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2011 Fiscal Year Final Research Report

In vitro system for spermatogenesis from spermatogonial stem cells

Research Project

  • PDF
Project/Area Number 21592080
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionYokohama City University

Principal Investigator

OGAWA Takehiko  横浜市立大学, 医学部, 准教授 (50254222)

Co-Investigator(Kenkyū-buntansha) YUMURA Yasushi  横浜市立大学, 市民総合医療センター, 講師 (30522023)
Project Period (FY) 2009 – 2011
Keywords精子形成 / 精子幹細胞 / 培養
Research Abstract

In vitro reconstitution of spermatogenesis is a very difficult task and remained unsuccessful for a long time. We challenged that task using classical organ culture method, called air-liquid interphase. Through testing variety of culture conditions, we found that a serum replacement, KSR, is effective and induced complete spermatogenesis of mouse. Offspring were obtained with the sperm produced in vitro. We also showed that cryopreservation of testis tissue fragment is possible to preserve spermatogenic ability of the tissues.

  • Research Products

    (16 results)

All 2012 2011 2010 2009

All Journal Article (5 results) Presentation (11 results)

  • [Journal Article] In vitro production of fertile sperm from murine spermatogonial stem cell lines2011

    • Author(s)
      Sato T, Katagiri K, Yokonishi T, Kubota Y, Inoue K, Ogonuki N, Matoba S, Ogura A, Ogawa T
    • Journal Title

      Nat. Commun

      Volume: 2 Pages: 472

  • [Journal Article] In vitro production of functional sperm in cultured neonatal mouse testes2011

    • Author(s)
      Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, Ogura A, Kubota Y, Ogawa T
    • Journal Title

      Nature

      Volume: 471 Pages: 504-507

  • [Journal Article] Proliferation of mouse spermatogonial stem cells in microdrop culture2010

    • Author(s)
      Araki Y, Sato T, Katagiri K, Kubota Y, Araki Y, Ogawa T
    • Journal Title

      Biology of Reproduction

      Volume: 83 Pages: 951-957

  • [Journal Article] In vitro murine spermatogenesis in an organ culture system2010

    • Author(s)
      Gohbara A, Katagiri K, Sato T, Kubota Y, Kagechika H, Araki Y, Araki Y, Ogawa T
    • Journal Title

      Biology of Reproduction

      Volume: 83 Pages: 261-267

  • [Journal Article] Effect of Reactive Oxygen Species in Semen on the Pregnancy of Infertile Couples2009

    • Author(s)
      Yumura Y, Iwasaki A, Saito K, Ogawa T, Hirokawa M
    • Journal Title

      Int. J. Urology

      Volume: 16 Pages: 202-207

  • [Presentation] 器官培養法を用いたin vitro精子形成法の開発2012

    • Author(s)
      小川毅彦
    • Organizer
      第20回平成不妊研究会(招待講演)
    • Place of Presentation
      六本木・アカデミーヒルズ(東京都)
    • Year and Date
      2012-03-15
  • [Presentation] 器官培養法を用いたin vitro精子形成法の開発2011

    • Author(s)
      小川毅彦
    • Organizer
      第26回動物生殖工学研究会(招待講演)
    • Place of Presentation
      北里大学・北里本館2F大会議室(東京都)
    • Year and Date
      2011-12-04
  • [Presentation] 器官培養法を用いた精子形成法の開発と臨床応用の可能性2011

    • Author(s)
      小川毅彦
    • Organizer
      第2回生殖医療研究会(招待講演)
    • Place of Presentation
      国際医療福祉大学青山キャンパス(東京都)
    • Year and Date
      2011-11-06
  • [Presentation] 器官培養法を用いたin vitro精子形成法の開発と発展2011

    • Author(s)
      小川毅彦
    • Organizer
      第89回発生工学・疾患モデル研究会(招待講演)
    • Place of Presentation
      順天堂大学(東京都)
    • Year and Date
      2011-10-26
  • [Presentation] 器官培養法によるin vitro精子形成と臨床応用の可能性2011

    • Author(s)
      小川毅彦
    • Organizer
      第76回日本泌尿器科学会東部総会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-10-22
  • [Presentation] In vitro spermatogenesis2011

    • Author(s)
      Ogawa T
    • Organizer
      57th Annual meeting of Canadian Fertility and Andrology Society(Invited)
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2011-09-21
  • [Presentation] マウス精巣の器官培養によるin vitro精子形成シンポジウム「精原幹細胞:基礎から応用まで」2011

    • Author(s)
      小川毅彦
    • Organizer
      第104回日本繁殖学会(招待講演)
    • Place of Presentation
      いわて県民情報交流センター・アイーナ(岩手県)
    • Year and Date
      2011-09-17
  • [Presentation] In vitro production of functional sperm in cultured neonatal mouse testes2011

    • Author(s)
      Ogawa T
    • Organizer
      16th World Congress on In vitro Fertilization & 5th World Congress on In vitro Maturation(Invited)
    • Place of Presentation
      Keio Plaza, Tokyo, Japan
    • Year and Date
      2011-09-13
  • [Presentation] 器官培養法を用いた新生仔マウス精巣でのin vitro精子産生2011

    • Author(s)
      小川毅彦、佐藤卓也、横西哲広、郷原絢子、河路かおる、窪田吉信
    • Organizer
      第99回日本泌尿器科学会総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-04-22
  • [Presentation] In vitro精子形成の進展2011

    • Author(s)
      小川毅彦
    • Organizer
      第6回日本生殖再生医学会
    • Place of Presentation
      東京、シェーンバッハ・サボー
    • Year and Date
      2011-03-13
  • [Presentation] 精子幹細胞の培養とin vitro精子形成の進展/シンポジウム「精子形成研究の新たなるフロンティア:幹細胞から精子の品質まで」2010

    • Author(s)
      小川毅彦
    • Organizer
      第55回日本生殖医学会総会
    • Place of Presentation
      徳島市あわぎんホール
    • Year and Date
      2010-11-12

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Published: 2013-07-31  

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