Chemoradiotherapy targeting autophagy mechanism against pancreas cancer.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591609
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: Kobe University
• Principal Investigator: OKAMOTO Yoshiaki 神戸大学, 大学院・医学研究科, 非常勤講師 (20362791)
• Co-Investigator(Kenkyū-buntansha): SASAKI Ryohei 神戸大学, 医学部附属病院, 特命准教授 (30346267) ; SUGIMURA Kazuro 神戸大学, 大学院・医学研究科, 教授 (00136384) ; NISHIMURA Hideki 神戸大学, 医学部附属病院, 特命講師 (80444610) ; YOSHIDA Kenji 神戸大学, 医学部附属病院, 助教 (80351906) ; SUZUKI Yoko 神戸大学, 大学院・医学研究科, 技術補佐員 (70448182) ; TSUJIMURA Toshiaki 神戸大学, 大学院・医学研究科, 医学研究員 (30403249)
• Project Period (FY): 2009 – 2011
• Keywords: オートファジー / 放射線療法 / 膵癌

Research Abstract

Autophagy is one of the most important intraceller mechanism to radiation and chemotherapy. In this study, we evaluated induction of the autophagy by using human pancreas cancer cells. Autophagy was assessed by electron microscope, western blotting of LC-3. In vivo experiment, gemcitabine and ionizing radiation resulted in synergistic cytotoxicity with induction of autophagy.

