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2011 Fiscal Year Final Research Report

Characterization of human CD34-negative hematopoietic stem cells and their application for regenerative medicine

Research Project

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Project/Area Number 21591251
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionKansai Medical University

Principal Investigator

SONODA Yoshiaki  関西医科大学, 医学部, 教授 (60206688)

Co-Investigator(Renkei-kenkyūsha) SASAKI Yutaka  関西医科大学, 医学部, 講師 (80425066)
UEMURA Yasushi  関西医科大学, 医学部, 講師 (40364781)
MATSUOKA Yoshikazu  関西医科大学, 医学部, 助教 (70533420)
Project Period (FY) 2009 – 2011
Keywords造血幹細胞 / 臍帯血 / CD34抗原 / 骨髄内移植法 / 重症免疫不全マウス / 再生医療
Research Abstract

We have identified human cord blood-derived very primitive CD34-negative hematopoietic stem cell(CD34-HSC) using the intra-bone marrow injection technique for the first time. In addition, we developed a high resolution purification method for CD34-HSC using 18 lineage-specific antibodies and succeeded to identify positive markers of CD34-HSC. Furthermore, we demonstrated that the CD34-HSC seemed to be more immature than the CD34+CD38-HSC. Based on these data, we propose a novel model of human HSC hierarchy.

  • Research Products

    (40 results)

All 2012 2011 2010 2009 Other

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (31 results) Remarks (1 results)

  • [Journal Article] The low level of c-kit expression marks deeply quiescent murine hematopoietic stem cells2011

    • Author(s)
      Matsuoka Y, Sasaki Y, Nakatsuka R, Takahashi M, Iwaki R, Uemura Y, Sonoda Y
    • Journal Title

      Stem Cells

      Volume: 29 Pages: 1783-1791

    • Peer Reviewed
  • [Journal Article] CXCL8 enhances the angiogenic activity of umbilical cord blood-derived outgrowth endothelial cells in vitro2011

    • Author(s)
      Kimura T, Kohno H, Matsuoka Y, Murakami M, Nakatsuka R, Hase H, Yasuda K, Uemura Y, Sasaki Y, Fukuhara S, Sonoda Y
    • Journal Title

      Cell Biol Int

      Volume: 35 Pages: 201-208

    • Peer Reviewed
  • [Journal Article] Development of a high resolution purification method for precise functional characterization of primitive human cord blood-derived CD34-negative SCID-repopulating cells2011

    • Author(s)
      Ishii M, Matsuoka Y, Sasaki Y, Nakatsuka R, Takahashi M, Nakamoto T, Yasuda K, Matsui K, Asano H, Uemura Y, Fukuhara S, Sonoda Y
    • Journal Title

      Exp Hematol

      Volume: 39 Pages: 203-213

    • Peer Reviewed
  • [Journal Article] CD16 antigen is a positive marker of peripheral blood-derived early endothelial progenitor cells2011

    • Author(s)
      Kimura T, Kohno H, Matsuoka Y, Nakatsuka R, Sasaki Y, Fukuhara S, Sonoda Y
    • Journal Title

      Int J Hematol

      Volume: 93 Pages: 123-125

    • Peer Reviewed
  • [Journal Article] 5-Aza-2'-deoxycytidine treatment induces skeletal myogenic differentiation of mouse dental pulp stem cells2010

    • Author(s)
      Nakatsuka R, Nozaki T, Uemura Y, Matsuoka Y, Sasaki Y, Shinohara M, Ohura K, Sonoda Y
    • Journal Title

      Arch Oral Biol

      Volume: 55 Pages: 350-357

    • Peer Reviewed
  • [Journal Article] ヒト未分化CD34抗原陰性造血幹細胞の純化とその幹細胞特性2010

    • Author(s)
      薗田精昭
    • Journal Title

      血液・腫瘍科

      Volume: 61 Pages: 224-232

  • [Journal Article] In vivo dynamics of human cord blood-derived CD34-SCID-repopulating cells using intra-bone marrow injection2010

    • Author(s)
      Kimura T, Matsuoka Y, Murakami M, Kimura T, Takahashi M, Nakamoto T, Yasuda K, Matsui K, Kobayashi K, Imai S, Asano H, Nakatsuka R, Uemura Y, Sasaki Y, Sonoda Y
    • Journal Title

