Estrogen receptor knock down therapy using microRNA for breast cancer patients

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591674
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Nagoya City University
• Principal Investigator: TOYAMA Tatsuya 名古屋市立大学, 大学院・医学研究科, 准教授 (30315882)
• Co-Investigator(Kenkyū-buntansha): YAMASHITA Hiroko 名古屋市立大学, 大学院・医学研究科, 准教授 (70332947) ; SUGIURA Hiroshi 名古屋市立大学, 大学院・医学研究科, 研究員 (20381882)
• Project Period (FY): 2009 – 2011
• Keywords: 乳癌 / マイクロRNA / エストロゲン・レセプター

Research Abstract

Several microRNAs(miRNAs) that directly target ERα have been identified. In this study, expression levels of miRNAs that directly target ERα, including miR-18a, miR-18b, miR-22, miR-193b, miR-206, miR-221/222 and miR-302c, were analyzed in human breast cancer samples by quantitative reverse transcription-PCR analysis. Correlations between the expression levels of these miRNAs and clinicopathological factors were analyzed. miR-18a expression was much higher in ERα-negative than in ERα-positive tumors, with the expression levels of miR-18a not differing in ERα-positive breast cancer as a function of ERαprotein level. Surprisingly, the expression levels of miR-193b and miR-221 were significantly lower in ERα-negative than in ERα-positive tumors, and the levels of these miRNAs gradually increased as ERα protein expression increased. Our results suggest that miR-206 could be a novel candidate for endocrine therapy that targets only ERα in breast cancer.

Research Products

• [Journal Article] Distinct expressions of microRNAs that directly target estrogen receptor α in human breast cancer (2011)
  • Author(s): Yoshimoto N, Toyama T, Takahashi S, Sugiura H, Endo Y, Iwasa M, Fujii Y, Yamashita H
  • Journal Title: Breast Cancer Res Treat Volume: 130(1) Pages: 331-9
• [Presentation] Low Expression of microRNA-210 Is an Independent Good Prognostic Factor in Japanese Triple-Negative Breast Cancer Patients (2011)
  • Author(s): Toyama T, Kondo N, Endo Y, Sugiura H, Yoshimoto N, Iwasa M, Takahashi S, Iwase H, Fujii Y, Yamashita H
  • Organizer: 34rd Annual San Antonio Breast Cancer Symposium
  • Place of Presentation: San Antonio, USA
  • Year and Date: 20111206-10
• [Presentation] Distinct expressions of microRNAs that directly target estrogen receptor in human breast cancer (2011)
  • Author(s): Yamashita H, Yoshimoto N, Toyama T, Takahashi S, Sugiura H, Endo Y, Iwasa M, Fujii Y
  • Organizer: Advances in Breast Cancer Research, American Association for Cancer Research(AACR) Special Conference in Cancer Research
  • Place of Presentation: San Francisco, U. S. A.
  • Year and Date: 20111012-15
• [Presentation] 乳癌のエストロゲンレセプターαの発現調節に関与するmicroRNAの解析 (2011)
  • Author(s): 吉本信保、遠山竜也、杉浦博士、遠藤友美、岩佐麻衣、藤井義敬、山下啓子
  • Organizer: 第111回日本外科学会定期学術集会
  • Place of Presentation: 東京
  • Year and Date: 20110526-28
• [Remarks]
  • URL: http://www.med.nagoya-cu.ac.jp/mammal.dir/index.html