Functional analysis of glucocorticoid induced transcript 1(GLCCI1)

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591398
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kyorin University
• Principal Investigator: YAN Kunimasa 杏林大学, 医学部, 教授 (70255389)
• Co-Investigator(Kenkyū-buntansha): AKIMOTO Yoshihiro 杏林大学, 医学部, 准教授 (60184115) ; ISHIGAKI Yasuhito 金沢医科大学, 医学部, 講師 (20232275)
• Project Period (FY): 2009 – 2011
• Keywords: 糖質ステロイド / Ｔ細胞 / アポトーシス

Research Abstract

The aim of the present study was to identify the protein function of GLCCI1, a novel gene induced by synthetic glucocorticoid. We found that GLCCI was a tubulin-associated phosphoprotein migrated at ～70 kDa that was induced via glucocorticoid-glucocorticoid receptor complex. We also found that both PAK1 anddynein-light chain 8 were the ligand proteins of GLCCI1, and that GLCCI1 interfered the PAK1 kinase activity against LC8 biogenesis, which led to T cell apoptosis. Our data suggested that the functional implication of GLCCI1 in PAK1-LC8 interaction might be a novel downstream cascade resembling synthetic glucocorticoid action as an immunosuppressant.