The development for new peritoneal dialysate to reduce oxidative stress and apoptosis using rare sugar

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591055
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Tohoku University (2010-2011); Kagawa University (2009)
• Principal Investigator: KIYOMOTO Hideyasu 東北大学, 東北メディカル・メガバンク機構, 教授 (00304585)
• Project Period (FY): 2009 – 2011
• Keywords: 腹膜中皮細胞 / 希少糖 / D-プシコース / 酸化ストレス / 慢性腎不全 / 腹膜透析 / NADPHオキシターゼ / アポトーシス

Research Abstract

In order to clarify the protective fluid in peritoneal dialysis(PD), we investigated the effects of psicose, one of rare sugars, on peritoneal mesothelial cells(PMCs)injury induced by D-glucose. ROS production was significantly increased in PMCs treated with 83mM D-glucose containing medium. Both D-and L-psicose prevented the D-glucose induced ROS production via suppression of p22 phox, a NADPH oxidase membrane component. These results suggested that decrease of ROS production was possible mechanisms that psicose suppressed peritoneal deterioration. Then, we are focus the medical relevance in vivo animal models. We conclude that psicose containing dialysate may be beneficial effect on PMCs survival in PD therapy ; however it will be necessary to proof the concept in vivo animals.

Research Products

• [Journal Article] Early treatment with olmesartan prevents juxtamedullary glomerular podocyte injury and the onset of microalbuminuria in type 2 diabetic rats (2012)
  • Author(s): Sofue T, Kiyomoto H, Kobori H, Urushihara M, Nishijima Y, Kaifu K, Hara T, Matsumoto S, Ichimura A, Ohsaki H, Hitomi H, Kawachi H, Hayden MR, Whaley-Connell A, Sowers JR, Ito S, Kohno M, Nishiyama A.
  • Journal Title: Am J Hypertens Volume: 25(5) Pages: 604-11
  • Peer Reviewed
• [Journal Article] New era for drug discovery and development in renal disease (2011)
  • Author(s): Miyata T, Kikuchi K, Kiyomoto H, van Ypersele de Strihou C
  • Journal Title: Nature Review Nephrology Volume: 7 Pages: 469-477
  • Peer Reviewed
• [Journal Article] Angiotensin II shifts insulin signaling into vascular remodeling from glucose metabolism in vascular smooth muscle cells (2011)
  • Author(s): Hitomi H, Kaifu K, Fujita Y, Sofue T, Nakano D, Moriwaki K, Hara T, Kiyomoto H, Kohno M, Kobori H, Nishiyama A.
  • Journal Title: Am J Hypertens Volume: 24(10) Pages: 1149-55
  • Peer Reviewed
• [Journal Article] Chronic block-ade of the (pro)renin receptor amelio-rates the kidney damages in non-clipped kidney of Goldblatt hypertension (2011)
  • Author(s): Kiyomoto H, Moriwaki K
  • Journal Title: Hypertens Res Volume: 34 Pages: 289-291
  • Peer Reviewed
• [Journal Article] 急性腎障害(AKI)の診療. 保存的治療(薬物療法などを含む) (2010)
  • Author(s): 清元秀泰、森脇久美子
  • Journal Title: Modern Physician Volume: 31 Pages: 95-99
• [Journal Article] Regression ofsuperficial glomerular podocyte injury in type 2 diabetic rats with overt albuminuria : effect of angiotensin II blockade (2010)
  • Author(s): Ihara G, Kiyomoto H, Kobori H, Nagai Y, Ohashi N, Hitomi H, Nakano D, Pelisch N, Hara T, Mori T, Ito S, Kohno M, Nishiyama A.
  • Journal Title: J Hypertens Volume: 28 Pages: 2289-98
  • Peer Reviewed
• [Journal Article] Insulin attenuates apoptosis induced by high glucose via the PI3-kinase/Akt pathway in rat peritoneal mesothelial cells (2010)
  • Author(s): Kaifu K, Kiyomoto H, Hitomi H, Matsubara K, Hara T, Moriwaki K, Ihara G, Fujita Y, Sugasawa S, Nagata D, Nishiyama A, Kohno M.
  • Journal Title: Nephrol Dial Transplant Volume: 24(3) Pages: 809-815
  • Peer Reviewed
• [Journal Article] Involvement of mineralocorticoid receptor in high glucose-induced big mitogen-activated protein kinase 1 activation and mesangial cell proliferation (2010)
  • Author(s): Liu G, Miyata K, Hitomi H, Yao L, Sun GP, Suzaki Y, Hosomi N, Kiyomoto H, Nakano D, Tamaki T, Yoshizumi M, Nishiyama A.
  • Journal Title: J Hypertens Volume: 28(3) Pages: 536-42
  • Peer Reviewed
• [Presentation] The remedial effect of strict diet treatment and exercise therapy using information technology (IT)on metabolic syndrome (2010)
  • Author(s): Kiyomoto H, et. al
  • Organizer: 43rd Annual meeting of American Society of Nephrology, Renal Week.
  • Place of Presentation: Denver, CO, USA
  • Year and Date: 2010-10-23
• [Presentation] Psicose containing solution suppresses glucose-induced reactive oxygen species (ROS)dependent epithelial to mesenchymal transition (EMT)in cultured rat peritoneal mesothelial cells (2009)
  • Author(s): Kiyomoto H, 他
  • Organizer: American Society of Nephrology(アメリカ腎臓学会)
  • Place of Presentation: サンディエゴ国際コンベンションセンター(アメリカ)
  • Year and Date: 2009-11-03
• [Presentation] The inhibitory effect of rare sugars on epithelial-mesenchymal transition (EMT)induced by high concentration of D-glucose in cultured rat peritoneal mesothelial cells (2009)
  • Author(s): Kiyomoto H, 他
  • Organizer: World Congress of Nephrology(国際腎臓会議)
  • Place of Presentation: ミラノ国際コンベンションセンター(イタリア)
  • Year and Date: 2009-06-02
• [Book] Renin angiotensin system in the kidney and oxidative stress-Local Renin Angiotensin Aldosterone System (RAAS)and NAD(P)H oxidase-dependent oxidative stress in the kidney-. Oxidative Stress in Basic Research and Clinical Practice : Renal Disorders (2010)
  • Author(s): Kiyomoto H, Kobori H, Nishiyama A.
  • Total Pages: 71-91
  • Publisher: Springer, Humana Press, Swiss