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2009 Fiscal Year Annual Research Report

肺癌における癌幹細胞形質マーカーおよび新規抗癌剤効果規定遺伝子の同定

Research Project

Project/Area Number 21590997
Research InstitutionHiroshima University

Principal Investigator

檜山 桂子  Hiroshima University, 原爆放射線医科学研究所, 准教授 (60253069)

Keywords肺癌 / 幹細胞 / 癌幹細胞 / Rb / p16経路 / p53 / テロメラーゼ / コロニー形成能 / マーカー
Research Abstract

肺癌組織83検体、肺癌細胞株15検体の遺伝子解析および細胞培養を行い、Rb/p16経路の不活化(RB1欠失・発現抑制、CDKN2S欠失・メチル化・発現低下)、p53経路の不活化(欠失・変異・発現低下)を経ずにテロメラーゼを活性化させて不死化していると考えられる肺癌細胞が特に腺癌において、高テロメラーゼ活性を示す検体の役半数に存在すること(肺腺癌組織42例中14例高テロメラーゼ活性、うち7例が両経路の不活化なし、低活性28例は全例不活化、P=0.0001)、この性質を示す肺癌細胞株(9肺腺癌細胞株中2株)は、両経路が不活化されている細胞に比し増殖能が同等でも軟寒天におけるコロニー形成能が高いことも見出した。うち一株は、さまざまながんでがん幹細胞マーカーであることが報告されてきたPROM1(CD133)を高発現しており、これらの結果をInt J. Oncol.に報告した(2010年5月掲載予定)。元来テロメラーゼが活性化されておらず有限寿命である正常体細胞は、Rb/p16経路およびTP53経路の不活化なしにはテロメラーゼを活性化させて不死化することができないことが報告されており、我々が見出した両経路が不活化されずに不死化している肺癌細胞は、元来テロメラーゼ活性を有している幹細胞に由来する癌細胞である可能性が示された。すなわち、テロメラーゼ陽性不死化がん細胞において両経路の不活化が存在しないことは、幹細胞由来の癌細胞、癌幹細胞由来である可能性も示された。

  • Research Products

    (52 results)

All 2010 2009

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (38 results) Book (6 results)

  • [Journal Article] Carcinogenesis and cellular immortalization without persistent inactivation of p16/Rb pathway in lung cancer2010

    • Author(s)
      Arifin M.
    • Journal Title

      International Journal of Oncology 36(5)

      Pages: 1217-1227

    • Peer Reviewed
  • [Journal Article] A robust method for estimating gene expression states using Affymetrix microarray probe level data2010

    • Author(s)
      Ohtaki M.
    • Journal Title

      BMC Bioinformatics (印刷中)

    • Peer Reviewed
  • [Journal Article] Activation of the hypoxia-inducible factor-1 in overloaded temporomandibular joint, and induction of osteoclastogenesis2010

    • Author(s)
      Shirakura M.
    • Journal Title

      Biochem Biophys Res Commun 393(4)

      Pages: 800-805

    • Peer Reviewed
  • [Journal Article] EMP3 as a candidate tumor suppressor gene for solid tumors. Expit Opin2009

    • Author(s)
      Fumoto S.
    • Journal Title

      Expert Opinion on Therapeutic Targets 13(7)

      Pages: 811-822

    • Peer Reviewed
  • [Journal Article] Detection of Island Hypermethylation of Caspase-8 in Neuroblastoma Using an Oligonucleotide Array2009

    • Author(s)
      Kamimatsuse A.
    • Journal Title

      Pediatr Blood Cancer 52(7)

      Pages: 777-783

    • Peer Reviewed
  • [Journal Article] Evaluation of genes identified by microarray analysis in favorable neuroblastoma2009

    • Author(s)
      Kamei N.
    • Journal Title

      Pediatr Surg Int 25(11)

      Pages: 931-937

    • Peer Reviewed
  • [Journal Article] ゲノム解析の臨床と研究 これを支える体制と整備 ゲノム・遺伝子研究と倫理2009

    • Author(s)
      檜山桂子
    • Journal Title

      日本癌治療学会誌 44(1)

