2011 Fiscal Year Final Research Report
Development of system to predict postoperative recurrence of lung cancer/order-made treatment using peripheral blood circukationg tumor cell
| Project/Area Number |
21590983
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKATA Koh 新潟大学, 医歯学総合病院, 教授 (80207802)
WATANABE Satoshi 新潟大学, 医歯学総合病院, 特任助教 (50529740)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 肺癌 / 術後再発 / 末梢血循環腫瘍細胞 |
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| Research Abstract |
Whether circulating tumor cells(CTC) could be a prediction factor of the postoperative recurrence of lung cancer was investigated. Also CTC was utilized to establish order-made adjuvant chemotherapy. EpCAM、anti-cytokeratin mAb with magnetic beads were used to isolate CTC. Sensitivity of the method was raised to detect 1/100000 of PC9 tumor cells among normal leucocyte. CTC was examined in blood samples of advanced stage of lung cancer as well as post-operative patients. CTC was detected in 3% in advanced stage while no positive case in post-operative patients. The present condition is under re-examination for improvement in its sensitivity. ERCC gene family, beta-Tubulin, and RRM1 using Allele specific PCR and Real time PCR are under examination.
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