      Leukemia

      Volume: 24 Pages: 162-168

    • Peer Reviewed
  • [Journal Article] Marginal expression of CXCR4 on c-kit+Sca-1+Lineage-hematopoietic stem/progenitor cells2009

    • Author(s)
      Sasaki Y, Matsuoka Y, Hase M, Toyohara T, Murakami M, Takahashi M, Nakatsuka R, Uemura Y, Sonoda Y
    • Journal Title

      Int J Hematol

      Volume: 90 Pages: 553-560

    • Peer Reviewed
  • [Presentation] マウス組織に由来する間葉系幹細胞と歯髄幹細胞機能比較~ヒト造血幹細胞のin vitro支持能に関して2012

    • Author(s)
      中塚隆介、松岡由和、岩城隆二、高橋雅也、佐々木豊、薗田精昭
    • Organizer
      第34回日本造血細胞移植学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2012-02-25
  • [Presentation] 造血幹細胞とニッチ2012

    • Author(s)
      佐々木豊
    • Organizer
      第34回日本造血細胞移植学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2012-02-25
  • [Presentation] A novel model of human hematopoietic stem cell hierarchy2012

    • Author(s)
      Sonoda Y
    • Organizer
      The 34th Annual Meeting of the Japan Society for Hematopoietic Cell Transplantation. Presidential Symposium
    • Place of Presentation
      Osaka
    • Year and Date
      2012-02-24
  • [Presentation] ヒト造血幹細胞支持能を有するヒト骨髄由来間葉系幹細胞の予期的分離2012

    • Author(s)
      松岡由和、高橋雅也、岩城隆二、佐々木豊、中塚隆介、河野比良夫、植村靖史、井上雅美、小川啓恭、高橋隆幸、石川淳、日野雅之、薗田精昭
    • Organizer
      第34回日本サイトメトリー学会ワークショップ
    • Place of Presentation
      大阪
    • Year and Date
      2012-02-24
  • [Presentation] マウス切歯由来歯髄幹細胞によるヒト造血幹細胞のin vitro支持能の検討2011

    • Author(s)
      中塚隆介、松岡由和、岩城隆二、高橋雅也、佐々木豊、薗田精昭
    • Organizer
      第73回日本血液学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-10-14
  • [Presentation] 新しいヒト造血幹細胞の分化経路・階級性の提唱2011

    • Author(s)
      薗田精昭
    • Organizer
      第64回大阪血液疾患談話会
    • Place of Presentation
      大阪
    • Year and Date
      2011-07-02
  • [Presentation] マウス骨髄造血幹細胞分画におけるc-kit発現レベルの検討2011

    • Author(s)
      佐々木豊、松岡由和、中塚隆介、高橋雅也、岩城隆二、植村靖史、薗田精昭
    • Organizer
      第21回日本サイトメトリー学会
    • Place of Presentation
      京都
    • Year and Date
      2011-06-26
  • [Presentation] 歯形成端に存在する新規マウス歯髄幹細胞の予期的分離と特性解明2011

    • Author(s)
      中塚隆介、松岡由和、岩城隆二、高橋雅也、佐々木豊、薗田精昭
    • Organizer
      第21回日本サイトメトリー学会
    • Place of Presentation
      京都
    • Year and Date
      2011-06-26
  • [Presentation] ヒト造血幹細胞支持能を有するヒト骨髄由来間葉系幹細胞の予期的分離2011

    • Author(s)
      松岡由和、佐々木豊、薗田精昭
    • Organizer
      第21回日本サイトメトリー学会会長シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2011-06-25
  • [Presentation] Identification and functional characterization of human primitive CD34-negative hematopoietic stem cells : Proposal of novel model of human hematopoietic cell hierarchy2011

    • Author(s)
      Sonoda Y
    • Organizer
      1st Baltic Stem Cell Meeting
    • Place of Presentation
      Szczecin, Poland
    • Year and Date
      2011-05-28
  • [Presentation] Hypoxic culture condition facilitate the proliferation and differentiation potentials of mouse dental pulp stem cells2011