      Pages: 1255-1259

    • Peer Reviewed
  • [Journal Article] Telomere biology in neuroblastoma : telomere binding proteins and alternative strengthening of telomeres2009

    • Author(s)
      Onitake Y
    • Journal Title

      J Pediatr Surg 44(12)

      Pages: 2258-2266

    • Peer Reviewed
  • [Presentation] Analysis of HIF-1-transgenic mice2010

    • Author(s)
      谷本圭司
    • Organizer
      International Symposium on the Innovation for Health and Sustainable Life
    • Place of Presentation
      Hiroshima
    • Year and Date
      2010-03-09
  • [Presentation] Activation of the hypoxia-inducible factor-1 in chondrocytes2010

    • Author(s)
      谷本圭司
    • Organizer
      Keystone Symposia, Hypoxia : Molecular Mechanisms of Oxygen Sensing and Response Pathway
    • Place of Presentation
      Keystone, USA
    • Year and Date
      2010-01-22
  • [Presentation] がんの生物学(教育セミナー)2009

    • Author(s)
      檜山桂子
    • Organizer
      2009年度がん治療認定医教育セミナー
    • Place of Presentation
      幕張
    • Year and Date
      2009-12-19
  • [Presentation] Tumor susceptibility induced by constitutive expression of hypoxia-inducible factor-lalpha in a transgenic mouse model2009

    • Author(s)
      末岡栄三朗
    • Organizer
      第32回日本生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-11
  • [Presentation] GTSE1 : A candidate gene for a new molecular target of pancreatic cancer2009

    • Author(s)
      江口英孝
    • Organizer
      第32回日本生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-11
  • [Presentation] Molecular mechanisms of anti-cancer activity of zoledronate2009

    • Author(s)
      谷本圭司
    • Organizer
      第32回 日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-10
  • [Presentation] Zoledronate inhibits HIF-1α in cancer cells2009

    • Author(s)
      谷本圭司
    • Organizer
      第7回 がんとハイポキシア研究会
    • Place of Presentation
      京都
    • Year and Date
      2009-12-06
  • [Presentation] Tumor development in transgenic mice constitutively expressing hypoxia-inducible factor-1α2009

    • Author(s)
      末岡栄三朗
    • Organizer
      第7回がんとハイポキシア研究会
    • Place of Presentation
      京都
    • Year and Date
      2009-12-06
  • [Presentation] Lung carcinogenesis model : Increase of Malignant potential occurs in transformed bronchial epithelial cells concomitant with TERT upregulation2009

    • Author(s)
      檜山桂子
    • Organizer
      The 14^<th> Congress of the Asian Pacific Society of Respirology
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2009-11-16
  • [Presentation] High telomerase expression without p16/RB aberration in lung cancer cells may be the possible marker of cancer stem cells theoretically and experimentally2009

    • Author(s)
      Arifin M.
    • Organizer
      The 14^<th> Congress of the Asian Pacific Society of Respirology
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2009-11-16
  • [Presentation] Genetic variations in detoxification enzymes and HIF1α in COPD2009

    • Author(s)
      Putra A.C.
    • Organizer
      The 14^<th> Congress of the Asian Pacific Society of Respirology
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2009-11-16
  • [Presentation] Telomerase activation without P16/RB inactivation as a possible marker of stem cell origin in lung adenocarcinoma2009

    • Author(s)
      Arifin M.
    • Organizer
      The 19^<th> HCS-The 3^<rd> Three Universities' Consortium International Symposium "Recent progress in carcinogenesis, progression and therapy of lung cancer"
    • Place of Presentation
      Hiroshima
    • Year and Date
      2009-11-01
  • [Presentation] Possible involvement of HIF-1α function-related polymorphisms in lung carcinogenesis2009

    • Author(s)
      Putra A.C.
    • Organizer
      The 19^<th> HCS-The 3^<rd> Three Universities' Consortium In ternational Symposium "Recent Progress in Carcinogenes is, Progression and Therapy of Lung Cancer"
    • Place of Presentation
      Hiroshima
    • Year and Date
      2009-11-01
  • [Presentation] ゲノム・遺伝子研究と倫理. 教育シンポジウム22009

