2011 Fiscal Year Final Research Report
Real time measurement of Hydroxylradical following ischemia-reperfusion injury model using rabbit liver.
| Project/Area Number |
21591760
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka Medical College (2011)
Wakayama Medical University (2009-2010) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMAUE Hiroki 和歌山県立医科大学, 医学部, 教授 (20191190)
UENO Masaki 和歌山県立医科大学, 医学部, 助教 (90405465)
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| Project Period (FY) |
2009 – 2011
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| Keywords | Microdialysis / フリーラジカル / 虚血再灌流 / 肝切除術 |
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| Research Abstract |
The aim of this study was to investigate the generation of the hydroxyl radical(・OH) in hepatic tissue in vivo after ischemia/reperfusion(I/R) injury by using a microdialysis technique. Hepatic ischemia was induced in the male rabbit liver by clamping the hepatic artery and portal vein for 20 minutes. Microdialysis probes were placed into the liver perfused with salicylate prior to, during, and after the period of clamping. In anesthetized male rabbits(n=45), ischemia was produced by the selective vascular(hepatic artery and portal vein without caudal lobe branch) occlusion for 20 min, the・OH-specific products of salycylate hydroxylation(2, 3-and 2, 5-dihydroxybenzoic acid : DHBA) in the perfusion fluid from microdalysis into the liver were measured by HPLC equipped with electrochemical detector 9 hours afterwards. There was a significant increase(p<0.05) in the intrahepatic・OH concentrations, compared to the non-ischemic control group, immediately after and seven hours after reperfusion.
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