    • Author(s)
      Nakatsuka R, Matsuoka Y, Takahashi M, Iwaki R, Sasaki Y, Sonoda Y
    • Organizer
      The 9th Stem Cell Research Symposium
    • Place of Presentation
      Tokyo
    • Year and Date
      2011-05-14
  • [Presentation] Dynamics of the expression of CXCR4 on murine hematopoietic stem/progenitor cells2011

    • Author(s)
      Sasaki Y, Matsuoka Y, Takahashi M, Iwaki R, Nakatsuka R, Sonoda Y
    • Organizer
      The 9th Stem Cell Research Symposium
    • Place of Presentation
      Tokyo
    • Year and Date
      2011-05-14
  • [Presentation] 未分化造血幹細胞の支持能を持つヒト骨髄由来間葉系幹細胞の予期的分離とその機能解析2011

    • Author(s)
      松岡由和、佐々木豊、高橋雅也、岩城隆二、中塚隆介、井上雅美、小川啓恭、高橋隆幸、石川淳、日野雅之、薗田精昭
    • Organizer
      第33回日本造血細胞移植学会
    • Place of Presentation
      松山
    • Year and Date
      2011-03-10
  • [Presentation] ヒト造血幹細胞の本体はどこまで解明されたのか2011

    • Author(s)
      薗田精昭
    • Organizer
      第6回近畿血液疾患治療研究会
    • Place of Presentation
      京都
    • Year and Date
      2011-01-22
  • [Presentation] Prospective isolation of mouse Sca-1+PDGFR□+dental pulp stem cells(DPSCs) existing in the tooth forming niche2010

    • Author(s)
      Nakatsuka R, Uemura Y, Matsuoka Y, Sasaki Y, Sonoda Y
    • Organizer
      The 8th Stem Cell Research Symposium
    • Place of Presentation
      Awaji
    • Year and Date
      20100513-15
  • [Presentation] Prospective isolation and functional characterization of human bone marrow-derived hematopoietic stem cell-supportive mesenchymal stromal cells2010

    • Author(s)
      Sonoda Y
    • Organizer
      Hematology Conference
    • Place of Presentation
      James Graham Brown Cancer Center, University of Louisville, Louisville, U. S. A.
    • Year and Date
      2010-12-10
  • [Presentation] Prospective isolation and functional characterization of human bone marrow-derived hematopoietic stem cell-supportive mesenchymal stromal cells2010

    • Author(s)
      Matusoka Y, Sasaki Y, Takahashi M, Nakatsuka R, Uemura Y, Inoue M, Ogawa H, Takahashi T, Ishikawa J, Hino M, Sonoda Y
    • Organizer
      The 52nd Annual Meeting of American Society of Hematology
    • Place of Presentation
      Orland, U. S. A
    • Year and Date
      2010-12-07
  • [Presentation] Characterization of murine hematopoietic stem cell compartment based on the c-kit expression level2010

    • Author(s)
      Sasaki Y, Matusoka Y, Nakatsuka R, Uemura Y, Sonoda Y
    • Organizer
      第72回日本血液学会
    • Place of Presentation
      横浜
    • Year and Date
      2010-09-25
  • [Presentation] Prospective isolation and functional characterization of human bone marrow-derived MSCs2010

    • Author(s)
      Matusoka Y, Sasaki Y, Takahashi M, Nakatsuka R, Inoue M, Ogawa H, Takahashi T, Ishikawa J, Hino M, Uemura Y, Sonoda Y
    • Organizer
      第72回日本血液学会
    • Place of Presentation
      横浜
    • Year and Date
      2010-09-24
  • [Presentation] ヒト未分化CD34抗原陰性造血幹細胞の高度純化と特性解明2010

    • Author(s)
      佐々木豊、石井真理、松岡由和、薗田精昭
    • Organizer
      第20回日本サイトメトリー学会
    • Place of Presentation
      東京
    • Year and Date
      2010-06-26
  • [Presentation] Prospective isolation and functional characterization of human hematopoietic stem cell-supportive mesenchymal stromal cells2010

    • Author(s)
      Matsuoka Y, Sasaki Y, Takahashi M, Nakatsuka R, Uemura Y, Sonoda Y
    • Organizer
      The 8th Stem Cell Research Symposium
    • Place of Presentation
      Awaji
    • Year and Date
      2010-05-13
  • [Presentation] High resolution purification and functional characterization of primitive human cord blood-derived CD34-negative SRCs2010