    • Author(s)
      檜山桂子
    • Organizer
      第47回日本癌治療学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-23
  • [Presentation] 卵巣癌におけるTC(paclitaxel/carboplatin)療法の抗癌剤感受性関連遺伝2009

    • Author(s)
      山本弥寿子
    • Organizer
      第47回日本癌治療学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-22
  • [Presentation] HIF1Aトランスジェニックマウスの腫瘍原性解析2009

    • Author(s)
      谷本圭司
    • Organizer
      博多シンポジウム「内・外環境と生物応答」
    • Place of Presentation
      博多
    • Year and Date
      2009-10-09
  • [Presentation] 食道癌においてインビトロ抗癌剤感受性と臨床的予後に対し逆の相関を示す遺伝子の解析2009

    • Author(s)
      麓祥一
    • Organizer
      第68回日本癌学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-03
  • [Presentation] 癌特異的不死化関連遺伝子GTSE1:膵癌の新たな分子標的侯補2009

    • Author(s)
      江口英孝
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-03
  • [Presentation] Intact p16/RB pathway with high telomerase activity may be the marker of stem-cell origin in lung adenocarcinoma2009

    • Author(s)
      Arifin, M.
    • Organizer
      The 68th Annual Meeting of the Jpn. Cancer Assoc.
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-02
  • [Presentation] A role of polymorphisms of HIF-1α in lung cancer2009

    • Author(s)
      Putra, A.C.
    • Organizer
      The 68th Annual Meeting of the Jpn. Cancer Assoc
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-01
  • [Presentation] トランスジェニックマウスを用いたHIF-1αによる腫瘍原生の解析2009

    • Author(s)
      末岡栄三朗
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-01
  • [Presentation] 癌特異的不死化関連遺伝子SFRS10の選択的スプライシングに関わるネットワークと機能の解析2009

    • Author(s)
      和田智
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-01
  • [Presentation] Exploration of the evidence of cancer stem cells in lung cancer2009

    • Author(s)
      Arifin M.
    • Organizer
      European Respiratory Society Annual Congress 2009
    • Place of Presentation
      Vienna, オーストソア
    • Year and Date
      2009-09-15
  • [Presentation] ゲノム情報に基づく医療の展開2009

    • Author(s)
      檜山桂子
    • Organizer
      日本遺伝看護学会第8回学術大会
    • Place of Presentation
      広島
    • Year and Date
      2009-09-11
  • [Presentation] ビスフォスフォネート製剤の抗腫瘍効果分子機構の解明2009

    • Author(s)
      谷本圭司
    • Organizer
      第10回ホルモンと癌研究会
    • Place of Presentation
      仙台
    • Year and Date
      2009-07-31
  • [Presentation] 新規分子標的候補遺伝子SFRS10の同定と機能解析2009

    • Author(s)
      和田智
    • Organizer
      第13回日本がん分子標的治療学会学術集会
    • Place of Presentation
      徳島
    • Year and Date
      2009-06-26
  • [Presentation] EMP3の臨床的意義とその発現制御機構:食道癌と他がん種の比較2009

    • Author(s)
      麓祥一
    • Organizer
      第63回日本食道学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2009-06-25
  • [Presentation] がん薬物療法個人応答の予測:非仮説マーカーの同定と複数遺伝子による予測の試み2009

    • Author(s)
      Mohamed, A.E.
    • Organizer
      第18回日本癌病態治療研究会
    • Place of Presentation
      福島
    • Year and Date
      2009-06-16
  • [Presentation] Genetic Variations in Telomerase Components and Telomere Shortening in Japanese Patients with COPD2009

    • Author(s)
      Arifin, M.
    • Organizer
      The 49^<th> Annual Meeting of The Japanese Respiratory Society
    • Place of Presentation
      東京
    • Year and Date
      2009-06-13
  • [Presentation] Telomere reset mechanism and efficiency in human stem cells2009

    • Author(s)
      檜山桂子
    • Organizer
      JST-CIRM (California Institute for Regenerative Medicine) Workshop
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2009-06-08
  • [Presentation] DNA修復能に影響を与える生体内環境因子:低酸素環境2009

