2011 Fiscal Year Final Research Report
Improving safety in neural celltransplantation with human inducedpluripotent stem cells
| Project/Area Number |
21591837
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kyoto University |
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Principal Investigator |
MORIZANE Asuka 京都大学, ips細胞研究所, 特定研究員 (10528730)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAHASHI Jun 京都大学, 再生医科学研究所・ips細胞研究所, 准教授 (10270779)
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| Project Period (FY) |
2009 – 2011
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| Keywords | パーキンソン病 / パーキンソン病 / 腫瘍化 / 神経 / 多能性幹細胞 / ソーティング / ドーパミン / 移植 |
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| Research Abstract |
We found and reported several strategies for improving safety foriPSC-derived neurons as donor cells : the usage of two small molecules for inhibiting BMPand Activin cascade, prolonged culture for differentiation and inhibition of Notchsignals in vitro before transplantation. After differentiation of the donor cells withthese methods, a good graft survival of iPSC-derived dopaminergic neurons was observedin the brain of a primate model of Parkinson' sdisease. We demonstrated that the humaniPSCs established with episomal vectors having no genomic insertion of transgenescould generate dopaminergic neurons as well as original retrovirally establishediPSCs or embryonic stem cells.
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