Gene transfection with endogenous opioid in the intractable pain models

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591906
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Teikyo University
• Principal Investigator: OZAWA Tomoyuki 帝京大学, 医学部, 講師 (60375706)
• Co-Investigator(Kenkyū-buntansha): TOYONE Tomoaki 帝京大学, 医学部, 教授 (10407918) ; OOTORI Seiji 千葉大学, 医学部付属病院, 助教 (40361430)
• Project Period (FY): 2009 – 2011
• Keywords: 難治性疼痛 / 内因性オピオイド / 体外衝撃波 / 遺伝子導入 / 神経障害性疼痛 / 椎間板性腰痛 / proopiomelanocortin / 炎症性疼痛

Research Abstract

This study investigated the effects of POMC(proopiomelanocortin, the precursor forβ-endorphin, an endogenous opioid) gene transfection using radial shock waves(RSWs). We used three models ; inflammatory pain model, neuropathic pain model, and discogenic low back pain model. POMC gene transfection suppressed CGRP expression in the DRGs, microglial activation in the dorsal horn of the spinal cord, and pain behavior These findings suggest the effectiveness of POMC gene transfection using RSWs for the treatment of inflammatory pain, neuropathic pain, and discogenic low back pain, without side effects for a long time.

Research Products

• [Presentation] 脳内モルヒネであるエンドルフィンの遺伝子導入により神経障害性疼痛が抑制される (2011)
  • Author(s): 佐久間詳浩、石川哲大、宮城正行、大鳥精司、井上玄、鴨田博人、新井玄、鈴木都、及川泰宏、高橋和久
  • Organizer: 第4回日本運動器疼痛学会、4、ライフサイエンスセンタービル
  • Place of Presentation: 大阪
  • Year and Date: 20111100
• [Presentation] In vivo transfection of proopiomelanocortin gene by radial shock waves alleviates neuropathic pain (2011)
  • Author(s): Ishikawa T, Hashimoto M, Kamoda H, Miyagi M, Arai G, Murakami K, Suzuki M, Sakuma Y, OikawaY, Inoue G, Ohtori S, Takahashi K
  • Organizer: Neuroscience 2011
  • Place of Presentation: Washington D. C., USA
  • Year and Date: 20111100
• [Presentation] Gene transfection of mouse cells with precursor of endogenous endorphin in mouse cancer pain model (2011)
  • Author(s): Tetsuhiro Ishikawa
  • Organizer: IMUST/RCAST-Chiba University Global COE Joint Retreat 2011. "Toward new era in the basic and clinical immunology"
  • Place of Presentation: Oiso Prince Hotel
  • Year and Date: 20110900
• [Presentation] Transfection of rat cells with proopiomelanocortin gene using radial shock waves alleviates neuropathic pain (2011)
  • Author(s): Tetsuhiro Ishikawa, Seiji Ohtori, Masayuki Miyagi, Hiroto Kamoda, Gen Inoue, Kazuhisa Takahashi
  • Organizer: 66th Annual Meeting of the American Society for Surgery of the Hand
  • Place of Presentation: Las Vegas, USA
  • Year and Date: 20110900
• [Presentation] Transfection of rat cells with proopiomelanocortin gene using radial shock waves alleviates neuropathic pain (2011)
  • Author(s): Ishikawa T, Yamashita M, Miyagi M, Ohtori S, Inoue G, Eguchi Y, Kamoda H, Arai G, Suzuki M, Kubota G, Sakuma Y, Oikawa Y, Takahashi K
  • Organizer: 38th International Society for the Study of the Lumbar Spine
  • Place of Presentation: Gothenburg, Sweden
  • Year and Date: 20110600
• [Presentation] Transfection of rat cells with proopiomelanocortin gene using radial shock waves alleviates neuropathic pain (2011)
  • Author(s): Ishikawa T, Yamashita M, Miyagi M, Ohtori S, Inoue G, Eguchi Y, Kamoda H, Arai G, Suzuki M, Kubota G, Sakuma Y, Oikawa Y, Takahashi K
  • Organizer: The 8th Combined Congress of the Spine and Pediatric Sections, Asia Pacific Orthopaedic Association
  • Place of Presentation: Gifu, Japan
  • Year and Date: 20110600
• [Presentation] 内因性オピオイド前駆物質プロオピオメラノコルチン遺伝子の体外衝撃波による遺伝子導入はラット神経障害性疼痛を抑制する (2011)
  • Author(s): 石川哲大、山下正臣、宮城正行、大鳥精司、井上玄、折田純久、江口和、鴨田博人、新井玄、鈴木都、久保田剛、佐久間詳浩、及川泰宏、高橋和久
  • Organizer: 第40回日本脊椎脊髄病学会
  • Place of Presentation: 京王プラザホテル東京
  • Year and Date: 20110400
• [Presentation] 腰椎椎間板傷害モデルに対する内因性エンドルフィン前駆物質遺伝子導入による鎮痛効果の検討 (2011)
  • Author(s): 及川泰宏、石川哲大、山下正臣、大鳥精司、井上玄、江口和、鴨田博人、宮城正行、新井玄、鈴木都、久保田剛、佐久間詳浩、高橋和久
  • Organizer: 第40回日本脊椎脊髄病学会
  • Place of Presentation: 京王プラザホテル東京
  • Year and Date: 20110400