Exploration of a new molecular target of hormone refractory prostate cancer based on nuclear matrix proteins

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592043
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kyoto University
• Principal Investigator: INOUE Takahiro 京都大学, 医学研究科, 助教 (80511881)
• Co-Investigator(Kenkyū-buntansha): OGAWA Osamu 京都大学, 医学研究科, 教授 (90260611)
• Project Period (FY): 2009 – 2011
• Keywords: PURA LASP-1 / 前立腺癌 / 去勢抵抗性

Research Abstract

The aim of this research is to analyze whether a nuclear matrix protein, Purine-element binding protein alpha(PURA), is useful for exploration for a new molecular target of hormone refractory prostate cancer.
Overexpression of PURA obviously lowered the growth speed of PC3 cells in vivo, and by immunohistochemistry, Ki67 expression was weaker in PC3 cells with PURA overexpression than in control PC3 cells. And we have established tissue microarray, including 22 cases of castration resistant prostate cancer, which were obtained by TUR. Using this microarray, we are planning to ask how those expression changes with progression of prostate cancer.

Research Products

• [Journal Article] Roleof Signaling Transduction Pathways inDevelopment of Castration-ResistantProstate Cancer (2011)
  • Author(s): Takahiro Inoue, Osamu Ogawa
  • Journal Title: Prostate Cancer Volume: 7 Pages: 647987
  • DOI: doi:10.1155/2011/647987.2011
• [Journal Article] Long-termoutcomes of three-dimensional conformalradiation therapy combined with neoadjuvant hormonal therapy for Japanesepatients with T1c-T2N0M0 prostatecancer (2011)
  • Author(s): Mizowaki T, Takayama K, Norihisa Y, Ogura M, Kamba T, Inoue T, Shimizu Y, Kamoto T, Ogawa O, Hiraoka M.
  • Journal Title: Int J Clin Oncol Volume: 5
  • DOI: doi:10.1007/s10147-011-0326-z
• [Journal Article] A potent chemotherapeutic strategy inprostate cancer : S-(methoxytrityl)-l-cysteine, a novel Eg5 inhibitor (2011)
  • Author(s): Xing ND, Ding ST, Saito R, Nishizawa K, Kobayashi T, Inoue T, Oishi S, Fujii N, Lv JJ, Ogawa O, Nishiyama H
  • Journal Title: Asian J Androl Volume: 13 Pages: 236-41
  • DOI: doi:10.1038/aja.2010.171
• [Journal Article] SPA-1 controls the invasionand metastasis of human prostate cancer (2011)
  • Author(s): Shimizu Y, Hamazaki Y, Hattori M, Doi K, Terada N, Kobayashi T, Toda Y, Yamasaki T, Inoue TA, Kajita Y, Maeno A, Kamba T, Mikami Y, Kamoto T, Yamada T, Kanno T, Yoshikawa K, Ogawa O, Minato N, Nakamura E
  • Journal Title: Cancer Sci Volume: 102 Pages: 828-36
  • DOI: doi:10.1111/j.1349-7006.2011.01876.x
• [Journal Article] Activationof Rac1 Is Closely Related toAndrogen-Independent Cell Proliferationof Prostate Cancer Cells Both in Vitro andin Vivo (2010)
  • Author(s): Kobayashi T, Inoue T, Shimizu Y, Terada N, Maeno A, Kajita Y, Yamasaki T, Kamba T, Toda Y, Mikami Y, Yamada T, Kamoto T, Ogawa O, Nakamura E
  • Journal Title: Mol Endocrinol Volume: 24 Pages: 722-34
  • DOI: doi:10.1210/me.2009-0326
• [Journal Article] Identification of EP4as a Potential Target for the Treatment ofCastration-Resistant Prostate CancerUsing a Novel Xenograft Model (2010)
  • Author(s): Terada N, Shimizu Y, Kamba T, Inoue T, Maeno A, Kobayashi T, Nakamura E, Kamoto T, Kanaji T, Maruyama T, Mikami Y, Toda Y, Matsuoka T, Okuno Y, Tsujimoto G, Narumiya S, Ogawa O
  • Journal Title: Cancer Res Volume: 70 Pages: 1606-15
  • DOI: doi:10.1158/0008-5472.CAN-09-2984
• [Journal Article] Regulation ofandrogen receptor transactivity andmTOR-S6 kinase pathway by Rheb in prostatecancer cell proliferation (2010)
  • Author(s): Takashi Kobayashi, Yosuke Shimizu, Naoki Terada, Toshinari Yamasaki, Eijiro Nakamura, Yoshinobu Toda, Hiroyuki Nishiyama, Toshiyuki Kamoto, Osamu Ogawa and, Takahiro Inoue
  • Journal Title: Prostate Volume: 70 Pages: 866-874
  • DOI: doi:10.1002/pros.21120
• [Journal Article] Osamu OgawaAntiandrogen Withdrawal Syndrome andAlternative Antiandrogen TherapyAssociated with the W741C Mutant AndrogenReceptor in a Novel Prostate CancerXenograft (2009)
  • Author(s): Naoki Terada, Yosuke Shimizu, Toru Yoshida, Atsushi Maeno, Tomomi Kamba, Takahiro Inoue, Eijiro Nakamura, Toshiyuki Kamoto
  • Journal Title: Prostate Volume: 70 Pages: 252-261
  • DOI: doi:10.1002/pros.21058
• [Journal Article] Restorationof cyclin D2 has an inhibitory potentialon the proliferation of LNCaP cells (2009)
  • Author(s): Kobayashi T, Nakamura E, Shimizu Y, Terada N, Maeno A, Kobori G, Kamba T, Kamoto T, Ogawa O, Inoue T
  • Journal Title: Biochem Biophys Res Commun Volume: 387(1) Pages: 196-201
  • DOI: 10.1016/j.bbrc.2009.06.146
• [Journal Article] Down-regulating cold shockprotein genes impairs cancer cell survivaland enhances chemosensitivity (2009)
  • Author(s): Zeng Y, Kulkarni P, Inoue T, Getzenberg RH.
  • Journal Title: J CellBiochem Volume: 107 Pages: 179-88
  • DOI: doi:10.1002/jcb.22114
• [Journal Article] Purine-rich elementbinding protein(PUR) alpha inducesendoplasmic reticulum stress response, and cell differentiation pathways inprostate cancer cells (2009)
  • Author(s): Inoue T, Maeno A, Talbot C Jr, Zeng Y, Yeater DB, Leman ES, Kulkarni P, Ogawa O, Getzenberg RH.
  • Journal Title: Prostate Volume: 69 Pages: 861-73
  • DOI: doi:10.1002/pros.20936
• [Remarks]
  • URL: http://www.urology.kuhp.kyoto-u.ac.jp/