Identification of gene signatures associated with renal tumor characteristics and its clinical applications

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592053
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Yokohama City University
• Principal Investigator: YAO Masahiro 横浜市立大学, 医学研究科, 准教授 (00260787)
• Co-Investigator(Kenkyū-buntansha): NAKAIGAWA Noboru 横浜市立大学, 医学研究科, 准教授 (00237207) ; NAGASHIMA Yoji 横浜市立大学, 医学研究科, 准教授 (10217995)
• Project Period (FY): 2009 – 2011
• Keywords: 腎細胞癌 / 遺伝子発現 / 予後

Research Abstract

Renal cell carcinomas(RCCs) are morphologically and genetically heterogeneous tumors and present diverse clinical courses. We selected differentially expressed gene-set between metastasis-positive and-negative phenotype from the DNA microarray data. We then focus on the three genes(AQP1, PLTP, and PTHLH) and confirmed their expression levels using quantitative reverse transcriptase PCR in more than 500 renal tumors. Each of the three genes presented unique expression patterns according to the renal tumor histology. We also demonstrated that each gene is tightly associated with clear cell RCC patient survivals. These data suggested that the three genes provide useful diagnostic and prognostic information for patients with RCC.

Research Products

• [Journal Article] Genome-wide methylation analysis identifies epigenetically inactivated candidate tumour suppressor genes in renal cell carcinoma (2011)
  • Author(s): Morris MR, Ricketts CJ, Gentle D, McRonald F, Carli N, Khalili H, Brown M, Kishida T, Yao M, Banks RE, Clarke N, Latif F, Maher ER
  • Journal Title: Oncogene Volume: 30 Pages: 1390-1401
• [Journal Article] Copy number profiling in von Hippel-Lindau disease renal cell carcinoma (2011)
  • Author(s): Shuib S, Wei W, Sur H, Morris MR, McMullan D, Rattenberry E, Meyer E, Maxwell P, Kishida T, Yao M, Latif F, Maher ER
  • Journal Title: Genes Chromosomes Cancer Volume: 50 Pages: 479-882
• [Journal Article] 【腎細胞癌の分子標的治療】腎細胞癌の分子標的治療の理論的根拠(解説/特集) (2011)
  • Author(s): 中井川昇, 矢尾正祐
  • Journal Title: 医学のあゆみ Volume: 236 Pages: 1085-1089
• [Journal Article] (Session1)疫学、診断(画像、病理、分子発生学)、予後因子腎癌分子発生学 (2011)
  • Author(s): 矢尾正祐, 中井川昇, 長嶋洋治, 岸田健
  • Journal Title: 泌尿器外科 Volume: 24 Pages: 715-716
• [Journal Article] Identification of candidate tumour suppressor genes frequently methylated in renal cell carcinoma (2010)
  • Author(s): Morris MR, Ricketts C, Gentle D, Abdulrahman M, Clarke N, Brown M, Kishida T, Yao M, Latif F, Maher ER
  • Journal Title: Oncogene Volume: 29 Pages: 2104-2117
• [Journal Article] Molecular genetic events associated with renal cell carninoma and its implication to treatment by molecular target therapy (2010)
  • Author(s): Kishida T, Yao M, Uemura H, Ohlmann C, Tomita Y, Bukowski RM, Naito S
  • Journal Title: Int J Urol Volume: 17 Pages: 198-205
• [Journal Article] 腎癌の病理分類 (2010)
  • Author(s): 長嶋洋治, 稲山嘉明, 青木一郎, 矢尾正祐
  • Journal Title: 日本臨床 Volume: 68 Pages: 36-44
• [Journal Article] 腎癌の癌遺伝子・癌抑制遺伝子 (2010)
  • Author(s): 矢尾正祐, 近藤慶一, 中井川昇
  • Journal Title: 日本臨床 Volume: 68 Pages: 25-29
• [Journal Article] 【病理形態学キーワード】腎乳頭状構築 (2010)
  • Author(s): 長嶋洋治, 小林志津子, 村上あゆみ, 青木一郎, 矢尾正祐
  • Journal Title: 病理と臨床 Volume: 28 Pages: 202-203
