2013 Fiscal Year Final Research Report
Mechanism of systemic lupus erythematosus accompanied with the aberrant TCR zeta mRNA 3'UTR
| Project/Area Number |
21591268
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Project Period (FY) |
2009-04-01 – 2014-03-31
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| Keywords | TCR zeta / SLE / knockout mouse / 3'UTR |
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| Research Abstract |
To consider whether 3'UTR CS1 region prescribing stability of T-cell receptor zeta chain (TCR zeta) mRNA is associated with TCR zeta chain expression control, We made arm which is lack of CS1 and built a targeting vector. We introduced this targeting vector into an embryonic stem cell successively and made a recombinant embryonic stem cell. Furthermore, we injected it by the injection method and performed the manufacturing of chimera mouse. We crossbred this chimera mouse and wild-type mouse, and an F1 mouse was obtained. However, we tried the mating with the Cre mouse, but did not work well to remove pgk-neo cassette sandwiched in loxP incorporated in the genome of the F1 mouse.
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[Journal Article] Regulatory mechanisms for the production of BAFF and IL-6 are impaired in monocytes of patients of primary Sjögren's syndrome2011
Author(s)
Yoshimoto K, Tanaka M, Kojima M, Setoyama Y, Kameda H, Suzuki K, Tsuzaka K, Ogawa Y, Tsubota K, Abe T, Takeuchi T
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Journal Title
Arthritis Res Ther
Volume: 13(5)(Epub)
Pages: R170
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