    • Author(s)
      Sasaki Y, Murakami M, Matsuoka Y, Takahashi M, Nakatsuka R, Uemura Y, Sonoda Y
    • Organizer
      The 8th Stem Cell Research Symposium
    • Place of Presentation
      Awaji
    • Year and Date
      2010-05-13
  • [Presentation] Sca-1陽性PDGFRα陽性マウス歯髄幹細胞の予期的分離と特性の解析2010

    • Author(s)
      中塚隆介、松岡由和、岩城隆二、高橋雅也、佐々木豊、薗田精昭
    • Organizer
      第9回日本再生医療学会
    • Place of Presentation
      広島
    • Year and Date
      2010-03-19
  • [Presentation] ヒト臍帯血由来未分化CD34抗原陰性造血幹細胞の純化とその特性解明2010

    • Author(s)
      村上真理、松岡由和、中塚隆介、高橋雅也、植村靖史、佐々木豊、福原資郎、薗田精昭
    • Organizer
      第32回日本造血細胞移植学会
    • Place of Presentation
      浜松
    • Year and Date
      2010-02-19
  • [Presentation] Isolation of mouse dental pulp-derived Sca-1+PDGFR□+tissue-committed stem cells2009

    • Author(s)
      Nakatsuka R, Matsuoka Y, Uemura Y, Sasaki Y, Sonoda Y
    • Organizer
      The 7th Stem Cell Research Symposium
    • Place of Presentation
      Tokyo
    • Year and Date
      20090515-16
  • [Presentation] Kinetics of CXCR4 expression on murine steady state KSL cells2009

    • Author(s)
      Sasaki Y, Matsuoka Y, Toyohara T, Hase M, Nakatsuka R, Uemura Y, Sonoda Y
    • Organizer
      The 7th Stem Cell Research Symposium
    • Place of Presentation
      Tokyo
    • Year and Date
      20090515-16
  • [Presentation] High resolution purification and characterization of human cord blood-derived CD34-negative SCID-repopulating cells with a very immature phenotype2009

    • Author(s)
      Murakami M, Matusoka Y, Nakatsuka R, Takahashi M, Nakamoto T, Yasuda K, Matsui K, Uemura Y, Sasaki Y, Tsuji T, Fukuhara S, Sonoda Y
    • Organizer
      The 51st Annual Meeting of American Society of Hematology
    • Place of Presentation
      New Orleans, U. S. A
    • Year and Date
      2009-12-07
  • [Presentation] マウス骨髄造血幹/前駆細胞はCXCR4低発現である2009

    • Author(s)
      佐々木豊、松岡由和、豊原貴之、長谷真、中塚隆介、植村靖史、薗田精昭
    • Organizer
      第71回日本血液学会
    • Place of Presentation
      京都
    • Year and Date
      2009-10-25
  • [Presentation] マウス骨髄由来Lineage-Sca-1+c-kitlow/-細胞の造血幹(前駆)細胞特性の解明2009

    • Author(s)
      松岡由和、佐々木豊、中塚隆介、植村靖史、薗田精昭
    • Organizer
      第71回日本血液学会
    • Place of Presentation
      京都
    • Year and Date
      2009-10-25
  • [Presentation] ヒト未分化CD34抗原陰性造血幹細胞の純化とその特性解明2009

    • Author(s)
      村上真理、松岡由和、中塚隆介、植村靖史、佐々木豊、福原資郎、薗田精昭
    • Organizer
      第71回日本血液学会
    • Place of Presentation
      京都
    • Year and Date
      2009-10-23
  • [Presentation] In vivo dynamics of human cord blood-derived CD34-negative SCID-repopulating cells(SRCs) in comparison to CD34+CD38+and CD34+CD38-SRCs using intra-bone marrow injection2009

    • Author(s)
      Sonoda Y, Kimura T, Matusoka Y, Murakami M, Nakamoto T, Yasuda K, Nakatsuka R, Uemura Y, Sasaki Y
    • Organizer
      TERMIS 2nd World Congress
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2009-09-02
  • [Remarks] 幹細胞生物学講座(衛生学講座)

    • URL

      http://WWW3.kmu.ac.jp/hygiene/index.html

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Published: 2013-07-31  

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