2011 Fiscal Year Final Research Report
Identification of biomarkers for differential diagnosis of neurodegenerative and non-neurodegenerative dementia
| Project/Area Number |
21591489
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
SOHMA Hitoshi 札幌医科大学, 医療人育成センター, 教授 (70226702)
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| Co-Investigator(Kenkyū-buntansha) |
KOKAI Yasuo 札幌医科大学, 医学部, 教授 (20178239)
SAITO Toshikazu 札幌医科大学, 医学部, 教授 (50128518)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 認知症 / アルツハイマー病 / プロテオーム解析 / 血漿バイオマーカー / アネキシンA5 / MFG-E8 |
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| Research Abstract |
Alzheimer's disease(AD) differs from other forms of dementia in its relation to amyloid beta peptide(Aβ_<42>), which has a toxic effect on neuronal cells. This effect implies that protein expression is changed in neuronal cells by Aβ42. In the present study, we used the mice primary culture neurons and investigated the proteins in the supernatant after incubation with or without Aβ_<42>. In view of the appearance of an acidic phospholipid(phosphatidylserine(PS)) on the outer plasma membrane of an apoptotic cell, we used PS as a probe and proteins bound to PS-coated magnetic nano-beads were identified using a proteomic approach. Of a number of proteins identified, we focused on annexin A5 and milk fat globule-EGF-factor 8(MFG-E8) that is involved in the clearance of apoptotic cells. Both annexin A5 and MFG-E8 were found to be increased significantly in the culture supernatant by Aβ_<42>.
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[Journal Article] Pulmonary Surfactant Protein A Protects Lung Epithelium from Cytotoxicity of Humanβ-Defensin 32012
Author(s)
Saito A, Ariki S, Sohma H, Nishitani C, Inoue K, Ebata N, Takahashi M, Hasegawa Y, Kuronuma K, Takahashi H, Kuroki Y
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Journal Title
J Biol Chem
Volume: 287
Pages: 15034-15043
Peer Reviewed
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[Journal Article] Metallothionein deficiency in the injured peripheral nerves of complex regional pain syndrome as revealed by proteomics2012
Author(s)
Oki G, Wada T, Iba K, Aiki H, Sasaki K, Imai S, Sohma H, Matsumoto K, Yamaguchi M, Fujimiya M, Yamashita T, Kokai Y
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Journal Title
Pain
Volume: 153
Pages: 532-539
Peer Reviewed
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[Journal Article] Early expression of plasma CCL8 closely correlates with survival rate of acute graft-versus-host disease in mice2011
Author(s)
Yamamoto M, Ota A, Hori T, Imai S, Sohma H, Suzuki N, Hatakeyama N, Inazawa N, Ito YM, Kimura H, Tsutsumi H, Kokai Y
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Journal Title
Exp. Hematol
Volume: 39
Pages: 1101-1112
Peer Reviewed
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[Journal Article] Investigation of annexin A5 as a biomarker for Alzheimer's disease using neuronal cell culture and mouse model2010
Author(s)
Yamaguchi M, Kokai Y, Imai S, Utsumi K, Matsumoto K, Honda H, Mizue Y, Momma M, Maeda T, Toyomasu S, Ito YM, Kobayashi S, Hashimoto E, Saito S, Sohma H
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Journal Title
J. Neurosci. Res
Volume: 88
Pages: 2682-2692
Peer Reviewed
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