血小板を介する皮膚の炎症反応の制御・収束機構の解明

2009 Fiscal Year Annual Research Report

• Project/Area Number: 21591472
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: 加藤 則人 Kyoto Prefectural University of Medicine, 医学研究科, 教授 (30244578)
• Co-Investigator(Kenkyū-buntansha): 浅井 純 京都府立医科大学, 医学研究科, 助教 (50438222)
• Keywords: アトピー性皮膚炎 / 血小板 / CD11b+Gr-1+細胞 / 可視化

Research Abstract

マウス接触過敏反応の皮膚を、抗CD41抗体と抗CD3抗体、抗CD31抗体で三重染色することにより、皮膚組織における白血球・血小板複合体と血管との関係を可視化することに成功した。また、抗CD3抗体の代わりに抗CD11b抗体や抗GR-1抗体を用いることにより、ミエロイドサプレッサー細胞と血小板の関係についても可視化することに成功した。さらに血小板減少マウスにGFPトランスジェニックマウス由来の血小板を静脈注射した後に皮膚炎を惹起することにより、血小板と血管、白血球の動態をさらに詳細に可視化することができた。いずれの実験も、静止画に加えて動画による記録を行うことができた。
皮膚炎回復後の真皮樹状細胞の皮膚組織への再分布についても、血小板、特に血小板が表面に発現するP-セレクチンが極めて重要な働きをしていることが明らかになった。
今後は、皮膚組織で血小板が遊離するケモカインなどの物質を可視化することにより、血小板を介する皮膚炎の制御機構の解析をさらに詳細に行っていきたい。

