Establishment of diagnosis of influenza C virus infection

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591380
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Yamagata University
• Principal Investigator: MATSUZAKI Yoko 山形大学, 医学部, 准教授 (00292417)
• Project Period (FY): 2009 – 2011
• Keywords: C型インフルエンザ / 迅速診断法

Research Abstract

Monoclonal antibodies were produced as the influenza C virus-specific antibodies for rapid diagnosis of influenza C virus infection and the real-time PCR assay for influenza C virus was newly developed. Respiratory specimens from children were examined for the presence of influenza C virus by cell culture and real-time PCR. Taking the cell culture results as the gold standard, the sensitivity, specificity, positive predictive value and negative predictive value for the real-time PCR were found to be 100%, 98.5%, 66.7% and 100%, respectively. The mean viral load in specimens that were positive by cell culture was significantly high than those in specimens that were negative by cell culture. The real-time PCR assay established here can be used as a sensitive method for diagnosing influenza C virus infection

Research Products

• [Journal Article] Detection and quantification of influenza C virus in pediatric respiratory specimens by real-time PCR and comparison with infectious viral counts (2012)
  • Author(s): Matsuzaki Y, Ikeda T, Abiko C, Aoki Y, Mizuta K, Shimotai Y, Sugawara K, Hongo S
  • Journal Title: J Clin Virol Volume: 54 Pages: 130-134
  • Peer Reviewed
• [Journal Article] Acute respiratory infections due to enterovirus 68 in Yamagata, Japan between 2005 and 2010 (2012)
  • Author(s): Ikeda T, Mizuta K, Abiko C, Aoki Y, Itagaki T, Katsushima F, Katsushima Y, Matsuzaki Y, Fuji N, Imamura T, Oshitani H, Noda M, Kimura H, Ahiko T
  • Journal Title: Microbiol Immunol Volume: 56(2) Pages: 139-143
  • Peer Reviewed
• [Journal Article] Saffold cardiovirus infection in children associated with respiratory disease and its similarity to coxsackievirus infection (2011)
  • Author(s): Itagaki T, Abiko C, Aoki Y, Ikeda T, Mizuta K, Noda M, Kimura H, Matsuzaki Y
  • Journal Title: Pediatr Infect Dis J Volume: 30(8) Pages: 680-683
  • Peer Reviewed
• [Journal Article] Palmitoylation of CM2 is dispensable to influenza C virus replication (2011)
  • Author(s): Muraki Y, Okuwa T, Furukawa T, Matsuzaki Y, Sugawara K, Himeda T, Hongo S, Ohara Y
  • Journal Title: Virus Res Volume: 157(1) Pages: 99-105
  • Peer Reviewed
• [Journal Article] ヒトメタニューモウイルス感染症の臨床経過とウイルス排出期間の検討 (2011)
  • Author(s): 板垣勉, 松嵜葉子
  • Journal Title: 日本小児科学会雑誌 Volume: 115(4) Pages: 782-787
  • Peer Reviewed
• [Journal Article] Comparison of virus isolation using the Vero E6 cell line with real-time RT-PCR assay for the detection of human metapneumovirus (2010)
  • Author(s): Matsuzaki Y, Mizuta K, Takashita E, Okamoto M, Itagaki T, Katsushima F, Katsushima Y, Nagai Y, Nishimura H
  • Journal Title: BMC Infect Dis Volume: 10 Pages: 170
  • DOI: DOI:10.1186/1471-2334-10-170
  • Peer Reviewed
• [Journal Article] Endemicity of human metapneumovirus subgenogroups A2 and B2 in Yamagata, Japan, between 2004 and 2009 (2010)
  • Author(s): Mizuta K, Abiko C, Aoki Y, Ikeda T, Itagaki T, Katsushima N, Matsuzaki Y, Hongo S, Noda M, Kimura H, Ahiko T
  • Journal Title: Microbiol Immunol Volume: 54(10) Pages: 634-638
  • Peer Reviewed
• [Journal Article] A two-year survey of the oseltamivir-resistant influenza A(H1N1) virus in Yamagata, Japan and the clinical effectiveness of oseltamivir and zanamivir (2010)
  • Author(s): Matsuzaki Y, Mizuta K, Aoki Y, Suto A, Abiko C, Sanjoh K, Sugawara K, Takashita E, Itagaki T, Katsushima Y, Ujike M, Obuchi M, Odagiri T, Tashiro M
  • Journal Title: Virol J Volume: 7(1) Pages: 53
  • DOI: DOI:10.1186/1743-422X-7-53
  • Peer Reviewed
• [Journal Article] C型インフルエンザの臨床的検討 (2009)
  • Author(s): 松嵜葉子
  • Journal Title: 小児科 Volume: 50(5) Pages: 637-642
• [Presentation] Antigenic structure of the hemagglutinin of pandemic influenza A(H1N1) virus (2011)
  • Author(s): Matsuzaki Y, Sugawara K, Shimotai Y, Hongo S, Nobusawa E
  • Organizer: International Union of Microbiological Societies 2011 Congress, Sapporo
  • Place of Presentation: Sapporo Convention Center
  • Year and Date: 2011-09-13
• [Presentation] リアルタイムPCR法を用いたC型インフルエンザウイルス検出の試み (2011)
  • Author(s): 松嵜葉子, 池田辰也, 青木洋子, 菅原勘悦, 下平義隆, 本郷誠治, 安孫子千恵子, 水田克巳
  • Organizer: 第65回日本細菌学会東北支部総会
  • Place of Presentation: 山形,山形大学医学部
  • Year and Date: 2011-08-18
• [Presentation] 地衛研と感染症臨床との連携 (2010)
  • Author(s): 松嵜葉子
  • Organizer: 第59回日本感染症学会東日本地方会学術集会
  • Place of Presentation: 東京,京王プラザホテル
  • Year and Date: 2010-10-21