2011 Fiscal Year Final Research Report
The molecular functional analysis of micro RNA on the occurrence and development of hepatocellular carcinoma
| Project/Area Number |
21591754
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MAEHARA Yoshihiko 九州大学, 医学研究院, 教授 (80165662)
TAKETOMI Akinobu 九州大学, 大学病院, 講師 (70363364)
IKEGAMI Toru 九州大学, 大学病院, 助教 (80432938)
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| Project Period (FY) |
2009 – 2011
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| Keywords | マイクロRNA / 肝細胞癌 / 肝移植 / マイクロアレイ / リアルタイムPCR |
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| Research Abstract |
The expression of microRNA(miR) on hepatocellular carcinoma(HCC) was comprehensively examined by miR microarray. miR was extracted from 3 HCC cases who underwent liver transplantation. We detected miR-18a which showed high expression in cancerous lesion, and miR-199a-3p which existed in non cancerous liver tissue.
The quantitative expression analysis of miR was done using a real-time PCR method. The miR samples were collected from 70 HCC cases which underwent liver transplantation. The result showed the level of miR-18a was significantly high in cancerous lesion(T/N=1. 8), and miR-199a was significantly deteriorated in cancerous lesion(T/N=0. 31). The higher miR-18a and lower miR-199a were significantly correlated with higher serum AFP and DCP, and larger tumor diameter. Furthermore, those cases showed significantly worse recurrence-free survival rate after surgery.
The molecular function was analyzed in vivo using cultured HCC cells. Target genes of miR-18a, 199awere detected using online database. We examined THFAIP3 ; target gene of miR-18a and HIF1R and cdc42 ; those of miR-199a. The expression of these target genes were significantly inhibited by transfection of the miRs in HCC cells. Our study indicated that miR-18a and miR-199a had important role on the progression of HCC.
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[Presentation] Clinical significance of microRNA expression as a prognostic factor after living donor liver transplantation for hepatocellular carcinoma2011
Author(s)
K Morita, K Shirabe, A Taketomi, T Motomura, Y Mano, K Takeishi, T Toshima, H Uchiyama, T Yoshizumi, Y Maehara
Organizer
6TH International Society of gastroenterological Carcionogenesis(ISGC).
Place of Presentation
Houston, Texas
Year and Date
20110106-08
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[Presentation] MicroRNA-18/199 regulates progression of hepatocellular carcinoma after living donor liver transplantaion2010
Author(s)
K Morita, K Shirabe, A Taketomi, T Motomura, Y Mano, K Takeishi, T Toshima, K Umeda, H Kayashima, M Ninomiya, H Uchiyama, Y Soejima, Y Maehara
Organizer
The 7th International Symposium on Cancer Research and Therapy
Place of Presentation
Tokyo, Japan
Year and Date
20101126-27
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[Presentation] Clinical significance of microRNA expression as a prognostic factor after living donor liver transplantation for hepatocellular carcinoma2010
Author(s)
K Morita, K Shirabe, A Taketomi, T Motomura, Y Mano, K Takeishi, T Toshima, K Umeda, H Kayashima, M Ninomiya, H Uchiyama, Y Soejima, Y Maehara
Organizer
American Society of Clinical Oncology 2010 Gastrointestinal Cancers Symposium : Science and Multidisciplinary Management of GI Malignancies
Place of Presentation
in Orlando, Florida
Year and Date
20100122-24
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