2012 Fiscal Year Final Research Report
精神機能に及ぼす甲状腺ホルモン作用におけるRCAN/カルシニューリン系の役割
| Project/Area Number |
21591173
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Nagoya University |
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Principal Investigator |
KANOU Yasuhiko 名古屋大学, 環境医学研究所, 助教 (50252292)
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| Co-Investigator(Kenkyū-buntansha) |
MIZOGUCHI Hiroyuki 名古屋大学, 環境医学研究所, 助教 (70402568)
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| Project Period (FY) |
2009 – 2012
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| Keywords | 内分泌学 / 神経科学 / 脳・神経 / 遺伝子 / タンパク質 |
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| Research Abstract |
RCAN (regulators of calcineurin) 2 is induced with a thyroxin (T3) and it controls the activity of a calcineurin (CaN). CaN is a protein phosphatase which exists in a CNS at a rich, and it is reported that unusuallies arise in a learning and a memory, or a mentation by the knock out and overexpression. Since the expression of cerebral RCAN2 reduced due to the hypothyroidism, RCAN2 participated in the cerebral function deeply and we assumed that the effect to the cerebral function of a thyroxin was demonstrated through the regulation of gene expression of RCAN2. This study examined the role in the cerebral function of RCAN2 protein through the morphological and behavioral analyses of the previously established RCAN2 genetically engineered mouse (knock out mouse). A RCAN2 KO mice is born normally and the abnormality compared with a wild type was not observed. We established as a congenic strain to C57BL/6J line, which carried out the back, cross, and made the backcross generation's 10 to
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11 (N10or11) F2 mice, and provided the behavioral analyze. Moreover, we deposited the mice to the Riken bioresource center. In a nascent, a growth period, and an adult, a morphological variation does not have a RCAN2KO mice in a brain compared with a wild type mice, and a future still more detailed investigation is called for. As a result of measuring the amount of behaviourals of a RCAN2 KO mice, the difference [superiority / the amount of spontaneous behaviourals of KO mice, a wild type mice, and a hetero type mice] was not accepted. However, the amount of behavioral of KO mice was decreasing immediately after moving to a novel situation (novel cage for a measurement). Furthermore, I considered the difference to be an out-of thing to the emotional behavior and learning ability that have been measured by other behavioral trials (an elevated type crux maze trial, Y character maze trial, a forced-swimming trial, phobia conditional learning tests). The analyze by the "Japan mouse clinic" which a Riken bioresource center carry out is under perform. Less
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[Journal Article] Chronic restraint stress impairs neurogenesis and hippocampus-dependent fear memory in mice: possible involvement of a brain-specific transcription factor Npas42011
Author(s)
Yun J, Koike H, Ibi D, Toth E, Mizoguchi H, Nitta A, Yoneyama M, Ogita K, Yoneda Y, Nabeshima T, Nagai T, Yamada K.
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Journal Title
Journal of Neurochemistry
Volume: Vol. 30
Pages: 5702-5712
Peer Reviewed
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[Journal Article] A Novel Caspr Mutation Causes the Shambling Mouse Phenotype by Disrupting Axoglial Interactions of Myelinated Nerves.2009
Author(s)
Sun XY, Takagishi Y, Okabe E, Chishima Y, Kanou Y, Murase S, Mizumura K, Inaba M, Komatsu Y, Hayashi Y, Peles E, Oda SI, Murata Y.
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Journal Title
Journal of Neuropathology and Experimental Neurology
Volume: Vol. 68
Pages: 1207-1218
Peer Reviewed
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[Journal Article] Matrix metalloprotease-9 inhibition improves amyloid β-mediated cognitive impairment and neurotoxicity in mice.2009
Author(s)
Mizoguchi H, Takuma K, Fukuzaki E, Ibi D, Someya E, Akazawa K, Alkam T, Tsunekawa H, Mouri A, Noda Y, Nabeshima T, and Yamada K.
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Journal Title
Journal of Pharmacology and Experimental Therapeutics.
Volume: Vol. 331
Pages: 14-22
Peer Reviewed
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