Determination of pathogenesis and therapeutic agents in gastric cancer patient with disseminated carcinomatosis of bone marrow

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591703
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kumamoto University
• Principal Investigator: HAYASHI Naoko 熊本大学, 医学部附属病院, 非常勤診療医師 (20452899)
• Co-Investigator(Kenkyū-buntansha): KAMOHARA Hidenobu 熊本大学, 大学院・生命科学研究部, 准教授 (90398222)
• Project Period (FY): 2009 – 2011
• Keywords: 癌幹細胞 / 胃癌骨髄癌腫症 / 遊離癌細胞(ITC)

Research Abstract

We isolated circulating tumor cells(CTCs) using CellSearch^<TM> in five cases of gastric cancer patients with disseminated carcinomatosis of bone marrow. Then, we could identify several candidate genes involved in pathogenesis of this disease, based on the result of gene expression profiling of collected CTCs. As CTCs in two patients remarkably increased and most of CTCs highly expressed HER2, we used' trastuzumab', the monoclonal antibody against HER2, combined with the chemotherapy, against these two patients. They had remission of DIC and long-term survival. Moreover, we could establish mouse xenograft model using CTCs obtained from two gastric cancer patients with disseminated carcinomatosis of bone marrow.

Research Products

• [Journal Article] Usefulness of Transcription-Reverse Transcription Concerted Reaction Method for Detecting Circulating Tumor Cells in Patients With Colorectal Cancer (2012)
  • Author(s): Sato N, Hayashi N, Imamura Y, Tanaka Y, Kinoshita K, Kurashige J, Saito S, Karashima R, Hirashima K, Nagai Y, Miyamoto Y, Iwatsuki M, Baba Y, Watanabe M, Baba H
  • Journal Title: Ann Surg Oncol Volume: (in press)
  • Peer Reviewed
• [Journal Article] The central vein access port and catheter in outpatient chemotherapy for colorectal cancer : a retrospective study of 101 patients (2012)
  • Author(s): Sawayama H, Hayashi N, Watanabe M, Takamori H, Beppu T, Baba H
  • Journal Title: Surg Today Volume: (in press)
  • Peer Reviewed
• [Journal Article] Clinical significance of circulating tumor cells, including cancer stem-like cells, in peripheral blood for recurrence and prognosis in patients with Dukes' stage B and C colorectal cancer (2011)
  • Author(s): Iinuma H, Watanabe T, Mimori K, Adachi M, Hayashi N, Tamura J, Matsuda K, Fukushima R, Okinaga K, Sasako M, Mori M
  • Journal Title: J Clin Oncol Volume: 29 Pages: 1547-1555
  • Peer Reviewed
• [Journal Article] Clinical significance of Wnt-induced secreted protein-1(WISP-1/CCN4) in esophageal squamous cell carcinoma (2011)
  • Author(s): Nagai Y, Watanabe M, Ishikawa S, Karashima R, Kurashige J, Iwagami S, Iwatsuki M, Baba Y, Imamura Y, Hayashi N, Baba H
  • Journal Title: Anticancer Res Volume: 31 Pages: 991-997
  • Peer Reviewed
• [Journal Article] Kyushu Study Group of Clinical Cancer(KSCC): A multicenter phase II clinical study of oxaliplatin, folinic acid, and 5-fluorouracil combination chemotherapy as first-line treatment for advanced colorectal cancer : a Japanese experience (2011)
  • Author(s): Baba H, Hayashi N, Emi Y, Kakeji Y, Egashira A, Oki E, Shirabe K, Toyama T, Ohga T, Yamamoto M, Hasegawa H, Kohakura F, Higashi H, Niwa K, Fujita F, Ogata Y, Kohnoe S, Inomata M, Samura H, Tokunaga S, Maehara Y
  • Journal Title: Surg Today Volume: 41 Pages: 1610-1616
  • Peer Reviewed
• [Journal Article] Laparoscopy assisted resection of an undiagnosed liver tumor and ascending colon cancer via mini median laparotomy : report of a case (2011)
  • Author(s): Mima K, Beppu T, Chikamoto A, Ishiko T, Horino K, Hayashi N, Watanabe M, Takamori H, Okabe K, Yamanaka T, Baba H
  • Journal Title: Surg Today Volume: 41 Pages: 1633-1638
  • Peer Reviewed
• [Journal Article] Reply to B. Faltas et al (2011)
  • Author(s): Iinuma H, Watanabe T, Mimori K, Adachi M, Hayashi N, Tamura J, Nozawa K, Ishihara S, Matsuda K, Fukushima R, Okinaga K, Sasako M, Mori M
  • Journal Title: J Clin Oncol Volume: 29 Pages: 2947-2948
  • Peer Reviewed
• [Journal Article] Reply to F. Crea et al (2011)
  • Author(s): Iinuma H, Watanabe T, Mimori K, Tamura J, Adachi M, Hayashi N, Nozawa K, Ishihara S, Matsuda K, Baba H, Fukushima R, Okinaga K, Sasako M, Mori M
  • Journal Title: J Clin Oncol Volume: 29 Pages: 3488-3490
  • Peer Reviewed
• [Presentation] 消化器癌における微量癌細胞の検出とHER2陽性微量癌細胞を標的とした個別化治療への応用 (2012)
  • Author(s): 岩槻政晃、渡邊雅之、石本崇胤、岩上志朗、馬場祥史、林尚子、豊島功正、林哲、大田義一、馬場秀夫
  • Organizer: 第112回日本外科学会総会
  • Place of Presentation: 千葉幕張メッセ
  • Year and Date: 20120412-14
• [Presentation] 大腸癌イレウスに対する治療法の工夫-経肛門的イレウス管減圧後一期的腹腔鏡下手術の有用性- (2011)
  • Author(s): 林尚子、宮本裕士、齋藤誠哉、田中洋平、蔵重淳二、木下浩一、辛島龍一、平島浩太郎、長井洋平、岩上志朗、岩槻政晃、馬場祥史、渡邊雅之、馬場秀夫
  • Organizer: 第66回日本消化器外科学会総会
  • Place of Presentation: 名古屋名古屋国際会議場
  • Year and Date: 20110713-15
• [Presentation] 切除不能進行再発大腸癌に対する治療戦略-conversion therapyを適切に施行するための指標とは?- (2011)
  • Author(s): 林尚子、佐藤伸隆、増田捻郎、齋藤誠哉、岩上志朗、宮本裕士、岩槻政晃、馬場祥史、近本亮、渡邊雅之、石河隆敏、別府透、馬場秀夫
  • Organizer: 第111回日本外科学会総会
  • Place of Presentation: (紙上発表)
  • Year and Date: 20110500