Research Products

• [Journal Article] Multi-institutional analysis of solitary extramedullary plasmacytoma of the head and neck treated with curative radiotherapy (2012)
  • Author(s): Sasaki R, Yasuda K, Abe E, Uchida N, Kawashima M, Uno T, Fujiwara M, Shioyama Y, Kagami Y, Shibamoto Y, Nakata K, Takada Y, Kawabe T, Uehara K, Nibu K, Yamada S
  • Journal Title: Int J Radiat Oncol Biol Phys Volume: 82(2) Pages: 626-34
• [Journal Article] Long-term outcome and patterns of failure in primary ocular adnexal mucosa-associated lymphoid tissue lymphoma treated with radiotherapy (2012)
  • Author(s): Hashimoto N, Sasaki R, Nishimura H, Yoshida K, Miyawaki D, Nakayama M, Uehara K, Okamoto Y, Ejima Y, Azumi A, Matsui T, Sugimura K
  • Journal Title: Int J Radiat Oncol Biol Phys Volume: 82(4) Pages: 1509-14
• [Journal Article] Radiotherapy for Japanese elderly patients with cervical cancer : preliminary survival outcomes and evaluation oftreatment-related toxicity (2011)
  • Author(s): Yoshida K, Sasaki R, Nishimura H, Miyawaki D, Kawabe T, Okamoto Y, Nakabayashi K, Yoshida S, Sugimura K
  • Journal Title: Arch Gynecol Obstet Volume: 284(4) Pages: 1007-14
• [Journal Article] The effectiveness of particle radiotherapy for hepatocellular carcinoma associated with inferior vena cava tumor thrombus (2011)
  • Author(s): Komatsu S, Fukumoto T, Demizu Y, Miyawaki D, Terashima K, Niwa Y, Mima M, Fujii O, Sasaki R, Yamada I, Hori Y, Hishikawa Y, Abe M, Ku Y, Murakami M
  • Journal Title: J Gastroenterol Volume: 46(7) Pages: 913-20
• [Journal Article] 耳下腺癌における予防的頸部郭清と術後放射線治療について (2011)
  • Author(s): 柴田智久, 米澤宏一郎, 森本浩一, 山下大介, 大月直樹, 西村英輝, 佐々木良平, 丹生健一
  • Journal Title: 頭頸部癌 Volume: 37巻1号 Pages: 78-82
• [Journal Article] 局所進行下咽頭癌に対する術後放射線療法の遡及的検討 (2011)
  • Author(s): 西村英輝, 佐々木良平, 吉田賢史, 宮脇大輔, 大月直樹, 斉藤幹, 清田尚臣, 副島俊典, 杉村和朗, 丹生健一
  • Journal Title: 頭頸部癌 Volume: 37巻1号 Pages: 62-66
• [Journal Article] Nuclear factor-kappaB expression as a novel marker of radioresistance in early-stage laryngeal cancer (2010)
  • Author(s): Yoshida K, Sasaki R, Nishimura H, Okamoto Y, Suzuki Y, Kawabe T, Saito M, Otsuki N, Hayashi Y, Soejima T, Nibu K, Sugimura K
  • Journal Title: Head Neck Volume: 32(5) Pages: 646-55
• [Journal Article] Enhancement of human cancer cell radiosensitivity by conjugated eicosapentaenoic acid-a mammalian DNA polymerase inhibitor (2010)
  • Author(s): Kumamoto-Yonezawa Y, Sasaki R, Suzuki Y, Matsui Y, Hada T, Uryu K, Sugimura K, Yoshida H, Mizushina Y
  • Journal Title: Int J Oncol Volume: 36(3) Pages: 577-84
• [Journal Article] The role of autophagy in the treatment of pancreatic cancer with gemcitabine and ionizing radiation (2010)
  • Author(s): Mukubou H, Tsujimura T, Sasaki R, Ku Y
  • Journal Title: Int J Oncol Volume: 37(4) Pages: 821-8
• [Journal Article] Vitamin K2-derived compounds induce growth inhibition in radioresistant cancer cells (2010)
  • Author(s): Amalia H, Sasaki R, Suzuki Y, Demizu Y, Bito T, Nishimura H, Okamoto Y, Yoshida K, Miyawaki D, Kawabe T, Mizushina Y, Sugimura K
  • Journal Title: Kobe J Med Sci Volume: 56(2) Pages: E38-49
• [Presentation] Nuclear factor-kappa B Expression as A Novel Marker of Radioresistance in Early-stage Laryngeal Cancer (2011)
  • Author(s): R Sasaki, K Yoshida, H Nishimura, D Miyawaki, M Saito, N Otsuki, K Nibu
  • Organizer: 11th Japan-Taiwan Conference on Otolaryngology-Head and Neck Surgery
  • Place of Presentation: Kobe
  • Year and Date: 20111207-08
• [Presentation] Dosimetric Analysis of Patterns of Failure for High-grade Glioma with Multimodality Treatment : Relationship to MIB-1Labeling Index (2011)
  • Author(s): K Uehara, R Sasaki, D Miyawaki, H Nishimura, K Yoshida, M Nakayama, T Soejima, O Fujii, T Sasayama, K Sugimura
  • Organizer: American Society of Therapeutic Radiation Oncology(ASTRO) 53rd Annual Meeting
  • Place of Presentation: Miami FL, USA
  • Year and Date: 20111002-06
• [Presentation] Long-term Outcome and Patterns of Failure in Primary Ocular Adnexal MALT Lymphoma Treated with Radiotherapy (2011)
  • Author(s): N Hashimoto, R Sasaki, H Nishimura, K Yoshida, D Miyawaki, Y Okamoto, Y Ejima, A Azumi, T Matsui, K Sugimura
  • Organizer: American Society of Therapeutic Radiation Oncology(ASTRO) 53rd Annual Meeting
  • Place of Presentation: Miami FL, USA
  • Year and Date: 20111002-06
• [Presentation] Microplanar Beams Radiation : Is Brain Really A Serial Organ? (2011)
  • Author(s): N Mukumoto, R Sasaki, H Akasaka, M Nakayama, D Miyawaki, H Nishimura, K Umetani, T Kondoh, K Shinohara, K Sugimura
  • Organizer: American Society of Therapeutic Radiation Oncology(ASTRO) 53rd Annual Meeting
  • Place of Presentation: Miami FL, USA
  • Year and Date: 20111002-06
• [Presentation] Radiation Therapy For Brain Stem Gliomas In Children And Adults (2011)
  • Author(s): K Yoshida, H Nishimura, D Miyawaki, O Muraoka, K Uehara, M Nakayama, R Sasaki, K Sugimura
  • Organizer: American Society of Therapeutic Radiation Oncology(ASTRO) 53rd Annual Meeting
  • Place of Presentation: Miami FL, USA
  • Year and Date: 20111002-06
• [Presentation] Combined Modality Therapy Including Radiotherapy for Squamous Cell Carcinomas of Maxillary Sinus-a Retrospective Study (2011)
  • Author(s): D Miyawaki, H Nishimura, K Yoshida, O Muraoka, M Nakayama, K Uehara, K Sugimura, S Hasegawa, K Nibu, R Sasaki
  • Organizer: European Cancer Conference(ECCO) 16th Annual Meeting
  • Place of Presentation: Stokholm
  • Year and Date: 20110924-28
• [Presentation] Radiotherapy for Elderly Patients with Cervical Cancer (2011)
  • Author(s): K Yoshida, H Nishimura, D Miyawaki, O Muraoka, A Harada, N S Sulaiman, K Nakabayashi, S Yoshida, R Sasaki, K Sugimura
  • Organizer: European Cancer Conference(ECCO) 16th Annual Meeting
  • Place of Presentation: Stokholm
  • Year and Date: 20110924-28
• [Presentation] Radiotherapy for Elderly Patients with Cervical Cancer (2010)
  • Author(s): K. Yoshida, H. Nishimura, D. Miyawaki, T. Kawabe, Y. Okamoto, K. Nakabayashi, S. Yoshida, R. Sasaki, K. Sugimura
  • Organizer: American Society of Therapeutic Radiation Oncology(ASTRO) 51st Annual Meeting
  • Place of Presentation: San Diego, CA USA
  • Year and Date: 20101031-1104
• [Presentation] Significance of Autophagy Induced by Gemcitabine, Radiotherapy, and the Combination of Them against Pancreatic Adenocarcinoma In Vitro and In Vivo (2009)
  • Author(s): H Mukubou, T Tsujimura, R Sasaki, K Yoshida, Y Suzuki, Y Hori, Y Ku
  • Organizer: American Society of Therapeutic Radiation Oncology(ASTRO) 51st Annual Meeting
  • Place of Presentation: Chicago, IL USA
  • Year and Date: 20091031-1105
• [Presentation] Radiotherapy for Locally Advanced Hypopharyngeal Carcinoma-A Comparison between Definitive (2009)
  • Author(s): H. Nishimura, R. Sasaki, Y. Okamoto, K. Yoshida, Kawabe T, K. Nibu, N. Ohtsuki, M. Saitoh
  • Organizer: American Society of Therapeutic Radiation Oncology(ASTRO) 51st Annual Meeting
  • Place of Presentation: Chicago, IL USA
  • Year and Date: 20091031-1105
• [Presentation] Treatment Outcomes of Squamous Cell Carcinoma of External Auditory Canal and Middle Ear (2009)
  • Author(s): K. Yoshida, R. Sasaki, M. Nakabayashi, H. Nishimura, Y. Okamoto, Kawabe T, S. Hasegawa, N. Otsuki
  • Organizer: American Society of Therapeutic Radiation Oncology(ASTRO) 51st Annual Meeting
  • Place of Presentation: Chicago, IL USA
  • Year and Date: 20091031-1105