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2012 Fiscal Year Final Research Report

The development and clinical application of a new oxidative stress marker using glutathionylated protein

Research Project

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Project/Area Number 21590902
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionNagasaki University

Principal Investigator

IKEDA Satoshi  長崎大学, 医歯薬学総合研究科, 講師 (10336159)

Co-Investigator(Kenkyū-buntansha) MAEMURA Koji  長崎大学, 医歯薬学総合研究科, 教授 (90282649)
URATA Yoshishige  長崎大学, 医歯薬学総合研究科, 助教 (30185087)
Project Period (FY) 2009 – 2012
Keywords臨床心血管病態学
Research Abstract

Oxidative stress contributes to the development and progression of atherosclerosis. Reactive oxygen species, major sources of oxidative stress, modifies protein cysteine residues, where a disulfide bond is established between a cysteinyl residue and glutathione, leading to the S-glutathionylation of protein, and this modification can be still reversible. Therefore, we hypothesized a biomarker using S-glutathionylated protein is a good one reflecting an early phase of atherosclerosis. We composed the antibody against S-glutathionylation of apoprotein B100, a component of low-density lipoprotein (LDL) cholesterol. The serum values by immunoblot analysis with this antibody were correlated with negatively charged LDL subfractions, which are induced by oxidative modification, in patients with atherogenetic risks, such as hypertension, diabetes mellitus, and/or dyslipidemia. Immunohistological analysis of carotid endarterectomy sections with the antibody showed S-glutationylated ApoB100 localized at the site close to the vascular lumen, but not the site of foamy cell accumulation. These suggested that serum S-glutationylated ApoB100 may be an oxidative stress marker reflecting the relatively early phase of atherosclerosis.

  • Research Products

    (6 results)

All 2012 2011 2010

All Journal Article (1 results) Presentation (5 results)

  • [Journal Article] Negatively charged low-density lipoprotein with atherogenic risk in hypertensive patients2012

    • Author(s)
      Urata J, Ikeda S, Koga S, Nakata T, Yasunaga T, Sonoda K, Koide Y, Ashizawa N, Kohno S, Maemura K
    • Journal Title

      Heart and Vessels

      Volume: 27 Pages: 235-242

    • DOI

      DOI10.1007/s00380-011-0139-z

  • [Presentation] S-glutathionylated Apoprotein B100 is related with electronegativity of LDL in patients with atherogenic risks2011

    • Author(s)
      Ikeda S, Koga S, Urata Y, Urata J, NakataT,Yasunaga T, Maemura K
    • Organizer
      The 75th Annual Scientific Meeting of the Japanese Circulation Society
    • Place of Presentation
      Yokohama
    • Year and Date
      2011-08-03
  • [Presentation] Is S-glutathionylated Apoprotein B100 a potential marker for atherogenic risk?2010

    • Author(s)
      Ikeda S, Koga S, Urata Y, Urata J, Nakata T, Yasunaga T, Maemura K
    • Organizer
      AHA Scientific Sessions 2010
    • Place of Presentation
      Chicago, US
    • Year and Date
      2010-11-14
  • [Presentation] Anion-exchange high performance liquid chromatographyを用いた陰性荷電LDLの臨床的意義の検討2010

    • Author(s)
      池田聡司、古賀聖士、浦田淳吾、安永智彦、中田智夫、前村浩二
    • Organizer
      第33回日本高血圧学会総会
    • Place of Presentation
      福岡
    • Year and Date
      2010-10-15
  • [Presentation] 高血圧患者におけるAnion-exchange HPLCを用いた陰性荷電LDL分画の臨床的意義の検討2010

    • Author(s)
      池田聡司、古賀聖士、浦田淳吾、安永智彦、中田智夫、前村浩二
    • Organizer
      第58回日本心臓病学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-17
  • [Presentation] Low-density lipoprotein subfractions isolated by anionexchange high-performance chromategraphy are associated with recent atherogenic risk factors in hypertensive patients2010

    • Author(s)
      Ikeda S, Koga S, Urata J, Yasunaga T, Nakata T, Maemura K
    • Organizer
      第42回日本動脈硬化学会・学術集会
    • Place of Presentation
      岐阜
    • Year and Date
      2010-07-16

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Published: 2014-08-29  

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