Isolation of erythropoietin-producing cells using embryonic stem cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591037
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Nara Medical University
• Principal Investigator: KUBO Atsushi 奈良県立医科大学, 医学部, 助教 (30316062)
• Co-Investigator(Kenkyū-buntansha): IWANO Masayuki 奈良県立医科大学, 医学部, 講師 (20275324)
• Project Period (FY): 2009 – 2011
• Keywords: ES細胞 / エリスロポエチン / 腎臓

Research Abstract

1. Targeting vector was designed to integrate EGFP in erythropoietin(EPO) gene. EGFP-IRES-puroR gene was knocked-in into the start codon of EPO gene. This vector was electroprated into C57BL/6 derived ES cells and then EPO-EGFP ES cells were established. These ES cells were cultured in various condition such as ectoderm, mesoderm and endoderm and EGFP was evaluated by FACS.
2. EPO-EGFP ES cells were injected into mouse blastcyst and then EPO-EGFP+/-mouse was established. Section of kidney from EPO-EGFP+/-was analyzed by fluorescent microscopy. Isolated cells from EPO-EGFP+/-mouse were analyzed by FACS.

Research Products

• [Journal Article] Pdx1 and Ngn3 overexpression enhances pancreatic differentiation of mouse ES cell-derived endoderm population (2011)
  • Author(s): Kubo A, Stull R, Takeuchi M, Bonham K, Gouon-Evans V, Sho M, Iwano M, Saito Y, Keller G, Snodgrass R
  • Journal Title: PLoS One Volume: 6 Pages: e2405
• [Journal Article] The Homeobox Gene Hex regulates hepatocyte differentiation from embryonic stem cell-derived endoderm (2010)
  • Author(s): Kubo A, Kim YH, Irion S, Kasuda S, Takeuchi M, Ohashi K, Iwano M, Dohi Y, Saito Y, Snodgrass R, Keller G
  • Journal Title: Hepatology Volume: 51 Pages: 633-641
• [Journal Article] Possible involvement of stem-like populations with elevated ALDH1 in sarcomas for chemotherapeutic drug resistance (2010)
  • Author(s): Honoki K, Fujii H, Kubo A, Kido A, Mori T, Tanaka Y, Tsujiuchi T
  • Journal Title: Oncol Rep Volume: 24(2) Pages: 501-5
• [Journal Article] Epithelial-mesenchymal transition as a potential explanation for podocyte depletion in diabetic nephropathy (2009)
  • Author(s): Yamaguchi Y, Iwano M, Suzuki D, Nakatani K, Kimura K, Harada K, Kubo A, Akai Y, Toyoda M, Kanauchi M, Neilson EG, Saito Y
  • Journal Title: Am J Kidney Dis Volume: 54(4) Pages: 653-64
• [Journal Article] Treatment with recombinant placental growth factor(PlGF) enhances both angiogenesis and arteriogenesis and improves survival after myocardial infarction (2009)
  • Author(s): Takeda Y, Uemura S, Iwama H, Imagawa K, Nishida T, Onoue K, Takemoto Y, Soeda T, Okayama S, Somekawa S, Ishigami K, Takaoka M, Kawata H, Kubo A, Horii M, Nakajima T, Saito Y
  • Journal Title: Circ J Volume: 73(9) Pages: 1674-82
• [Presentation] The Homebox Gene HEX Regulates Hepatocyte Differentiation from ES cell-Derived Endoderm (2010)
  • Author(s): Kubo A, Takeuchi M, Kasuda S, Iwano M, Snodgrass R, Keiller G, Saito Y
  • Organizer: International Symposium on Cardiovascular Endocrinology and Metabolism(CVEM)
  • Place of Presentation: Nara, Japan
  • Year and Date: 20100331-0401
• [Presentation] Stable expression of HIF-1αin tubular epithelial cells promotes interstitial fibrosis (2010)
  • Author(s): Kimura K, Iwano M, Yamaguchi Y, Nakatani K, Kubo A, Akai Y, Saito Y
  • Organizer: International Symposium on Cardiovascular Endocrinology and Metabolism(CVEM)
  • Place of Presentation: Nara, Japan
  • Year and Date: 20100331-0401
• [Presentation] Epithelial-mesenchymal transition as an explanation for podocyte depletion in diabetic nephropathy (2010)
  • Author(s): Yamaguchi Y, Iwano M, Suzuki D, Nakatani K, Kimura K, Harada K, Kubo A, Akai Y, Toyoda M, Kanauchi M, Neilson G E, Saito Y
  • Organizer: International Symposium on Cardiovascular Endocrinology and Metabolism(CVEM)
  • Place of Presentation: Nara, Japan
  • Year and Date: 20100331-0401