2011 Fiscal Year Final Research Report
Osteogenic differentiation of culture expanded bone marrow cells under tensile and compressive strains
Project/Area Number |
21591915
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | Nagoya University |
Principal Investigator |
KITOH Hiroshi 名古屋大学, 医学部附属病院, 講師 (40291174)
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Co-Investigator(Kenkyū-buntansha) |
KANEKO Hiroshi 名古屋大学, 大学院・医学系研究科, 講座助教 (60566975)
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Project Period (FY) |
2009 – 2011
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Keywords | メカニカルストレス / 骨髄細胞培養 / 骨組織再生 / 骨延長モデル / 多血小板血漿 / HIF 1α / 三次元培養 |
Research Abstract |
Alkaline phosphatase activity within the culture media were significantly elevated in the dynamic stretch(DS) group and the dynamic compression(DC) group compared to the control group. The DC group showed significant elevated levels of mRNA expression of HIF 1α. Western blotting analysis demonstrated increased amount of HIF 1αprotein within the nucleous in the DC, DS, and static stretch(SS) groups. Favorable new bone formation was observed when the bone marrow cells cultured under the dynamic stretch condition were implanted with PRP into the distraction gap of rat bone lengthening models.
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Research Products
(34 results)
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[Journal Article] Mutations in the TGFβbinding protein-like domain 5 of FBN1 are responsible acromicric and geleo physicdys plasias2011
Author(s)
Le Goff C, Mahaut C, Wang LW, Allali S, Abhyankar A, Jensen S, Zilberberg L, Collod-Beroud G, Bonnet D, Alanay Y, Brady AF, Cordier MP, Devriendt K, Genevieve D, Kiper POS, Kitoh H, Krakow D, Lynch SA, Le Merrer M, Megarbane1A, Mortier G, Odent S, Polak M
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Journal Title
Am J Hum Genet
Volume: 89(1)
Pages: 7-14
Peer Reviewed
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[Journal Article] A novel mutation of ALK2, L196P, found in the most benign case of fibrodysplasia ossificans progressiva activates BMP-specific intracellular signaling equivalent to a typical mutation, R206H2011
Author(s)
Ohte S, Shin M, Sasanuma H, Yoneyama K, Akita M, Ikebuchi K, Jimi E, Maruki Y, Matsuoka M, Miyazono K, Nanba A, Tomoda H, Okazaki Y, Ohtake A, Oda H, Owan I, Yoda T, Furuya H, Kamizono J, Kitoh H, Nakashima Y, Susami T, Haga N, Komori T, Katagiri T.
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Journal Title
Biochem Biophys Res Commun
Volume: 407(1)
Pages: 213-8
Peer Reviewed
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[Journal Article] Novel and recurrentTRPV4 mutations and their association with distinct phenotypes within the TRPV4dysplasia family2010
Author(s)
Dai J, Kim OH, Cho TJ, Schmidt-Rimpler M, Tonoki H, Takikawa K, Haga N, Miyoshi K, Kitoh H, Yoo WJ, Choi IH, Song HR, Jin DK, Kim HT, Kamasaki H, Bianchi P, Grigelioniene G, Nampoothiri S, Minagawa M, Miyagawa S, Fukao T, Marcelis C, Jansweijer MCE, Hennekam RCM, Bedeschi F, Mustonen A, Jiang Q, Ohashi H, Furuichi T, Unger S, Zabel B, Lausch E, Superti-Furga A, Nishimura N, Ikegawa S.
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Journal Title
J Med Genet
Volume: 47(10)
Pages: 704-709
Peer Reviewed
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[Journal Article] Spondylo-epiphyseal dysplasia, Maroteaux type(pseudo-Morquio syndrome type 2) and parastremmatic dysplasia are caused byTRPV4 mutations2010
Author(s)
Nishimura G, Dai J, Lausch E, Unger S, Megarbane A, Kitoh H, OH Kim, Cho TJ, Bedeschi F, Benedicenti F, Mendoza-Londono R, Silengo M, Schmidt-Rimpler M, Spranger J, Zabel B, kegawa S, Superti-Furga A.
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Journal Title
Am J Med Genet
Volume: 152A(6)
Pages: 1443-1449
Peer Reviewed
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