Cancer antigen WT1-targeting peptide vaccine therapy for lung cancer

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590991
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Osaka University
• Principal Investigator: OKA Yoshihiro 大阪大学, 大学院・医学系研究科, 講師 (20273691)
• Co-Investigator(Kenkyū-buntansha): TAKEDA Yoshito 大阪大学, 大学院・医学系研究科, 助教 (40452388)
• Project Period (FY): 2009 – 2011
• Keywords: WT1 / 肺癌 / 癌免疫療法 / ペプチドワクチン

Research Abstract

We accumulated cases with lung cancer treated by WT1 peptide vaccine. We analyzed anti-WT1 antibody in nonsmall cell lung cancer patients, which showed the existence of immune response against WT1 protein in the lung cancer. We analyzed WT1-specific CTLs in bone marrow(BM) of cancer-bearing patients, which indicated the possibility that BM had a role as a secondary lymhpoid organ in cancer immunity. We analyzed WT1 helper peptide-specific response of CD4^+ T cells, which suggested an important role of Th response in cancer immunity. We demonstrated, using a mice model, that co-administration of WT1 peptide vaccine and IFN-βwas a promising novel therapy for cancer. Biased usage of TCR BV gene families of WT1-specific CD8^+ T cells in patients with solid tumors and healthy donors was shown.

Research Products

• [Journal Article] Biased usage of T cell receptorβ-chain variable region genes of WT1(Wilms' tumor gene)-specific CD8^+ T cells in patients with solid tumors and healthy donors (2012)
  • Author(s): Morimoto S., Oka Y., Tsuboi A., Tanaka Y., Fujiki F., Nakajima H., Hosen N., Nishida S., Nakata J., Nakae Y., Murao A., Myoui A., Enomoto T., Izumoto S., Sekimoto M., Kagawa N., Hashimoto N., Yoshimine T., Oji Y., Kumanogoh A., Sugiyama H
  • Journal Title: Cancer Science Volume: 103 Pages: 408-414
  • Peer Reviewed
• [Journal Article] Enhanced tumor immunity of WT1 peptide vaccination by interferon-βadministration (2012)
  • Author(s): Nakajima H., Oka Y., Tsuboi A., Tatsumi N., Yamamoto Y., Fujiki F., Li Z., Murao A., Morimoto S., Hosen N., Shirakata T., Nishida S., Kawase I., Isaka Y., Oji Y., Sugiyama H
  • Journal Title: Vaccine Volume: 30 Pages: 722-729
  • Peer Reviewed
• [Journal Article] A clear correlation between WT1-specific Th response and clinical response in WT1 CTL epitope vaccination (2010)
  • Author(s): Fujiki F., Oka Y., Kawakatsu M., Tsuboi A., Harada Y., Hosen N., Nishida S., Shirakata T., Nakajima H., Tatsumi N., Hashimoto N., Taguchi T., Ueda S., Nonomura N., Takeda Y., Ito T., Myoui A., Izumoto Izumoto S., Maruno M., Yoshimine T., Noguchi S., Okuyama A., Kawase I., Oji Y., Sugiyama H
  • Journal Title: Anticancer Research Volume: 30 Pages: 2247-2254
  • Peer Reviewed
• [Journal Article] High frequencies of less differentiated and more proliferative WT1-specific CD8+ T cells in bone marrow in tumor-bearing patients : an important role of bone marrow as a secondary lymhpoid organ (2010)
  • Author(s): Murao A., Oka Y., Tsuboi A., Elisseeva O. A., Harada Y., Fujiki F., Nakajima H., Nishida S., Hosen N., Shirakata T., Hashimoto N., Myoui A., Ueda T., Takeda Y., Osaki T., Enomoto T., Yoshikawa H., Kimura T., Oji Y., Kawase I., Sugiyama H
  • Journal Title: Cancer Science Volume: 101 Pages: 848-854
  • Peer Reviewed
• [Journal Article] WT1 peptide vaccine as a paradigm for" cancer antigen-derived peptide"-based immunotherapy for malignancies : Successful induction of ant-cancer effect by vaccination with a single kind of WT1 peptide (2009)
  • Author(s): Oka Y., Tsuboi A., Fujiki F., Li Z., Nakajima H., Hosen N., Shirakata T., Nishida S., Oji Y., Kawase I., Sugiyama H
  • Journal Title: Anti-Cancer Agents in Medicinal Chemistry Volume: 9 Pages: 787-797
  • Peer Reviewed
• [Journal Article] WT1 IgG antibody for early detection of non-small cell lung cancer and as its prognostic factor (2009)
  • Author(s): Oji Y., Kitamura Y., Kamino E., Kitano A., Sawabata N., Inoue M., Mori M., Nakatsuka S-I., Sakaguchi N., Miyazaki K., Nakamura M., Fukuda I., Nakamura J., Tatsumi N., Takakuwa T., Nishida S., Shirakata T., Hosen N., Tsuboi A., Nezu R., Maeda H., Oka Y., Kawase I., Aozasa K., Okumura M., Miyoshi S., Sugiyama H
  • Journal Title: International Journal of Cancer Volume: 125 Pages: 381-387
  • Peer Reviewed
• [Presentation] 腫瘍抗原WT1特異的ヒトTh17細胞クローンの樹立と機能解析/ Establishment and characterization of tumor antigen WT1-specific human Th17 clone (2011)
  • Author(s): Tachino S, Fujiki F., Oka Y., Tsuboi A., Nakajima H., Hosen N., Oji Y., Kumanogoh A., Sugiyama H
  • Organizer: 第40回日本免疫学会学術総会
  • Place of Presentation: 千葉
  • Year and Date: 2011-11-28
• [Presentation] WT1 peptide vaccination combined with IFN-βenhances tumor rejection in a mice model (2011)
  • Author(s): Nakajima H., Oka Y., Tsuboi A., Tatsumi N., Fujiki F., Morimoto S., Hosen N., Shirakata T., Nishida S., Oji Y., Sugiyama H
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-04
• [Presentation] WT1 peptide vaccine for the treatment of malignancies. Symposium 1(Cancer Immunotherapy : Current Status and Future Prospects) (2010)
  • Author(s): Oka Y, Sugiyama H
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-24
• [Presentation] WT1 peptide vaccine for the treatment of solid and hematological malignancies. Symposium 10(Cancer and Immunity) (2010)
  • Author(s): Oka Y, Sugiyama H
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-22
• [Presentation] 担癌患者の骨髄中には末梢血に比較して癌抗原WT1特異的CD8^+ T細胞がより高頻度かつ未熟な状態で存在する (2010)
  • Author(s): 岡芳弘、村尾綾子、坪井昭博、田中ゆきえ、藤木文博、中島博子、西田純幸、保仙直毅、尾路祐介、杉山治夫
  • Organizer: 第14回日本がん免疫学会総会
  • Place of Presentation: 熊本
  • Year and Date: 2010-07-22
• [Presentation] ペプチドワクチン-WT1ペプチド癌ワクチンをモデルとして-.シンポジウム(「臓器移植の将来の展望-HLAの医療への関与-」 (2010)
  • Author(s): 岡芳弘
  • Organizer: 第8回日本組織適合性学会近畿地方会
  • Place of Presentation: 大阪
  • Year and Date: 2010-02-06