    • Author(s)
      谷本圭司
    • Organizer
      第50回原子爆弾後障害研究会
    • Place of Presentation
      広島
    • Year and Date
      2009-06-07
  • [Presentation] 道癌における新規癌抑制遺伝子EMP3の同定と放射線感受性因子としての検討2009

    • Author(s)
      麓祥一
    • Organizer
      第50回原子爆弾後障害研究会
    • Place of Presentation
      広島
    • Year and Date
      2009-06-07
  • [Presentation] Randomized phase II/III study of docetaxel/S-1 (DS-1) versus CDDP/5FU (FUP) in advanced or recurrent gastric cancer : Updated phase II results2009

    • Author(s)
      Ikeda R.
    • Organizer
      ASCO 2009 Annual Meeting
    • Place of Presentation
      Orlando, 英国
    • Year and Date
      2009-05-31
  • [Presentation] 保険診療としての遺伝子検査の使い方2009

    • Author(s)
      檜山桂子
    • Organizer
      第二内科開業医会
    • Place of Presentation
      広島
    • Year and Date
      2009-05-11
  • [Presentation] Identification of novel drug-response determinants for 5-FU and CDDP in esophageal squamous cell carcinoma(ESCC)2009

    • Author(s)
      麓祥一
    • Organizer
      Am. Assoc. Cancer Res 100^<th> Annual Meeting 2009
    • Place of Presentation
      Denver, USA
    • Year and Date
      2009-04-22
  • [Presentation] Molecular mechanisms of degradation of the pVHL, tumor suppressor gene product, by a novel oncogenic protein, UBE2S2009

    • Author(s)
      谷本圭司
    • Organizer
      Am. Assoc. Cancer Res 100^<th> Annual Meeting 2009
    • Place of Presentation
      Denver, USA
    • Year and Date
      2009-04-21
  • [Presentation] Possible drug targets screened as key genes in immortalization mechanisms specific for cancer cells2009

    • Author(s)
      和田智
    • Organizer
      Am. Assoc. Cancer Res 100^<th> Annual Meeting 2009
    • Place of Presentation
      Denver, USA
    • Year and Date
      2009-04-21
  • [Presentation] Selection of novel marker genes that predict a response to paclitaxel and carboplatin(TC) therapy in ovarian cancer2009

    • Author(s)
      山本弥寿子
    • Organizer
      Am. Assoc. Cancer Res 100^<th> Annual Meeting 2009
    • Place of Presentation
      Denver, USA
    • Year and Date
      2009-04-19
  • [Book] Diagnostic and prognostic molecular markers in neuroblastoma. in Clinical application of molecular diagnosis -cancer, radiation effects, and human diseases-2010

    • Author(s)
      檜山桂子
    • Total Pages
      220
    • Publisher
      Transworld Research Network
  • [Book] Clinical application of molecular diagnosis -cancer, radiation effects in Clinical application of molecular diagnosis -cancer, radiation effects, and human diseases-2010

    • Author(s)
      檜山桂子
    • Total Pages
      220
    • Publisher
      Transworld Research Network
  • [Book] Individualized chemotherapy for cancer. in Clinical application of molecular diagnosis -cancer, radiation effects, and human diseases-2010

    • Author(s)
      Tanimoto K
    • Total Pages
      220
    • Publisher
      Transworld Research Network
  • [Book] Simple EGFR mutation screening by conventional PCR. In : Clinical application of molecular diagnosis -cancer, radiation effects, and human diseases-2010

    • Author(s)
      Arifin, M
    • Total Pages
      220
    • Publisher
      Transworld Research Network
  • [Book] 細胞死 ; テロメア・テロメラーゼ. In:入門腫瘍内科学2009

    • Author(s)
      檜山桂子
    • Total Pages
      301
    • Publisher
      篠原出版新社
  • [Book] 乳癌とテロメラーゼ. In:みんなに役立つ乳癌の基礎と臨床2009

    • Author(s)
      檜山桂子
    • Total Pages
      1095
    • Publisher
      医薬ジャーナル社

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Published: 2011-06-16   Modified: 2016-04-21  

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