2011 Fiscal Year Final Research Report
New melanoma model using transgenic medaka fish
| Project/Area Number |
21591444
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SAYA Hideyuki 慶應義塾大学, 医学部, 教授 (80264282)
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| Project Period (FY) |
2009 – 2011
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| Keywords | メダカ / 遺伝子導入 / 癌モデル生物 |
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| Research Abstract |
The purpose of this study is to construct the stable tumor bearing medaka line and to apply the line to drug screening. We intended to establish the melanoma model which can be detected tumors without autopsy for chemical screening in water tank. With cross transgenic(Tg)-tyr : HRAS^<G12V> medaka with Tg-hsp : cre medaka, we could obtain medaka line induced human HRAS^<G12V> by heat treatment. All of the double Tg medaka had melanophore hyperplasia or melanoma at six month post fertilization. Preliminary administration of sorafenib to this double Tg medaka having tumor showed significant inhibition of tumor growth and elongation of longevity.
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