Clarification of causal antigens of bronchial asthma in terms of cellular immune responses and mediators of bronchoconstriction

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590978
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital
• Principal Investigator: MORI Akio 独立行政法人国立病院機構(相模原病院臨床研究センター), 臨床研究センター, 部長 (80251247)
• Co-Investigator(Kenkyū-buntansha): OHTOMO Takayuki 独立行政法人国立病院機構相模原病院, 臨床研究センター, 特別研究員 (90463108)
• Project Period (FY): 2009 – 2011
• Keywords: 閉塞性肺疾患 / 気管支喘息 / アレルゲン / T細胞 / 遅発型喘息反応 / 細胞性免疫 / IL-5

Research Abstract

Mechanism of bronchoconstriction in nonatopic asthma is studied in the present study. T cell IL-5 production was induced in response to house dust mite or mold allergens despite lack of IgE antibodies. Upon bronchial provocation, late asthmatic response, but not immediate asthmatic response, was induced in IL-5-producing asthmatics. To delineate mechanisms of IgE-independent bronchoconstriction, T cell clones were established, and the bronchoconstriction activity of activated T cell clones was analyzed in terms of human bronchial smooth muscle cells-embedded collagen gels.

Research Products

• [Journal Article] Murine T cell-derived contractile activity for human bronchial smooth muscle cells (2012)
  • Author(s): Abe, A., Koyama, S., Ohtomo, T., Kitamura, N., Kaminuma, O., and Mori, A.
  • Journal Title: Int. Arch. Allergy Immunol Volume: 158 Pages: 7-10
  • DOI: DOI:10.1159/000337743
• [Journal Article] 重症喘息の病態と真菌抗原による非IgE依存性喘息反応、臨床免疫 (2011)
  • Author(s): 森晶夫
  • Journal Title: アレルギー科 Volume: 56(1) Pages: 44-50
  • URL: http://www.kahyo.com/category/A1-MA
• [Journal Article] Eosinophils are required for the induction of bronchial hyperresponsiveness in a Th transfer model of Balb/c background (2010)
  • Author(s): Ohtomo, T., Kaminuma, O., Yamada, J., Kitamura, N., Abe, A., Kobayashi, N., Suko, M., and Mori, A.
  • Journal Title: Int. Arch. Allergy Immunol Volume: 152 Pages: 79-82
  • DOI: DOI:10.1159/000312130
• [Presentation] Role of T cells in late phase asthmatic response (2010)
  • Author(s): Mori A, Kitamura N, Otomo T, Abe, A., and Kaminuma O.
  • Organizer: The 8th Asia pacific Congress of Allergy, Asthma, and Clinical Immunology 2010
  • Place of Presentation: Singapore
  • Year and Date: 20101106-09
• [Presentation] T cell-dependent bronchoconstriction in vivo and in vitro (2010)
  • Author(s): Mori A, Kitamura N, Otomo T, Abe, A., and Kaminuma O.
  • Organizer: European Association of Allergy and Clinical Immunology 2010
  • Place of Presentation: London
  • Year and Date: 20100605-09
• [Book] アトピー型喘息と非アトピー型喘息の病態機序、第30回六甲カンファレンス (2011)
  • Author(s): 森晶夫
  • Publisher: ライフサイエンス出版
• [Book] 非アトピー型喘息、The 17th Symposium of Asthma in Tokyo (2010)
  • Author(s): 森晶夫
  • Publisher: ライフサイエンス出版