Research Products

• [Journal Article] Platelet activation in patients with psoriasis : increased plasma levels of platelet-derived microparticles and soluble P-selectin. (2010)
  • Author(s): Tamagawa-Mineoka R, Katoh N, Kishimoto S.
  • Journal Title: J Am Acad Dermatol 62 Pages: 621-626
  • Peer Reviewed
• [Journal Article] IL-12 gene transfer cooperates with Mitf silencing to inhibit melanoma in mice. (2010)
  • Author(s): Nakai N, Kishida T, Hartmann G, Katoh N, Imanishi J, Kishimoto S, Mazda O.
  • Journal Title: Int Immunopharmacol 10 Pages: 540-545
  • Peer Reviewed
• [Journal Article] Anaphylaxis to abalone that was diagnosed by prick test of abalone extracts and immunoblotting for serum immunoglobulin E. (2010)
  • Author(s): Masuda K, Tashima S, Katoh N.
  • Journal Title: Int J Dermatol (印刷中)
  • Peer Reviewed
• [Journal Article] Persistent congenital milia involving the skin of the whole body in an infant with trisomy 13 syndrome. (2010)
  • Author(s): Nakai N, Okuzawa Y, Katoh N, Kishimoto S.
  • Journal Title: Pediatr Dermatol (印刷中)
  • Peer Reviewed
• [Journal Article] Basal cell carcinoma with a skip lesion on the nose after repeated cryotherapy. (2010)
  • Author(s): Nakai N, Takenaka H, Katoh N, Kishimoto S.
  • Journal Title: J Dermatol 37 Pages: 390-392
  • Peer Reviewed
• [Journal Article] Systemic allergic contact dermatitis due to phenylephrine in eyedrops, with a Iong-lasting allergic patch test reaction. (2010)
  • Author(s): Tamagawa-Mineoka R, Katoh N, Yoneda K, Cho Y, Kishimoto S.
  • Journal Title: Eur J Dermatol 20 Pages: 125-126
  • Peer Reviewed
• [Journal Article] Platelets as versatile regulators of cutaneous inflammation. (2009)
  • Author(s): Katoh N.
  • Journal Title: J Dermatol Sci 53 Pages: 89-95
  • Peer Reviewed
• [Journal Article] Immunohistological analysis of peptide-induced delayed-type hypersensitivity inadvanced melanoma patients treated with melanoma antigen-pulsed mature monocyte-derived dendritic cell vaccination. (2009)
  • Author(s): Nakai A, Katoh N, Germeraad WT, Kishida T, Ueda E, Takenaka H, Mazda O, Kishimoto S.
  • Journal Title: J Dermatol Sci 53 Pages: 40-47
  • Peer Reviewed
• [Journal Article] Symmetrical drug-related intertrigunous and flexural exanthema (SDRIFE) eruptioncaused by valacyclovir. (2009)
  • Author(s): Daito J, Hanada K, Katoh N, Katoh S, Sakamoto K, Asai J, Takenaka H, Kishimoto S.
  • Journal Title: Dermatology 218 Pages: 60-62
  • Peer Reviewed
• [Journal Article] Atopic dermatitis, dry skin and serum IgE in children in a community in Japan. (2009)
  • Author(s): Wakamori T, Katoh N, Hirano S, Kishimoto S, Ozasa K.
  • Journal Title: Int Arch Allergy Immunol 149 Pages: 103-110
  • Peer Reviewed
• [Journal Article] Future perspectives in the treatment of atopic dermatitis. (2009)
  • Author(s): Katoh N.
  • Journal Title: J Dermatol 36 Pages: 367-376
  • Peer Reviewed
• [Journal Article] Granulomatous mycosis fungoides presenting as poikiloderma. (2009)
  • Author(s): Morihara K, Katoh N, Takenaka H, Morihara T, Kishimoto S.
  • Journal Title: Clin Exp Dermatol 34 Pages: 718-720
  • Peer Reviewed
• [Journal Article] Hyperpigmentation on the thumb with a clinical resemblance to malignant melanoma caused by chronic irritation due to sewing. (2009)
  • Author(s): Nakai N, Katoh N, Kishimoto S.
  • Journal Title: Clin Exp Dermatol 34 Pages: e423-424
  • Peer Reviewed
• [Journal Article] Dichotomous effect of ultraviolet B on the expression of corneodesmosomal enzymes in human keratinocytes. (2009)
  • Author(s): Nin M, Katoh N, Kokura S, Handa O, Yoshikawa T, Kishimoto S.
  • Journal Title: J Dermatol Sci 54 Pages: 17-24
  • Peer Reviewed
• [Journal Article] Anaphylaxis to vitamin B2 added to an energy drink. (2009)
  • Author(s): Masuda K, Katoh N, Mizutani H, Kishimoto S.
  • Journal Title: Clin Exp Dermatol 34 Pages: e263-264
  • Peer Reviewed
• [Journal Article] Keratinocyte growth inhibition through the modification of Wnt signaling by androgenin balding dermal papilla cells. (2009)
  • Author(s): Kitagawa T, Matsuda KI, Inui S, Takenaka H, Katoh N, Itami S, Kishimoto S, Kawata M.
  • Journal Title: J Clin Endcrinol Metab 94 Pages: 1288-1294
  • Peer Reviewed
• [Journal Article] Platelets play important roles in the late phase of the immediate hypersensitivity reaction. (2009)
  • Author(s): Tamagawa-Mineoka R, Katoh N, Kishimoto S.
  • Journal Title: J Allergy Clin Immunol 94 Pages: 1288-1294
  • Peer Reviewed
• [Journal Article] Allergic contact dermatitis due to tribenoside (2009)
  • Author(s): Inoue A, Tamagawa-Mineoka R, Katoh N, Kishimoto S.
  • Journal Title: Contact Dermatitis 60 Pages: 349-350
  • Peer Reviewed
• [Journal Article] Platelet-derived microparticles and soluble P-selectin as platelet activation markers in patients with atopic dermatitis. (2009)
  • Author(s): Tamagawa-Mineoka R, Katoh N, Ueda E, Masuda K, Kishimoto S.
  • Journal Title: Clin Immunol 131 Pages: 495-500
  • Peer Reviewed
• [Journal Article] The role of IL-33 in chronic allergic conjunctivitis. (2009)
  • Author(s): Matsuda A, Okayama Y, Terai N, Yokoi N, Ebihara N, Tanioka H, Kawasaki S, Inatomi T, Katoh N, Ueda E, Hamuro J, Murakami A, Kinoshita S.
  • Journal Title: Invest Ophthalmol Vis Sci 50 Pages: 4646-4652
  • Peer Reviewed
• [Journal Article] Eosinophilic folliculitis with a Demodex folliculorum infestation in HIV-infected individual successfully treated with ivermectin. (2009)
  • Author(s): Nara T, Katoh N, Inoue K, Yamada M, Arizono N, Kishimoto S.
  • Journal Title: Clin Exp Dermatol 34 Pages: e981-983
  • Peer Reviewed
• [Journal Article] A case of autoimmune progesterone dermatitis presenting as fixed drug eruption. (2009)
  • Author(s): Asai J, Katoh N, Nakano M, Wada M, Kishimoto S.
  • Journal Title: J Dermatol 36 Pages: 643-645
  • Peer Reviewed
• [Presentation] What's new in immunology of atopic dermatitis. (2009)
  • Author(s): Katoh N.
  • Organizer: 17th International Contact Dermatitis Symposium & 10th Asia-Pacific Environmental and Occupational Dermatology Symposium.
  • Place of Presentation: Kyoto, Japan.
  • Year and Date: 2009-11-06
• [Remarks]
  • URL: http://kpum-dermatology.jp/