2011 Fiscal Year Final Research Report
Adipose tissue-derived stem/stromal cells(ASC) therapy to elastase-elicited pulmonary emphysema in rat model
| Project/Area Number |
21590976
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Respiratory organ internal medicine
|
|---|
| Research Institution | St. Marianna University School of Medicine |
|---|
Principal Investigator |
TAKENAGA Mitsuko 聖マリアンナ医科大学, 医学(系)研究科(研究院), 准教授 (10236490)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OHTA Yuki 聖マリアンナ医科大学, 薬理学, 助教 (60387066)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | 閉塞性肺疾患 |
|---|
| Research Abstract |
Adipose tissue-derived stem/stromal cells(ASC) transplantation(2.5×10^6/500μl/20min) was performed through intravenous route in porcine pancreatic elastase(PPE)(250 U/kg)-induced emphysema model rats. Pulmonary function(arterial oxygen tension(PaO_2) and alveolar-arterial oxygen tension difference(A-aDO_2) was restored almost to that of normal animals. Enlargement of the alveolar airspaces was inhibited. Hepatocyte growth factor(HGF) and cytokine-induced neutrophil chemoattractant-1(CINC-1) levels were significantly higher in the ASC group even at 2 weeks after transplantation. Sponge implantation with CINC-1 induced neovascular formation with increased HGF. In vitro secretion of HGF and CINC-1 from ASC was promoted in the presence of IL-1β. Some grafted ASC was confirmed to survive at the damaged site. Therefore, not only HGF but also CINC-1 secreted from transplanted and viable ASC, presumably contributed to lung repair through angiogenesis.
|
Research Products
(11 results)
-
[Journal Article] Pulmonary functions, angiogenic growth factors and related ones, and histological changes in elastase-induced pulmonary emphysema rat model2011
Author(s)
Furuya N, Takenaga M, Ohta Y, Tokura Y, Hamaguchi A, Sakamaki A, Kida H, Handa H, Nishine H, Mineshita M, Miyazawa T
-
Journal Title
J. St. Marianna Univ
Volume: 2(2)
Pages: 71-77
Peer Reviewed
-
-
[Journal Article] Cell therapy with adipose tissue-derived stem/stromal cells(ASC) for elastase-induced pulmonary emphysema in rats
Author(s)
Furuya N, Takenaga M, Ohta Y, Tokura Y, Hamaguchi A, Sakamaki A, Kida H, Handa H, Nishine H, Mineshita M, Miyazawa M
-
Journal Title
Regenerative Med
Volume: (in press)
Peer Reviewed
-
-
-
-
-
-
-
-
