2009 Fiscal Year Final Research Report
A proteomic analysis of sample embedded in paraffin from patients with interstitial lung diseases
| Project/Area Number |
21591005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Tomoko 日本大学, 医学部, 臨床准教授 (10343576)
GON Yasuhiro 日本大学, 医学部, 准教授 (80339316)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 肺線維症 / プロテオミクス / マイクロダイセクション |
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| Research Abstract |
We designed this study to reveal a protein network in diffuse interstitial lung disease by a proteomic analysis of tissue sample, which was selectively collected using laser micro dissection. We modified protein-processing method of paraffin embedded sample prior to mass spectrometer ; however, we could detect only major protein such as keratin and albumin so far, because amount of protein in the dissected sample was extremely small. We need to change our method and use new technology in the future to increase sensitivity, which is enough for a comprehensive analysis of very small sample.
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