Identification of differentially expressed microRNAs in head and neck squamous cell carcinoma based on microRNA expression signature

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592187
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Chiba University
• Principal Investigator: SEKI Naohiko 千葉大学, 大学院・医学研究院, 准教授 (50345013)
• Co-Investigator(Kenkyū-buntansha): HANAZAWA Toyoyuki 千葉大学, 大学院・医学研究院, 准教授 (90272327)
• Project Period (FY): 2009 – 2011
• Keywords: 癌 / マイクロRNA / 機能性核酸 / 癌抑制遺伝子 / 頭頸部扁平上皮癌

Research Abstract

MicroRNAs (miRNAs) are endogenous short non-coding RNA molecules that regulate gene expression by repressing translation or cleaving RNA transcripts in a sequence-specific manner. A growing body of evidence suggests that miRNAs are aberrantly expressed in many human cancers and that they play significant roles in the initiation, development and metastasis of human cancers. Recently, our expression profile revealed that microRNA-1, microRNA-133a and microRNA-375 are frequently downregulated in head and neck squamous cell carcinoma (HNSCC). Ectopic expression of three miRNAs (microRNA-1, microRNA-133a and microRNA-375) inhibited cell growth, migration and invasion of HNSCC cells, suggesting these microRNAs function as tumor suppressors in HNSCC.

Research Products

• [Journal Article] Tumour suppressive microRNA-874 regulates novel cancer networks in maxillary sinus squamous cell carcinoma (2011)
  • Author(s): Nohata N, Hanazawa T, Kikkawa N, Sakurai D, Fujimura L, Chiyomaru T, Kawakami K, Yoshino H, Enokida H, Nakagawa M, Katayama A, Harabuchi Y, Okamoto Y, Seki N
  • Journal Title: Br J Cancer Volume: 105 Pages: 833-41
  • Peer Reviewed
• [Journal Article] Tumor suppressive microRNA-375 regulates oncogene AEG-1/MTDH in head and neck squamous cell carcinoma (HNSCC) (2011)
  • Author(s): Nohata N, Hanazawa T, Kikkawa N, Mutallip M, Sakurai D, Fujimura L, Kawakami K, Chiyomaru T, Yoshino H, Enokida H, Nakagawa M, Okamoto Y, Seki N
  • Journal Title: J Hum Genet Volume: 56 Pages: 595-601
  • Peer Reviewed
• [Journal Article] Identification of novel molecular targets regulated by tumor suppressive miR-1/miR-133a in maxillary sinus squamous cell carcinoma (2011)
  • Author(s): Nohata N, Hanazawa T, Kikkawa N, Sakurai D, Sasaki K, Chiyomaru T, Kawakami K, Yoshino H, Enokida H, Nakagawa M, Okamoto Y, Seki N
  • Journal Title: Int J Oncol Volume: 39 Pages: 1099-107
  • Peer Reviewed
• [Journal Article] miR-1 as a tumor suppressive microRNA targeting TAGLN2 in head and neck squamous cell carcinoma (2011)
  • Author(s): Nohata N, Sone Y, Hanazawa T, Fuse M, Kikkawa N, Yoshino H, Chiyomaru T, Kawakami K, Enokida H, Nakagawa M, Shozu M, Okamoto Y, Seki N
  • Journal Title: Oncotarget Volume: 2 Pages: 29-42
  • Peer Reviewed
• [Journal Article] Caveolin-1 mediates tumor cell migration and invasion and its regulation by miR-133a in head and neck squamous cell carcinoma (2011)
  • Author(s): Nohata N, Hanazawa T, Kikkawa N, Mutallip M, Fujimura L, Yoshino H, Kawakami K, Chiyomaru T, Enokida H, Nakagawa M, Okamoto Y, Seki N
  • Journal Title: Int J Oncol Volume: 38 Pages: 209-17
  • Peer Reviewed
• [Journal Article] miR-489 is a tumour-suppressive miRNA target PTPN11 in hypopharyngeal squamous cell carcinoma (HSCC) (2010)
  • Author(s): Kikkawa N, Hanazawa T, Fujimura L, Nohata N, Suzuki H, Chazono H, Sakurai D, Horiguchi S, Okamoto Y, Seki N
  • Journal Title: Br J Cancer Volume: 103 Pages: 877-84
  • Peer Reviewed
• [Presentation] Tumor suppressive microRNA-1/-133a regulates multiple oncogenes in maxillary sinus squamous cell carcinoma (2011)
  • Author(s): Nijiro Nohata, Toyoyuki Hanazawa, Takashi Kinoshita, Naoko Kikkawa, Muradil Mutallip, Takeshi Chiyomaru, Kazumori Kawakami, Hirofumi Yoshino, Hideki Enokida, Masayuki Nakagawa, Yoshitaka Okamoto, Naohiko Seki
  • Organizer: 16th World Congress on Advances in Oncology and 14th International Symposium on Molecular Medicine
  • Place of Presentation: Rhodes Island, Greece
  • Year and Date: 20111006-08
• [Presentation] Tumor suppressive microRNA-375 targeting multiple oncogenes in maxillary sinus squamous cell carcinoma (MSSCC) (2011)
  • Author(s): Takashi Kinoshita, Nijiro Nohata, Toyoyuki Hanazawa, Naoko Kikkawa, Mutallip Muradil, Hirofumi Yoshino, Kazumori Kawakami, Takeshi Chiyomaru, Hideki Enokida, Masayuki Nakagawa, Yoshitaka Okamoto, Naohiko Seki
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場名古屋
  • Year and Date: 20111003-0405
• [Presentation] miR-133a as a tumor suppressive microRNA targeting multiple oncogenes in head neck squamous cell carcinoma (2011)
  • Author(s): Nijiro Nohata, Toyoyuki Hanazawa, Naoko Kikkawa, Miki Fuse, Takeshi Chiyomaru, Kazumori Kawakami, Hideki Enokida, Masayuki Nakagawa, Yoshitaka Okamoto, Naohiko Seki
  • Organizer: AACR 102nd Annual Meeting
  • Place of Presentation: Orlando, Florida
  • Year and Date: 20110412-06
• [Presentation] Identification of tumor suppressive micro RNAs in maxillary sinus squamous cell carcinoma based on micro RNA expression signature (2011)
  • Author(s): Toyoyuki Hanazawa, Nijiro Nohata, Naoko Kikkawa, Akihiro Katayama, Yasuaki Harabuchi, Yoshitaka Okamoto, Naohiko Seki
  • Organizer: AACR 102nd Annual Meeting
  • Place of Presentation: Orlando, Florida
  • Year and Date: 20110412-06
• [Remarks]
  • URL: http://genomejet.jp