Structure and function of tight junctions involved in slit diaphragms

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591021
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Niigata University
• Principal Investigator: YAOITA Eishin 新潟大学, 医歯学系, 准教授 (00157950)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Yutaka 新潟大学, 医歯学系, 講師 (40182795) ; YAMOTO Tadashi 新潟大学, 医歯学系, 教授 (30092737)
• Project Period (FY): 2009 – 2011
• Keywords: 腎臓 / 足細胞 / 細胞間結合 / 密着結合 / スリット膜

Research Abstract

Tight junctions are the main intercellular junctions of podocytes of the renal glomerulus under nephrotic conditions. Their requisite components, claudins, still remain to be identified. Absolute quantitative real-time PCR using cDNA of isolated rat glomeruli showed that claudin-5 was the most abundantl claudin expressed in glomeruli. In situ hybridization and immunolocalization studies revealed that claudin-5 was localized mainly in podocytes. Claudin-5 protein was observed on the entire surface of podocytes including slit diaphragms, and was inclined to be concentrated on tight junctions in puromycin aminonucleoside nephrosis. Immunoprecipitation studies using anti-claudin-5 and glomerular lysates revealed the association of claudin-5 and nephrin. It is concluded that claudin-5 is a main claudin expressed in podocytes and that the formation of tight junctions in the nephrosis may be due to local recruitment of claudin-5 rather than due to total upregulation of the claudin protein levels.

Research Products

• [Journal Article] Comparison of human glomerulus proteomic profiles obtained from low quantities of samples by different mass spectrometry with the comprehensive database (2011)
  • Author(s): Zhang Y, Yoshida Y, Xu B, Magdeldin S, Fujinaka H, Liu Z, Miyamoto M, Yaoita E, Yamamoto T
  • Journal Title: Proteome Science Volume: Vol.9, No.1 Pages: 47-57
  • Peer Reviewed
• [Journal Article] Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis (2011)
  • Author(s): Xu B, Zhang Y, Zhao Z, Yoshida Y, Magdeldin S, Fujinaka H, Ismail TA, Yaoita E, Yamamoto T
  • Journal Title: Journal of Proteomics Volume: Vol.74, No.7 Pages: 1022-1029
  • Peer Reviewed
• [Journal Article] Novel expression of claudin-5 in glomerular podocytes (2011)
  • Author(s): Koda R, Zhao L, Yaoita E, Yoshida Y, Tsukita S, Tamura A, Nameta M, Zhang Y, Fujinaka H, Magdeldin S, Xu B, Narita I, Yamamoto T
  • Journal Title: Cell and Tissue Research Volume: Vol.343, No.3 Pages: 637-648
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Sekiguchi S, Suzuki A, Asano S, Nishiwaki-Yasuda K, Shibata M, Nagao S, Yamamoto N, Matsuyama M, Sato Y, Yan K, Yaoita E, Itoh M
  • Journal Title: American Journal of Physiology Renal Physiology Volume: Vol.300, No.4 Pages: 848-856
  • Peer Reviewed
• [Journal Article] Glomerular proteins related to slit diaphragm and matrix adhesion in the foot processes are highly tyrosine phosphorylated in the normal rat kidney (2010)
  • Author(s): Zhang Y, Yoshida Y, Nameta M, Xu B, Taguchi I, Ikeda T, Fujinaka H, Mohamed SM, Tsukaguchi H, Harita Y, Yaoita E, Yamamoto T
  • Journal Title: Nephrology Dialysis Transplantation Volume: Vol.25, No.6 Pages: 1785-1795
  • Peer Reviewed
• [Presentation] 初代培養におけるポドサイトの性質 (2011)
  • Author(s): 矢尾板永信
  • Organizer: 第54回日本腎臓学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-06-11
• [Presentation] 糸球体培養 (2010)
  • Author(s): 矢尾板永信
  • Organizer: 第53回日本腎臓学会学術総会
  • Place of Presentation: 神戸
  • Year and Date: 2010-06-18
• [Presentation] Adherens junction proteins in glomerular podocytes of quail kidney (2010)
  • Author(s): 矢尾板永信
  • Organizer: 2010 Experimental Biology
  • Place of Presentation: Anahein, CA, USA
  • Year and Date: 2010-04-26