2011 Fiscal Year Final Research Report
Development of treatment strategies aimed at nucleocytoplasmic shuttling for small cell lung cancer
| Project/Area Number |
21591013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Aichi Cancer Center Research Institute |
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Principal Investigator |
HORIO Yoshitsugu 愛知県がんセンター(研究所), 分子腫瘍学部, 研究員 (30344336)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 小細胞肺癌 / 核細胞質間シャトル / p27 |
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| Research Abstract |
Cell cycle regulator p27 shuttles between the nucleus and the cytoplasm. Phosphorylation at threonine 198(T198) and 157(T157) increase cell motility. We showed S10 hosphorylation was associated with T198 and T157 phosphorylation in small cell lung cancer(SCLC) cell lines. In 30 surgically resected SCLC specimens, however, immunohistochemistry revealed high expression of phosphorylated p27 at T198 and T157. Leptomycin B or knockdown of cellular CRM1 by siRNA showed additive effects with topoisomerase II inhibitors(Etoposide and Amrubicine) in SCLC cell lines and a fibroblast cell line. There were no correlations between chemosensitivity of amrubicin and mRNA expression levels of the RanBP2, TopoII-alpha and TopoII-beta genes.
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