部位特異的な皮膚再生医療に関する研究

2009 Fiscal Year Annual Research Report

• Project/Area Number: 21591440
• Research Institution: Nagoya City University
• Principal Investigator: 山口 裕史 Nagoya City University, 大学院・医学研究科, 准教授 (00335340)
• Keywords: 線維芽細胞 / 表皮細胞 / メラノサイト / 再生医療 / 創傷治癒 / 皮膚癌 / 紫外線 / 悪性黒色腫

Research Abstract

マウス胎生期の皮膚形成には上皮系と間葉系との相互作用が重要であることが多数報告されている。我々も表皮のみにカテニンを強制発現させる系を用いて、相互作用の重要性を証明した。(Development 2009)また我々は「ヒト成人においても、上皮間葉系相互作用(真皮線維芽細胞がその上にある上反糸細胞に与える作用)により部位特異性(皮膚のさまざまな部位で性質が異なること)を決定付けていること」を実証してきた。(J Biol Chem 2007; Faseb J 2008)手のひら・足のうらの皮膚は、分厚い皮膚で外からの刺激に強い・毛が無い・色素が薄いという3つの特徴があり、手のひら・足のうらの真皮線維芽細胞で強発現しているDKK1の作用であることを報告してきた。今回は、ヒト胎生期においても上皮間葉系相互作用が重要であり、部位特異性があることを証明するために、中絶胎児より皮膚を4ヶ所(頭部、背部、腹部、足底)から生検し、相互作用を調べた。頭部ではメラノサイトの発現が他部位より早く成熟しており、毛包形成数は頭部、背部、腹部の順で多かった。足底には毛包構造が認められなかっだ。メラノサイトの分布も大きく違っていた。マウスにはない毛包間表皮のメラノサイトも発現を認めた。メラノサイトの数も頭部、背部、腹部、足底の順番に多く認めた。真皮内のメラノサイトは染色した抗体(7種類以上)で殆ど認めることがなかった。今後更に解析を進め、相互作用の部位特異性と皮膚形成メカニズムを解析していく予定である。

