fluorogenic phospholipid as a substrate, after plasma EL protein was immunopricipited using anti-EL antibodies. The plasma EL mass and activity were inversely correlated with plasma HDL-C levels. In addition, the EL mass or activity in patients with cardiovascular diseases was significantly higher than those in patients with cardiovascular diseases. The EL mass or activity was correlated with coronary risk factors including cigarette smoking, high-sensitive CRP, and dyslipidemia.
In conclusion, plasma EL mass and activity are determinants of plasma HDL-C levels, and correlated with risks in cardiovascular diseases. Because inhibition of plasma EL activity increased anti-inflammatory HDL particles, EL may represent a novel target for HDL-raising pharmaceutical interventions for patients with low HDL levels.
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[Journal Article] Endothelial lipase mediates hdl levels in normal and hyperlipidemic rabbits2012
Author(s)
Zhang J, Yu Y, Nakamura K, Koike T, Waqar AB, Zhang X, Liu E, Nishijima K, Kitajima S, Shiomi M, Qi Z, Yu J, Graham MJ, Crooke RM, Ishida T, Hirata K, Hurt-Camejo E, Chen YE, Fan J
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Journal Title
J Atheroscler Thromb
Volume: 19(3)
Pages: 213-26
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[Journal Article] Orally Administered Eicosapentaenoic Acid Induces Rapid Regression of Atherosclerosis Via Modulating the Phenotype of Dendritic Cells in LDL Receptor-Deficient Mice2011
Author(s)
Nakajima K, Yamashita T, Kita T, Takeda M, Sasaki N, Kasahara K, Shinohara M, Rikitake Y, Ishida T, Yokoyama M, Hirata K
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Journal Title
Arterioscler Thromb Vasc Biol
Volume: 31(9)
Pages: 1963-72
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[Journal Article] Pitavastatin reduces elevated IL-18 levels in Japanese subjects with hypercholesterolemia : Sub-analysis of Kansai Investigation of Statin for Hyperlipidemic Intervention in Metabolism and Endocrinology(KISHIMEN)2011
Author(s)
Fujioka Y, Fukuda A, Ishida T, Kagimoto S, Nakamura Y, Iwakura A, Hara K, Yamamoto T, Kuroe A, Ohya M, Fujimoto S, Hamamoto Y, Honjo S, Ikeda H, Nabe K, Tsuda K, Taniguchi A, Tanaka K, Koshiyama H, Kume N, Hirata K
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Journal Title
J Atheroscler Thromb
Volume: 18(1)
Pages: 8-15
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[Journal Article] Endothelial cell-selective adhesion molecule modulates atherosclerosis through plaque angiogenesis and monocyteendothelial interaction2010
Author(s)
Inoue M, Ishida T, Yasuda T, Toh R, Hara T, Cangara HM, Rikitake Y, Taira K, Sun L, Kundu RK, Quertermous T, Hirata K
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Journal Title
Microvasc Res
Volume: 80(2)
Pages: 179-87
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[Journal Article] Pitavastatin decreases expression of endothelial lipase both in vitro and in vivo2010
Author(s)
Kojima Y, Ishida T, Sun L, Yasuda T, Toh T, Rikitake Y, Fukuda A, Kume N, Koshiyama H, Taniguchi A, Hirata K
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Journal Title
Cardiovasc Res
Volume: 87(2)
Pages: 385-93
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[Presentation] Establishment of a novel, high-sensitive ELISA system for human endothelial lipase2012
Author(s)
Ishida, T, Miyashita, K, Kinoshita, N, Seito, S, Hirata K, Nakajima K.
Organizer
HDL Satellite symposium 2012
Place of Presentation
Cairns, Australia
Year and Date
20120330-0401
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[Presentation] Cardiac endothelial lipase expression is augmented in the early stage of heart failure2012
Author(s)
Nakajima H, Ishida T, Sasaki M, Kondo K, Mori K, Hasokawa M, Haraguchi Y, Yasuda T, Kobayashi S, Hirata K
Organizer
The XVI International Symposium on Atherosclerosis(ISA2012)
Place of Presentation
Sydney, Australia. Poster
Year and Date
2012-03-27
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[Presentation] Cardiac endothelial lipase expression is augmented in a murine model of heart failure2012
Author(s)
Nakajima H, Ishida T, Monguchi T, Sasaki M, Kondo K, Mori K, Hasokawa M, Haraguchi Y, Sun L, Yasuda T, Shinohara M, Toh R, Kobayashi S, Hirata K
Organizer
第76回日本循環器学会学術集会
Place of Presentation
福岡
Year and Date
2012-03-17
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