Pathogenesis of endometriosis by the inflammatory cytokines in three-dimension culture system

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592097
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Tottori University
• Principal Investigator: IWABE Tomio 鳥取大学, 医学部, 准教授 (10284001)
• Co-Investigator(Kenkyū-buntansha): DEURA Imari 鳥取大学, 医学部附属病院, 助教 (50464293)
• Project Period (FY): 2009 – 2011
• Keywords: 子宮内膜症 / 子宮内膜 / 炎症性サイトカイン / 3次元培養系

Research Abstract

1. We examined the effect of menstrual blood on the ability of cytokine production in these immune cells. We examined the CD14+monocyte and DC were cultured the containing 2% or 10% menstrual blood in the culture medium for 24 hours, and measured the concentration of interleukin-6 and interleukin-8 in the supernatants with ELISA. We demonstrated that the menstrual blood induced the cytokine levels in the immune cells. These results suggest that retrograde menstruation may be contributed to the pathogenesis of endometriosis.
2 We examined the three-dimensional culture system. Endometrial and endometriotic stromal cells were obtained from normal endometrium and from chocolate cyst linings. We used Celltight X TM(SUMILON). We evaluate the effect of TNFa on those calls compared with monolayer culture systems. IL-6 and IL-8 protein production was examined by ELISA. Endometriotic stromal cells product the inflammatory cytokines without TNFa. The addition of TNFa promoted the production of inflammatory cytokines, compared with monolayer culture systems. These results suggested that the inflammatory cytokines work as the autocrine and paracrine factor for the proliferation of endometriotic cells

Research Products

• [Journal Article] The role of NF-kappaB in endometriosis (2012)
  • Author(s): Kaponis A, Iwabe T, Taniguchi F, Ito M, Deura I, Decavalas G, Terakawa N, Harada T.
  • Journal Title: Front Biosci Volume: 4 Pages: 1213-34
• [Journal Article] Apoptosis and endometriosis (2011)
  • Author(s): Taniguchi F, Kaponis A, Izawa M, Kiyama T, Deura I, Ito M, Iwabe T, Adonakis G, Terakawa N, Harada T
  • Journal Title: Front Biosci Volume: 3 Pages: 648-62
• [Journal Article] Demethylation of a nonpromoter cytosine-phosphate-guanine island in the aromatase gene may cause the aberrant up-regulation in endometriotic tissues (2011)
  • Author(s): Izawa M, Taniguchi F, Uegaki T, Takai E, Iwabe T, Terakawa N, Harada T
  • Journal Title: Fertil Steril Volume: 95 Pages: 33-9
• [Journal Article] TAK1 activation for cytokine synthesis and proliferation of endometriotic cells (2009)
  • Author(s): Taniguchi F, Harada T, Miyakoda H, Iwabe T, Deura I, Tagashira Y, Miyamoto A, Watanabe A, Suou K, Uegaki T, Terakawa N
  • Journal Title: Mol Cell Endocrinol Volume: 307 Pages: 196-204
• [Presentation] Aberrant expression of IAP family in endometriotic cells may cause resistance to apoptosis (2011)
  • Author(s): Taniguchi F, Izawa M, T. Uegaki, Iwabe T, Terakawa N, Harada T
  • Organizer: 11th World Congress on Endometriosis
  • Place of Presentation: Montpellier
  • Year and Date: 2011-09-06
• [Presentation] 子宮内膜症 (2010)
  • Author(s): 岩部富夫
  • Organizer: 第55回日本生殖医学会総会・学術講演会
  • Place of Presentation: ワークショップ徳島
  • Year and Date: 2010-11-11
• [Presentation] Advanced Techniques of Adhesiolysis for Severe Endometriosis (2010)
  • Author(s): Deura I, Kaponis A, Ito Mu, Taniguchi F, Iwabe T, Harada T
  • Organizer: 39th Global Congress of Minimally Invasive Gynecology
  • Place of Presentation: Las Vegas
  • Year and Date: 2010-11-10
• [Presentation] Aberrant expression of IAP family in endometriotic cells may cause resistance to apoptosis (2010)
  • Author(s): Taniguchi F, Izawa M, Iwabe T, Terakawa N、Harada T
  • Organizer: 66th Annual Meeting of American Society for Reproductive Medicine
  • Place of Presentation: Denver
  • Year and Date: 2010-10-28
• [Presentation] Apigenin inhibits TNFα-induced cell proliferation in endometriotic stromal cells (2009)
  • Author(s): Taniguchi F, Tagashira Y, Suou K, Iwabe T, Harada T
  • Organizer: 65^<th> Annual Meeting of American Society for Reproductive Medicine
  • Place of Presentation: Atlanta
  • Year and Date: 2009-10-20