Research Products

• [Journal Article] NQO1 as an oxidoreductase enzyme that increases melanin synthesis (2010)
  • Author(s): Yamaguchi Y, Maeda A, Hearing VJ, Morita A.
  • Journal Title: J Invest Dermatol 130(3) Pages: 645-647
  • Peer Reviewed
• [Journal Article] Good's syndrome(hypogammaglobulinemia with thymoma)presenting intractable opportunistic infections and hyperkeratotic lichen planus (2010)
  • Author(s): Hanafusa T, Umegaki N, Yamaguchi Y, Katayama I.
  • Journal Title: J Dermatol 37(2) Pages: 171-174
  • Peer Reviewed
• [Journal Article] Glycoprotein nonmetastatic melanoma protein b(GPNMB), a melanocytic cell marker, is a melanosome-specific proteolytically released protein (2010)
  • Author(s): Hoashi T, Sato S, Yamaguchi Y, Passeron T, Tamaki K, Hearing VJ.
  • Journal Title: FASEB J (In Press, Epub ahead)
  • Peer Reviewed
• [Journal Article] Embryonic hair follicle fate change by augmented beta-catenin signaling through Shh and Bmp signaling (2009)
  • Author(s): Suzuki K, Yamaguchi Y, Villacorte M, Mihara K, Akiyama M, Shimizu H, Taketo MM, Nakagata N, Tsukiyama T, Yamaguchi TP, Kato S, Birchmeier W, Yamada G.
  • Journal Title: Development 136(3) Pages: 367-372
  • Peer Reviewed
• [Journal Article] The development of a filter to enhance the efficacy and safety of excimer light(308nm)therapy (2009)
  • Author(s): Kobayashi K, Yasuda Y, Shintani Y, Sumitomo T, Saga T, Kimura M, Yamamoto A, Mori T, Maeda A, Yamaguchi Y, Morita A.
  • Journal Title: Photodermatol Photoimmunol Photomed 25(1) Pages: 30-36
  • Peer Reviewed
• [Journal Article] Feasibility and accuracy of a newly developed hand-held device with a flat-type fluorescent lamp for measuring the minimal erythema dose for narrow-b and UVB therapy (2009)
  • Author(s): Morita A, Shintani Y, Nishida E, Kato H, Yoshida H, Minamoto M, Yamaguchi Y, Maeda A.
  • Journal Title: Photodermatol Photoimmunol Photomed 25(1) Pages: 41-44
  • Peer Reviewed
• [Journal Article] Stage III melanoma treated with chemotherapy after surgery during the second trimester of pregnancy (2009)
  • Author(s): Ishida I, Yamaguchi Y, Tanemura A, Hosokawa K, Itami S, Morita A, Katayama I.(Co-first and Corresponding author)
  • Journal Title: Arch Dermatol 145(3) Pages: 346-348
  • Peer Reviewed
• [Journal Article] Intractable wounds caused by pyoderma gangrenosum in a patient with critical limb ischemia treated with cyclosporine and adjuvant sympathectomy (2009)
  • Author(s): Takahashi Y, Yamaguchi Y, Itoh K, Sano S, Morita A, Katayama I.(Co-first and Corresponding author)
  • Journal Title: Dermatol Surg 35(4) Pages: 699-703
  • Peer Reviewed
• [Journal Article] Short- and long-term effects of ultraviolet radiation on the pigmentation of human skin (2009)
  • Author(s): Coelho SG, Choi W, Brenner M, Miyamura Y, Yamaguchi Y, Wolber R, Smuda C, Batzer J, Kolbe L, Ito S, Wakamatsu K, Zmudzka B, Beer JZ, Miller SA, Hearing VJ.
  • Journal Title: J Investig Dermatol Symp Proc 14(1) Pages: 32-35
  • Peer Reviewed
• [Journal Article] Molecular basis of premature skin aging induced by tobacco smoking (2009)
  • Author(s): Morita A, Torii K, Maeda A, Yamaguchi Y.
  • Journal Title: J Investig Dermatol Symp Proc 14(1) Pages: 53-55
  • Peer Reviewed
• [Journal Article] Regulation of skin pigmentation and thickness by dickkopf 1(DKK1) (2009)
  • Author(s): Yamaguchi Y, Morita A, Maeda A, Hearing VJ.
  • Journal Title: J Investig Dermatol Symp Proc 14(1) Pages: 73-75
  • Peer Reviewed
• [Journal Article] Epithelioid sarcoma on the foot masquerading as an intractable wound (2009)
  • Author(s): Nishimura Y, Yamaguchi Y, Hamada K, Tomita Y, Maeda A, Morita A, Katayama I.
  • Journal Title: Clin Exp Dermatol (IN PRESS, Epub)
  • Peer Reviewed
• [Journal Article] Physiological factors that regulate skin pigmentation (2009)
  • Author(s): Yamaguchi Y, Hearing VJ.
  • Journal Title: Biofactors 35(2) Pages: 193-199
  • Peer Reviewed
• [Journal Article] The effects of topically applied glycolic acid and salicylic acid on ultraviolet radiation-induced erythema, DNAdamage and sunburn cell formation in human skin (2009)
  • Author(s): Kornhauser A, Wei RR, Yamaguchi Y, Coelho SG, Kaidbey K, Barton C, Takahashi K, Beer JZ, Miller SA, Hearing VJ.
  • Journal Title: J Dermatol Sci 55(1) Pages: 10-17
  • Peer Reviewed
• [Presentation] The expression of dickkopf 1(DKK1)in palmoplantar skin is upregulated in vivo (2009)
  • Author(s): Yamaguchi Y, Hearing VJ, Suzuki K, Yamada G, Morita A.
  • Organizer: The 15^<th> Annual Meeting of the PanAmerican Society for Pigment Cell Research
  • Place of Presentation: Memphis TN, USA
  • Year and Date: 20090904-20090907
• [Presentation] Aiming for the ideal skin regenerative medicine via translational research (2009)
  • Author(s): Yamaguchi Y.
  • Organizer: JST-Swiss Federal Institute of Technology Zurich Seminar
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-11-05
• [Presentation] Aiming for the ideal skin regenerative medicine via translational research : adult dermal fibroblasts determine the fate of the overlying epidermis (2009)
  • Author(s): Yamaguchi Y.
  • Organizer: Laboratory of Cell Biology Seminar at National Cancer Institute, National Institutes of Health
  • Place of Presentation: Bethesda, MD, USA
  • Year and Date: 2009-09-01
• [Book] 看護学テキストNiCE 疾患論 第17章 皮膚疾患 色素異常症 (2010)
  • Author(s): 山口裕史
  • Total Pages: 500
  • Publisher: 南光堂(In press)
• [Book] 新皮膚科レジデント戦略ガイド (2010)
  • Author(s): 山口裕史
  • Publisher: 診断と治療社(In press)
• [Book] からだと光の事典 (2010)
  • Author(s): 山口裕史
  • Publisher: 朝倉書店(In press)
• [Remarks]
  • URL: http://read.jst.go.jp/