Role of glycosylation-related enzymes in cultured podocytes using honeycomb-patterned polymer films

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591022
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Dokkyo Medical University (2010-2011); Niigata University (2009)
• Principal Investigator: TAKEDA Tetsuro 獨協医科大学, 医学部, 教授 (10361924)
• Co-Investigator(Kenkyū-buntansha): SAITO Akihiko 新潟大学, 医歯学総合研究科, 特任教授 (80293207)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Masaru 山形大学, 理工学研究科, 教授 (00322850)
• Project Period (FY): 2009 – 2011
• Keywords: 糸球体上皮細胞 / ハニカム膜 / 慢性腎臓病 / 糖転移酵素

Research Abstract

Glomerular epithelial cells(podocytes) play a key role in the prevention of proteinuria, are important determinants of end-stage kidney disease. Surface charge alterations of the podocyte may induce proteinuric injury. Cultured podocytes are currently being used to uncover unique functions of podocytes that can then be validated in vivo. Therefore, we examined a culture of podocytes on self-organized honeycomb-patterned films(honeycomb films). As a result, podocyte maintained their function for the mRNA expression of unique genes. In addition, we investigated the expression of glycosylation-related enzymes in cultured podocytes. We found that a honeycomb film showed higher podocyte function and angiotensin II stimulation decreased the mRNA level of these enzymes. These results suggest that glycosylation-related enzymes contribute to maintain the negative surface charge of podocytes.

Research Products

• [Journal Article] Significance of Urinary Full-Length and Ectodomain Forms of Megalin in Patients With Type 2 Diabetes (2012)
  • Author(s): Ogasawara S, Hosojima M, Kaseda R, Kabasawa H, Yamamoto-Kabasawa K, Kurosawa H, Sato H, Iino N, Takeda T, Suzuki Y, Narita I, Yamagata K, Tomino Y, Gejyo F, Hirayama Y, Sekine S, Saito A
  • Journal Title: Diabetes Care Volume: 35 Pages: 1112-1118
  • DOI: DOI:10.2337/dc11-1684
  • Peer Reviewed
• [Journal Article] 糸球体病理所見の足細胞のfoot process effacementとは(Q & A) (2011)
  • Author(s): 竹田徹朗
  • Journal Title: 日本医事新報 Volume: 4538 Pages: 52-54
• [Journal Article] Molecular mechanisms of receptor-mediated endocytosis in the renal proximal tubular epithelium (2010)
  • Author(s): Saito A, Sato H, Iino N, and Takeda T
  • Journal Title: Journal of Biomed and Biotechnol Volume: 403272
  • DOI: DOI:10.1155/2010/403272
  • Peer Reviewed
• [Journal Article] Regulation of megalin expression in cultured proximal tubule cells by angiotensin II type 1A receptor-and insulin-mediated signaling cross-talk (2009)
  • Author(s): Hosojima M, Sato H, Yamamoto K, Kaseda R, Soma T, Kobayashi A, Suzuki A, Kabasawa H, Takeyama A, Ikuyama K, Iino N, Nishiyama A, Thekkumkara TJ, Takeda T, Suzuki Y, Gejyo F, and Saito A
  • Journal Title: Endocrinology Volume: 150 Pages: 871-878
  • DOI: DOI:10.1210/en.2008-0886
  • Peer Reviewed
• [Journal Article] Megalin and nonmuscle myosin heavy chain IIA interact with the adaptor protein Disabled-2 in proximal tubule cells (2009)
  • Author(s): Hosaka K, Takeda T, Iino N, Hosojima M, Sato H, Kaseda R, Yamamoto K, Kobayashi A, Gejyo F, and Saito A
  • Journal Title: Kidney Int Volume: 75 Pages: 1308-1315
  • DOI: DOI:10.1038/ki.2009.85
  • Peer Reviewed
• [Journal Article] 尿中podocyte(ポドサイト),尿中ポドカリキシン (2009)
  • Author(s): 竹田徹朗
  • Journal Title: Medical Technology Volume: 37(6) Pages: 564-566
• [Presentation] 生活習慣改善プログラムによる尿中アルブミン排泄量の減少効果 (2011)
  • Author(s): 山本佳子、細島康宏, 蒲澤秀門, 飯野則昭, 竹田徹朗, 鈴木芳樹, 成田一衛, 荒川正昭, 斎藤亮彦
  • Organizer: 第54回日本腎臓学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 20110615-17
• [Presentation] Low calorie diet with formula food reduces albuminuria in proportion to the improvement in fractional excretion of uric acid in obese men (2010)
  • Author(s): T Takeda, K Yamamoto, N Iino, I Narita, A Saito
  • Organizer: ISN-Nexus Symposium 2010 : The Kidney and the Vascular System
  • Place of Presentation: Kyoto
  • Year and Date: 20100415-18
• [Presentation] Low calorie diet reduces albuminuria in proportion to the improvement in serum leptin level and fractional excretion of uric acid in obese men (2009)
  • Author(s): Tetsuro Takeda, Keiko Yamamoto, Noriaki Iino, Ichiei Narita, Akihiko Saito
  • Organizer: American Society of Nephrology Renal Week 2009
  • Place of Presentation: San Diego, California, USA
  • Year and Date: 20091027-1101
• [Presentation] 肥満者に対するフォーミュラ食を用いた減量プログラムによるアルブミン尿の減少効果 (2009)
  • Author(s): 竹田徹朗, 山本佳子, 飯野則昭, 斎藤亮彦, 鈴木芳樹, 下条文武
  • Organizer: 第106回日本内科学会総会・講演会
  • Place of Presentation: 東京
  • Year and Date: 20090410-12
• [Presentation] Effects of lifestyle modification with combined aerobic and resistance exercise on urinary albumin excretion in the population with metabolic syndrome (2009)
  • Author(s): Keiko Yamamoto, Michihiro Hosojima, Mariko Saito, Junta Tanaka, Masao Hayashi, Noriaki Iino, Tetsuro Takeda, Yoshiki Suzuki, Fumitake Gejyo, Masaaki Arakawa, Akihiko Saito
  • Organizer: 3rd International Congress on Prediabetes and the Metabolic Syndrome
  • Place of Presentation: Nice, France
  • Year and Date: 20090401-04
• [Book] 腎臓症候群上 (2012)
  • Author(s): 川本進也、竹田徹朗
  • Total Pages: 703-709
  • Publisher: 日本臨床社