Investigation of epigenetic factors in the pathogenesis of glucocorticoid-induced glaucoma

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592239
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Juntendo University
• Principal Investigator: MATSUDA Akira 順天堂大学, 医学研究科, 准教授 (00312348)
• Co-Investigator(Kenkyū-buntansha): KAWASAKI Satoshi 京都府立医科大学, 医学研究科, 助教 (60347458) ; MORI Kakuhiko 京都府立医科大学, 医学研究科, 講師 (40252001)
• Co-Investigator(Renkei-kenkyūsha): TASHIRO Kei 京都府立医科大学, 医学研究科, 教授 (10263097) ; IKEDA Youko 京都府立医科大学, 医学研究科, 助教 (00433243) ; KINOSHITA Shigeru 京都府立医科大学, 医学研究科, 教授 (30116024) ; MARUYAMA Kazuichi 京都府立医科大学, 医学研究科, 助教 (10433244)
• Project Period (FY): 2009 – 2011
• Keywords: エピジェネティクス / 緑内障 / ステロイド / ゲノム / 遺伝子多型

Research Abstract

The effects for dexamethasone(DEX) treatment for the genomic methylation status of human trabecular meshwork cells were analyzed by whole genome methylation array analysis. DEX treatment changed the expression level of 23 genes significantly. We analyzed the functional effect of genomic methylation/demethylation of RICS, RGS6 and GPR126 and found that the change of methylation status did affect not only the quantitative expression level but also qualitative expression pattern like the change of mRNA splicing.

Research Products

• [Journal Article] Lymphoid neogenesis in the giant papillae of patients with chronic allergic conjunctivitis (2010)
  • Author(s): Matsuda A, Ebihara N, Yokoi N, Maruyama K, Hamuro J, Kinoshita S, Murakami A.
  • Journal Title: J Allergy Clin Immunol Volume: 126 Pages: 1310-1312
  • Peer Reviewed
• [Journal Article] Basophils in the giant papillae of chronic allergic keratoconjunctivitis (2010)
  • Author(s): Matsuda A, Ebihara N, Yokoi N, Okayama Y, Watanabe Y, Kawasaki S, Tanioka H, Walls AF, Hamuro J, Kinoshita S, Murakami A.
  • Journal Title: Br J Ophthalmol Volume: 94 Pages: 513-518
  • Peer Reviewed
• [Journal Article] Murakami A. Functional Roles of Thymic Stromal Lymphopoietin for Chronic Allergic Keratoconjunctivitis (2010)
  • Author(s): Matsuda A, Ebihara N, Yokoi N, Kawasaki S, Tanioka H, Inatomi T, de Waal Malefyt R, Hamuro J, Kinoshita S
  • Journal Title: Invest Ophthalmol Volume: 51 Pages: 151-155
  • Peer Reviewed
• [Journal Article] Three susceptible loci associated with primary-open angle glaucoma identified by genome-wide association study in Japanese population (2009)
  • Author(s): Nakano M, Ikeda Y, Taniguchi T, Yagi T, Fuwa M, Omi N, Tokuda Y, Tanaka M, Yoshii K, Kageyama M, Naruse S, Matsuda A, Mori K, Kinoshita S, Tashiro K.
  • Journal Title: Proc Natl Acad Volume: 106 Pages: 12838-12842
  • Peer Reviewed
• [Journal Article] The Role of Interleukin-33 in Chronic Allergic Conjunctivitis (2009)
  • Author(s): Matsuda A, Okayama Y, Terai N, Yokoi N, Ebihara N, Tanioka H, Kawasaki S, Inatomi T, Katoh N, Ueda E, Hamuro J, Murakami A, Kinoshita S.
  • Journal Title: Invest Ophthalmol Volume: 50 Pages: 4646-4652
  • Peer Reviewed
• [Journal Article] Involvement of Plasminogen Activator Inhibitor-1 in the Pathogenesis of Atopic Cataracts
  • Author(s): Hori K, Matsuda A, Ebihara N, Imai K, Mori K, Funaki T, Watanabe Y, Nakatani S, Okada K, Matsuo O, Murakami A.
  • Journal Title: Invest Ophthalmol Volume: (in Press)
  • Peer Reviewed
• [Journal Article] Perlecan-Deficient Mutation Impairs Corneal Epithelial Structure
  • Author(s): Inomata T, Ebihara N, Funaki T, Matsuda A, Watanabe Y, Ning L, Xu Z, Murakami A, Arikawa-Hirasawa A.
  • Journal Title: Invset Ophthalmol Volume: (in Press)
  • Peer Reviewed
• [Journal Article] Role of the IL-6 classic and trans-signaling pathways in corneal sterile inflammation and wound healing
  • Author(s): Ebihara N, Matsuda A, Nakamura S, Matsuda H and Murakami A.
  • Journal Title: Invset Ophthalmol Volume: (in Press)
  • Peer Reviewed
• [Presentation] デキサメサゾンによるヒト培養線維柱帯細胞のゲノムメチル化状態の変化 (2010)
  • Author(s): 松田彰、海老原伸行、村上晶
  • Organizer: 第21回日本緑内障学会
  • Place of Presentation: 福岡
  • Year and Date: 2010-09-24
• [Presentation] Th2サイトカイン環境は結膜繊維芽細胞のコラーゲンゲル収縮能を増加させる (2009)
  • Author(s): 松田彰、海老原伸行、村上晶
  • Organizer: 第20回日本緑内障学会
  • Place of Presentation: 沖縄
  • Year and Date: 2009-11-14