Melatonin protects oocyte and granulosa cellsfrom reactive oxygen species during ovulation process within the follicle

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592099
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Yamaguchi University
• Principal Investigator: TAMURA Hiroshi 山口大学, 医学部附属病院, 准教授 (50379947)
• Co-Investigator(Kenkyū-buntansha): TAKETANI Toshiaki 山口大学, 医学部附属病院, 助教 (70464328)
• Project Period (FY): 2009 – 2011
• Keywords: メラトニン / 活性酸素 / 卵子 / 顆粒膜細胞 / 酸化ストレス

Research Abstract

Reactive oxygen species(ROS) are produced within the fbllicle especially during the ovulatory process. We examined whether melatonin acts as an anti-oxidant within follicles and protects oocytes and granulosa cells from oxidative stress. Melatonin, secreted by the pineal gland, is taken up into thefollicular fluid from the blood. ROS produced within the follicles, especially during the ovulation process, were scavenged by melatonin, and reduced oxidative stress may be involved in oocyte maturation, embryo development and luteinaization of granulosa cells. Our clinical study demonstrated that melatonin treatment for infertile women increases intra-fbllicular melatonin concentrations, reduces intra-follicular oxidative damage and elevates fertilization and pregnancy rates. Melatonin treatment also improves progesterone production by corpus luteum in infertile women with luteal phase defect.

Research Products

• [Journal Article] The role of melatonin as an antioxidant in the fbllicle (2012)
  • Author(s): Tamura H, Takasaki A, Taketani T, Tanabe M, Kizuka F, Lee L, Tamura I, Maekawa R, Asada H, Yamagata Y, Sugino
  • Journal Title: N. J Ov Res Volume: (in press)
  • Peer Reviewed
• [Journal Article] Protective role ofmelatonin in progesterone production by human luteal cells (2011)
  • Author(s): Taketani T, Tamura H, Takasaki A, Lee L, Kizuka F, Tamura I, Taniguchi K, Maekawa R, Asada H, Shimamura K, Reiter RJ
  • Journal Title: Sugino N. J Pineal Res Volume: 51 Pages: 207-13
  • Peer Reviewed
• [Journal Article] Differential effects of progesterone on COX-2 and Mn-SOD expressions are associated with histone acetylation status ofthe promoter region in human endometrial stromal cells (2011)
  • Author(s): Tamura I, Taketani T, Lee L, Kizuka F, Taniguchi K, Maekawa R, Asada H, Tamura H
  • Journal Title: Sugino N. J Clin Endocrinol Metab Volume: 96 Pages: 1073-82
  • Peer Reviewed
• [Journal Article] Luteal blood flow in patients undergoing GnRH agonist long protocol (2011)
  • Author(s): Takasaki A, Tamura I, Kizuka F, Lee L, Maekawa R, Asada H, Taketani T, Tamura H, Shimamura K, Morioka H, Sugino N
  • Journal Title: J Ovarian Res Volume: 4 Pages: 2
  • Peer Reviewed
• [Journal Article] 生殖機能調節の新しい視点10.メラトニンの卵巣機能調節 (2010)
  • Author(s): 田村博史、杉野法広
  • Journal Title: 産科と婦人科 Volume: 第77巻3号 Pages: 310-316
• [Journal Article] Prostaglandin F2α(PGF2α) stimulates PTGS2 expression and PGF2αsynthesis through NFKB activation via reactive oxygen species in the corpus luteum of pseudopregnant rats (2010)
  • Author(s): Sugino N. Reproduction.
  • Journal Title: Sugino N. Reproduction Volume: 140(6) Pages: 885-892
  • Peer Reviewed
• [Journal Article] Progesterone increases manganese superoxide dismutase expression via a cAMP-dependent signaling mediated by noncanonical Wnt5a pathway in human endometrial stromal cells (2010)
  • Author(s): Matsuoka A, Kizuka F, Lee L, Tamura I, Taniguchi K, Asada H, Taketani T, Tamura H
  • Journal Title: Sugino N. J Clin Endocrinol Metab Volume: 95 Pages: 291-299
  • Peer Reviewed
• [Journal Article] 卵過程におけるメラトニンの防御的役割-メラトニンは卵胞内で卵と顆粒膜細胞を酸化ストレスから護る- (2009)
  • Author(s): 田村博史
  • Journal Title: 日本産科婦人科学会誌 Volume: 第61巻第10号 Pages: 1925-1934
• [Journal Article] Melatonin and the ovary ; physiological and pathophysiological implications.[ Review article] (2009)
  • Author(s): Tamura H, Nakamura Y, Korkmaz A, Manchester LC, Tan DX, Norihiro S
  • Journal Title: Reiter RJ. Fertil Steril Volume: 92 Pages: 328-343
  • Peer Reviewed
• [Journal Article] Combination of melatonin and a peroxisome proliferator-activated receptor-gamma agonist induces apoptosis in a breast cancer cell line (2009)
  • Author(s): Korkmaz A, Tamura H, Manchester LC, Ogden GB, Tan DX
  • Journal Title: Reiter RJ. J Pineal Res Volume: 46 Pages: 115-116
  • Peer Reviewed
• [Presentation] -メラトニン研究の最近の進歩と医療への応用-パネルディスカッション『私のメラトニン使用経験から』 (2011)
  • Author(s): 田村博史
  • Organizer: 第3回抗加齢内分泌研究会学術集会
  • Place of Presentation: 横浜市鶴見大学会館
  • Year and Date: 2011-09-04
• [Presentation] 松果体ホルモンであるメラトニンは卵胞内で卵や穎粒膜細胞を護るシンポジウム9「生殖機能の中枢性調節:最近の知見」 (2011)
  • Author(s): 田村博史
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 神戸市神戸国際会議場
  • Year and Date: 2011-04-23
• [Presentation] Influence of oxidative stress on oocyte quality and protective role of melatonin as an antioxidant (2010)
  • Author(s): Hiroshi Tamura, Toshiaki Taketani, Akihisa Takasaki, Norihiro Sugino
  • Organizer: IFFS 2010 20^<th> World Congress on Fertility and Sterility
  • Place of Presentation: Munich, Germany
  • Year and Date: 20100912-16
• [Presentation] Melatonin protects oocyte and granulosa cells from reactive oxygen species(ROS) during the ovulatory process (2009)
  • Author(s): Hiroshi Tamura, Toshiaki Taketani, Norihiro Sugino
  • Organizer: XI Congress of the European Biological Rhythms Society
  • Place of Presentation: Strasbourg, France
  • Year and Date: 20090822-28
• [Presentation] 松果体ホルモンメラトニンと生殖 (2009)
  • Author(s): 田村博史
  • Organizer: 第27回内分泌代謝学サマーセミナー
  • Place of Presentation: 福井県三国観光ホテル
  • Year and Date: 2009-07-16
• [Presentation] 松果体ホルモンメラトニンと生殖 (2009)
  • Author(s): 田村博史
  • Organizer: 第82回日本内分泌学会研究奨励賞受賞講演
  • Place of Presentation: 前橋市群馬県民会館
  • Year and Date: 2009-04-25
• [Presentation] 排卵過程におけるメラトニンの防御的役割-メラトニンは卵胞内で卵と顆粒膜細胞を酸化ストレスから護る- (2009)
  • Author(s): 田村博史
  • Organizer: 第61回日本産科婦人科学会学術講演会シンポジウム2『中枢神経関連生理活性物質の生殖機能へのかかわり』
  • Place of Presentation: 京都市国立京都国際会館
  • Year and Date: 2009-04-04