• [Journal Article] 小径腎腫瘍の診断と治療Update (2010)
  • Author(s): 長嶋洋治, 青木一郎, 稲山嘉明, 矢尾正祐
  • Journal Title: 小径腎腫瘍の病理診断(解説/特集) Volume: 84 Pages: 15-20
• [Journal Article] 【腎腫瘍up to date 2010s】腎腫瘍の分子生物学 (2010)
  • Author(s): 矢尾正祐, 中井川昇, 近藤慶一, 長嶋洋治
  • Journal Title: 病理と臨床 Volume: 28 Pages: 1037-1042
• [Journal Article] 【腎腫瘍up to date 2010s】乳頭状腎細胞癌と集合管癌 (2010)
  • Author(s): 長嶋洋治, 青木一郎, 稲山嘉明, 矢尾正祐, 松嵜理
  • Journal Title: 病理と臨床 Volume: 28 Pages: 1048-1052
• [Journal Article] Expression of Aquaporin 1 in primary renal tumors : A prognostic indicator for clear-cell renal cell carcinoma (2009)
  • Author(s): Huang Y, Murakami T, Sano F, Kondo K, Nakaigawa N, Kishida T, Kubota Y, Nagashima Y, Yao M.
  • Journal Title: Eur Urol Volume: 56 Pages: 690-698
• [Journal Article] Epithelioid angiomyolipoma of the kidney (2009)
  • Author(s): Kato I, Inayama Y, Yamanaka S, Ohshiro H, Gomi K, Shirai S, Aoki I, Uemura H, Miyoshi Y, Kubota Y, Yao M, Nagashima Y
  • Journal Title: Pathol Int Volume: 59 Pages: 38-43
• [Journal Article] CpG methylation profiling in VHL related and VHL unrelated renal cell carcinoma (2009)
  • Author(s): McRonald FE, Morris MR, Gentle D, Winchester L, Baban D, Ragoussis J, Clarke NW, Brown MD, Kishida T, Yao M, Latif F, Maher ER
  • Journal Title: Mol Cancer Volume: 8(31)
• [Journal Article] 【腎における細胞内シグナル伝達と病態生理】腎細胞癌におけるシグナル伝達 (2009)
  • Author(s): 中井川昇, 矢尾正祐, 窪田吉信
  • Journal Title: 腎と透析 Volume: 67 Pages: 387-381
• [Journal Article] 【泌尿器画像診断update】腎腫瘍の病理(解説/特集) (2009)
  • Author(s): 長嶋洋治, 古林直人, 加川絵理子, 青木一郎, 稲山嘉明, 矢尾正祐
  • Journal Title: 臨床画像 Pages: 6-18
• [Presentation] 腎腫瘍におけるPTHLH遺伝子の発現解析:PTHLHは淡明細胞型腎癌に特異的な発現・予後マーカーである (2012)
  • Author(s): 矢尾正祐, 村上貴之, 黄エイ, 水野伸彦、佐野太, 渕桂子, 槙山和秀, 中井川昇, 塩井康一, 岸田健, 長嶋洋治, 窪田吉信
  • Organizer: 第100回日本泌尿器科学会総会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2012-04-23
• [Presentation] 腎癌におけるPLTP遺伝子の発現と臨床病理・予後との関係 (2011)
  • Author(s): 矢尾正祐, 黄エイ, 長嶋洋治, 佐野太, 近藤慶一, 槙山和秀, 中井川昇, 塩井康一, 岸田健, 窪田吉信
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 名古屋国際会議場(愛知)
  • Year and Date: 2011-04-22
• [Presentation] Expression analysis of Phospholipid Transfer Protein in Clear Cell Renal Carcinoma (2010)
  • Author(s): Huang Y, Murakami T, Sano F, Kondo K, Nakaigawa N, Kishida K, Kubota Y, Nagashima Y, Yao M.
  • Organizer: 第69回日本癌学会総会(英文)
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2010-09-24
• [Presentation] 遺伝子発現からみた腎癌の新たな分類 (2010)
  • Author(s): 矢尾正祐
  • Organizer: 第98回日本泌尿器科学会総会
  • Place of Presentation: 盛岡市民文化ホール(岩手)(教育講演)
  • Year and Date: 2010-04-29
• [Presentation] 転移性腎癌治療:病理組織型、遺伝子型からの俯瞰 (2009)
  • Author(s): 矢尾正祐、蓮見壽史、村上貴之、槇山和秀、近藤慶一、中井川昇、長嶋洋治、窪田吉信
  • Organizer: 第47回日本癌治療学会学術大会
  • Place of Presentation: パシフィコ横浜(神奈川)(ワークショップ)
  • Year and Date: 2009-10-23
• [Presentation] 淡明細胞腎癌の予後と相関する発現遺伝子群の同定と予後予測スコアモデルの作成 (2009)
  • Author(s): 黄エイ, 村上貴之, 佐野太, 近藤慶一, 中井川昇, 岸田健, 窪田吉信, 長嶋洋治, 矢尾正祐
  • Organizer: 第68回日本癌学会総会(英文)
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2009-10-02
• [Presentation] 腎癌におけるAquapolin 1遺伝子発現とその診断有用性、臨床応用についての検討 (2009)
  • Author(s): 矢尾正祐, 黄エイ, 村上貴之, 佐野太, 近藤慶一, 中井川昇, 岸田健, 長嶋洋治, 窪田吉信
  • Organizer: 第97回日本泌尿器科学会総会
  • Place of Presentation: 岡山コンベンションセンター(岡山)
  • Year and Date: 2009-04-17