2011 Fiscal Year Final Research Report
Promoter and inhibitor factors of bone destruction in human middle ear cholesteatoma
| Project/Area Number |
21592168
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA K Atuhisa 順天堂大学, 医学部, 教授 (70159614)
HURUKAWA Masayuki 順天堂大学, 医学部, 准教授 (20359524)
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| Project Period (FY) |
2009 – 2011
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| Keywords | ヒト中耳真珠腫 / カテプシン / SOD / RANKL / IL-17 |
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| Research Abstract |
Introduction : It has been reported that cathepsin L(as one of cystein proteinases) powerfully degrade type I collagen leading to the bone destruction and receptor activator of NF-Kapper B ligand (RANKL) is an essential factor for osteoclast differentiation, furthermore, osteoclasts secrete alarge mount of cathepsin L. IL-17A can induce RANKL. Therefore, we studied cathepsin L and RANKL in human middle ear cholesteatoma. Moreover, Cu, Zn-containing superoxide dismutase(Cu, Zn-SOD) as inhibitors of cathepsin L, IL17A or RANKL were observed. From above data, It was discussed between epithelia and subepithelial granulations.
Subject and Method : The human middle ear tissues evaluated in this study were surgically obtained frompatients with cholesteatoma. Expression of specimens was Cathepsin L, RANKL and Cu, Zn-SOD were examined immunohistochemically.
Results : The degree of bone destruction was dependent on the number of IL-17 positive cells. It was a significant correlation between Il-17 and RANKL positive cells. RANKL and Il-17 immunoreactivity was detected in subepithelial granulation tissues than in epithelia. Reversely, Cu, Zn-SOD with inhibitors of RANKL and IL-17 was more strongly positive in epithelia than in subepithelial granulation tissues.
These results suggest that a field related to the bone destruction in human cholesteatoma is subepithelial granulations rather than epithelia.
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