2011 Fiscal Year Final Research Report

Identification of quantitative trait loci for diabetic nephropathy in KK-Ay/Ta mice

Research Project

Project/Area Number	21591039
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Kidney internal medicine
Research Institution	Juntendo University
Principal Investigator	TOMINO Yasuhiko 順天堂大学, 医学部, 教授 (60130077)
Co-Investigator(Kenkyū-buntansha)	GODA Tomohito 順天堂大学, 医学部, 助教 (20365604) TSNIMOTO Mituo 順天堂大学, 医学部, 助教 (00420836) HAGIWARA Sinji 順天堂大学, 医学部, 助教 (70445568)
Project Period (FY)	2009 – 2011
Keywords	糖尿病腎症 / KK-Ay/Taマウス / QTL / ACR / HbA1c
Research Abstract	We studied candidate gene loci for diabetic nephropathy using quantitative trait locus(QTL) analysis of KK-Ay/Ta×BALB/cA F2 intercross mice. We examined 270(KK-Ay/Ta×BALB/cA) F2 intercross mice. Genotypes were investigated using microsatellite markers with QTL analysis. ACR in mice at 20 weeks and ACR gain showed a suggestive linkage to chromosome 9. Gene loci contributing to HbA1c indicated a significant linkage to chromosome 7 at 8 and 12 weeks(designed Fbw-1). QTL analysis of KK-Ay/Ta mice revealed several new loci contributing to diabetic nephropathy and related phenotypes. Thus, it appears that type 2 diabetes and nephropathy of KK-Ay/Ta mice have different genetic factors.