Evaluation of improvement of vascularization for transplanted islet with PBSCs

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591654
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Clinical research Center, Chiba-East National Hospital, National Hospital Organization
• Principal Investigator: ITO Taihei 独立行政法人国立病院機構(千葉東病院臨床研究センター) (60509701)
• Co-Investigator(Kenkyū-buntansha): KENMOCHI Takashi 独立行政法人国立病院機構(千葉東病院臨床研究センター), 臨床研究センター, センタ長 (50215133) ; MARUYAMA Michihiro 独立行政法人国立病院機構(千葉東病院臨床研究センター), 分子生物研究室, 室長 (40399754) ; AKUTSU Naotake 独立行政法人国立病院機構(千葉東病院臨床研究センター), 移植再生医療研究部, 部長 (00344979) ; OTSUKI Kazunori 独立行政法人国立病院機構(千葉東病院臨床研究センター), 実験治療開発研究室, 室長 (50399755)
• Project Period (FY): 2009 – 2011
• Keywords: 膵島移植 / 血管再生医療 / 末梢血幹細胞 / 糖尿病

Research Abstract

In this study we established culture of both PBSCs and MSCs from canine. Now, we are trying to establish canine islet transplantation modelwith marginal number and compare between those control model and PBSCs or MSCsco-transplant model in terms of glucose tolerance.

Research Products

• [Journal Article] Mesenchymal stemcells facilitate mixed hematopoieticchimerism induction and prevent onset ofdiabetes in nonobese diabetic mice (2011)
  • Author(s): Asari S, Itakura S, Rawson J, Ito T, Todorov I, Nair I, Shintaku J, Liu CP, Kandeel F, Mullen YS
  • Journal Title: Pancreas Volume: 40(6) Pages: 846-54
  • Peer Reviewed
• [Journal Article] Mesenchymal stemcell and islet co-transplantationpromotes graft revascularization andfunction (2010)
  • Author(s): Ito T, Itakura S, Todorov I, Rawson J, Asari S, Shintaku J, Nair I, Ferreri K, Kandeel F, Mullen Y.
  • Journal Title: Transplantation Volume: 89(12) Pages: 1438-45
  • Peer Reviewed
• [Journal Article] Quantitative assessment ofβ-cell apoptosis and cell compositionof isolated, undisrupted human islets bylaser scanning cytometry (2010)
  • Author(s): Todorov I, Nair I, Avakian-MansoorianA, Rawson J, Omori K, Ito T, Valiente L, Iglesias-Meza I, Orr C, Shiang KD, Ferreri K, Al-Abdullah IH, Mullen Y, Kandeel F.
  • Journal Title: Transplantation Volume: 90(8) Pages: 836-42
  • Peer Reviewed
• [Presentation] 膵島分離における膵臓保存時の膵管内注入保存液の検討 (2011)
  • Author(s): 伊藤泰平、剣持敬、西郷健一、丸山通広、圷尚武、大月和宣、岩下力、齋藤友永、浅野武秀
  • Organizer: 第38回日本膵膵島移植学会
  • Place of Presentation: 奈良
  • Year and Date: 2011-03-04
• [Presentation] イヌ膵島移植モデルへの応用に向けたイヌ間葉系幹細胞(cMSC)の分離、培養 (2010)
  • Author(s): 斉藤友永、剣持敬、伊藤泰平、西郷健一、丸山通広、圷尚武、岩下力、大月和宣、浅野武秀
  • Organizer: 第37回日本臓器保存生物医学会
  • Place of Presentation: 新潟
  • Year and Date: 20101119-20
• [Presentation] Canine Mesenchymal Stem Cell(cMSCs)の分離・培養-大動物膵島移植実験への応用にむけて- (2010)
  • Author(s): 斉藤友永、剣持敬、伊藤泰平、西郷健一、丸山通広、圷尚武、岩下力、大月和宣、浅野武秀
  • Organizer: 第46回日本移植学会
  • Place of Presentation: 京都
  • Year and Date: 20101020-22
• [Presentation] 膵島移植におけるMesenchymalstem cells(MSCs)を用いたGenedelivery system (2010)
  • Author(s): 伊藤泰平、剣持敬、西郷健一、丸山通広、圷尚武、大月和宣、岩下力、齋藤友永、浅野武秀
  • Organizer: 第46回日本移植学会
  • Place of Presentation: 京都
  • Year and Date: 20101020-22
• [Presentation] WST-1/DNA assayによる膵島viability測定 (2010)
  • Author(s): 伊藤泰平,剣持敬,西郷健一,丸山通広,圷尚武,大月和宣,岩下力,齊藤友永
  • Organizer: 第37回膵膵島移植学会
  • Place of Presentation: 宇都宮
  • Year and Date: 20100312-14
• [Presentation] A new viability assay of isletusing WST-1/DNA (2010)
  • Author(s): Taihei Ito, Takashi Kenmochi, Michihiro Maruyama, Kenichi Saigo, Naotake Akutsu, Chikara Iwashita, Kazunori Otsuki, Saito Tomonori, Takehide Asano
  • Organizer: XXIII International Congress of The Transplantation
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2010-08-16
• [Presentation] The application of luciferase transgenicrats for experimental islettransplantation model (2009)
  • Author(s): Taihei Ito、Takashi Kenmochi、KennichiSaigo、Michihiro Maruyama、NaotakeAkutsu、Kazunori Ohtsuki、ChikaraIwashita、Eiji Kobayashi、Takehide Asano
  • Organizer: IPITA-IXA2009
  • Place of Presentation: Venis, Italy
  • Year and Date: 20091012-16
• [Presentation] UW solution improvesislet yield as a solution forpancreatic duct preservationbetter than ET-Kyoto solution
  • Author(s): Taihei Ito、TakashiKenmochi、Michihiro Maruyama、Naotake Akutsu、KazunoriOhtsuki、Chikara Iwashita、Matsumoto Ikuko、Takehide Asano
  • Organizer: 12th Congress of the Asian