Prediction of high-risk stage II colorectal cancer by gene expression profile

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591733
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Teikyo University
• Principal Investigator: ISHIHARA Soichiro 帝京大学, 医学部, 講師 (00376443)
• Co-Investigator(Kenkyū-buntansha): WATANABE Toshiaki 帝京大学, 医学部, 教授 (80210920)
• Project Period (FY): 2009 – 2011
• Keywords: 大腸癌 / ハイリスク / 術後補助化学療法 / DNAマイクロアレイ / 遺伝子発現

Research Abstract

Based on the gene expression of stage II colorectal cancer, we aimed to build a predictive model for recurrence after surgery. Using 80 samples as a training set, we examined gene expression by microarray and RT-PCR. We finally selected 8 genes whose expression differed significantly between patients who developed recurrence and who did not. We used these 8 genes for predictive model. The accuracy rate of prediction was 63% among 24 samples as a test set.

Research Products

• [Journal Article] RUNX3 copy number predicts the development of UC-associated colorectal cancer (2011)
  • Author(s): Watanabe T, Kobunai T, Ikeuchi H, Yamamoto Y, Matsuda K, Ishihara S, Nozawa K, Iinuma H, Kanazawa T, Tanaka T, Yokoyama T, Konishi T, Eshima K, Ajioka Y, Hibi T, Watanabe M, Muto T, Nagawa H
  • Journal Title: Int J Oncol Volume: 38(1) Pages: 201-207
  • Peer Reviewed
• [Journal Article] Predicting ulcerative colitis-associated colorectal cancer using reverse-transcription polymerase chain reaction analysis (2011)
  • Author(s): Watanabe T, Kobunai T, Yamamoto Y, Ikeuchi H, Matsuda K, Ishihara S, Nozawa K, Iinuma H, Kanazawa T, Tanaka T, Yokoyama T, Konishi T, Eshima K, Ajioka Y, Hibi T, Watanabe M, Muto T, Nagawa H
  • Journal Title: Clin Colorectal Cancer Volume: 10(2) Pages: 134-141
  • Peer Reviewed
• [Journal Article] Gene expression of vascular endothelial growth factor a, thymidylate synthase, and tissue inhibitor of metalloproteinase 3 in prediction of response to bevacizumab treatment in colorectal cancer patients (2011)
  • Author(s): Watanabe T, Kobunai T, Yamamoto Y, Matsuda K, Ishihara S, Nozawa K, Iinuma H, Shibuya H, Ikeuchi H
  • Journal Title: Dis Colon Rectum Volume: 54(8) Pages: 1026-35
  • Peer Reviewed
• [Journal Article] Differential gene expression signatures between colorectal cancers with and without KRAS mutations : Crosstalk between the KRAS pathway and other signaling pathways (2011)
  • Author(s): Watanabe T, Kobunai T, Yamamoto Y, Matsuda K, Ishihara S, Nozawa K, Iinuma H, Ikeuchi H, Eshima K
  • Journal Title: Eur J Cancer Volume: 47(13) Pages: 1946-1954
  • Peer Reviewed
• [Journal Article] Prognostic Significance of 18q Loss of Heterzygosity in Microsatellite-Stable Colorectal Cancer (2010)
  • Author(s): Watanabe T, Kobunai T, Yamamoto Y, Konishi T, Yano H, Iinuma H, Hayama T, Nozawa K, Ishihara S, Matsuda K
  • Journal Title: J Clin Oncol Volume: 28(7) Pages: e119
  • Peer Reviewed
• [Journal Article] Prediction of liver metastasis after colorectal cancer using reverse transcription-polymerase chain reaction analysis of 10 genes (2010)
  • Author(s): Watanabe T, Kobunai T, Yamamoto Y, Kanazawa T, Konishi T, Tanaka T, Matsuda K, Ishihara S, Nozawa K, Eshima K, Muto T, Nagawa H
  • Journal Title: Eur J Cancer Volume: 46(11) Pages: 2119-26
  • Peer Reviewed
• [Journal Article] Prognostic significance of response to preoperative radiotherapy, lymph node metastasis, and CEA level in patients undergoing total mesorectal excision of rectal cancer (2010)
  • Author(s): Ishihara S, Watanabe T, Kiyomatsu T, Yasuda K, Nagawa H
  • Journal Title: Int J Colorectal Dis Volume: 25(12) Pages: 1417-1425
  • Peer Reviewed
• [Journal Article] Gene expression signature and the prediction of lymph node metastasis in colorectal cancer by DNA microarray (2009)
  • Author(s): Watanabe T, Kobunai T, Tanaka T, Ishihara S, Matsuda K, Nagawa H
  • Journal Title: Dis Colon Rectum Volume: 52(12) Pages: 1941-8
  • Peer Reviewed