2011 Fiscal Year Final Research Report
New melanoma model using transgenic medaka fish
| Project/Area Number |
21591444
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SAYA Hideyuki 慶應義塾大学, 医学部, 教授 (80264282)
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| Project Period (FY) |
2009 – 2011
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| Keywords | メダカ / 遺伝子導入 / 癌モデル生物 |
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| Research Abstract |
The purpose of this study is to construct the stable tumor bearing medaka line and to apply the line to drug screening. We intended to establish the melanoma model which can be detected tumors without autopsy for chemical screening in water tank. With cross transgenic(Tg)-tyr : HRAS^<G12V> medaka with Tg-hsp : cre medaka, we could obtain medaka line induced human HRAS^<G12V> by heat treatment. All of the double Tg medaka had melanophore hyperplasia or melanoma at six month post fertilization. Preliminary administration of sorafenib to this double Tg medaka having tumor showed significant inhibition of tumor growth and elongation of longevity.
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Research Products
(15 results)
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[Journal Article] Induction of ZEB by inactivation of RB is a key determinant of the mesenchymal phenotype of breast cancer2012
Author(s)
Arima Y, Hayashi H, Sasaki M, Hosonaga M, Goto TM, Chiyoda T, Kuninaka S, Shibata T, Ohata H, Nakagama H, Taya Y and Saya H
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Journal Title
J Biol Chem
Volume: 287
Pages: 7896-7906
Peer Reviewed
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[Journal Article] Modulation of glucose metabolism by CD44 contributes to antioxidant status and drug resistance in cancer cells2012
Author(s)
Tamada M, Nagano O, Tateyama S, Ohmura M, Yae T, Ishimoto T, Sugihara E, Onishi N, Yamamoto T, Yanagawa H, Suematsu M and Saya H
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Journal Title
Cancer Res
Volume: 72
Pages: 1438-1448
Peer Reviewed
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[Journal Article] Invasion precedes tumor mass formation in a malignant brain tumor model of genetically modified neural stem cells2011
Author(s)
Sampetrean O, Saga I, Nakanishi M, Sugihara E, Fukaya R, Onishi N, Osuka A, Akahata M, Kai K, Sugimoto H, Hirao A, and Saya H
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Journal Title
Neoplasia
Volume: 13
Pages: 784-791
Peer Reviewed
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