2010 Fiscal Year Final Research Report
Challenges to cell specific epigenetics in the lungs
| Project/Area Number |
21590961
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Hokkaido University |
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Principal Investigator |
BETSUYAKU Tomoko Hokkaido University, 大学院・医学研究科, 准教授 (60333605)
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| Project Period (FY) |
2009 – 2010
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| Keywords | LCM / マウス / 喫煙 / 細気管支上皮 / miRNA |
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| Research Abstract |
MicroRNAs (miRNAs) are a family of non-coding RNA molecules that post-transcriptionally regulate gene expression. It has recently been discovered that changes in expression of miRNAs are involved in development, carcinogenesis and inflammation. However, it is largely unknown how aging and smoking are associated with differential expression of miRNAs in the lungs. The main problem with analyzing miRNA expression in the lungs is the degree of cellular heterogeneity. The use of tissue homogenates inevitably results in average expression levels of all cells present in the lungs, thus potentially masking any differences in the expression of individual miRNAs within specific cell types. We therefore focused on the changes in miRNA expression in bronchiolar epithelium in vivo, since bronchiolar epithelium is an important site in the pathophysiology of COPD as well as in cigarette smoke (CS)-induced animal models of the disease. We demonstrated changes in miRNAs in mouse bronchiolar epithelium by age and CS exposure. We also found that the cessation of smoking differently affected the miRNA profiling by age.
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[Journal Article] Cigarette smoke induces nucleic acid oxidation in lung fibroblasts2010
Author(s)
Deslee G, Adair-Kirk TL, Betsuyaku T, Woods JC, Moore CH, Gierada DS, Conradi SH, Atkinson JJ, Toennies HM, Battaile JT, Kobayashi DK, Patterson GA, Holtzman MJ, Pierce RA
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Journal Title
Am J Respir Cell Mol Biol 43(5)
Pages: 576-584
Peer Reviewed
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[Journal Article] Laminin gamma2 fragments are increased in the circulation of patients with early phase acute lung injury2010
Author(s)
Katayama M, Ishizaka A, Sakamoto M, Fujishima S, Sekiguchi K, Asano K, Betsuyaku T, Kotani T, Ware LB, Matthay MA, Hashimoto S
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Journal Title
Intensice Care Med 36(3)
Pages: 479-486
Peer Reviewed
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[Presentation] Duration of cigarette smoke (CS) exposure affects the resolution of lung inflammation after cessation in mice2009
Author(s)
Odajima N, Betsuyaku T, Inomata T, Yoshida T, Moriyama C, Suzuki K, Nagai K, Nasuhara Y, Nishimura M
Organizer
The 105^<th> American Thoracic Society International Conference
Place of Presentation
The San Diego Convention Center, San Diego, CA, United States
Year and Date
2009-05-17
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