2011 Fiscal Year Final Research Report
Effect of PACAP on adult retinal stem cell and the regenerative potential.
| Project/Area Number |
21592246
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Showa University |
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Principal Investigator |
SEKI Tamotsu 昭和大学, 医学部, 兼任講師 (10245855)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMACHI Tomoya 昭和大学, 遺伝子組換え実験室, 助教 (30433840)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIODA Seiji 昭和大学, 医学部, 教授 (80102375)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 眼組織学 / 緑内障 / PACAP / 網膜保護 |
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| Research Abstract |
Retinal neuroprotective effect of PACAP was evaluated in transient ischemia model induced by high intraocular pressure. PACAP suppressed retinal ganglion cells(RGCs) with bimodal fashion with cAMP and MAPK pathways. Retinal protective effect of endogenous PACAP was examined using heterozygous PACAP KO mice with NMDA model. heterozygous PACAP KO mice showed high number of RGCs and less number of TUNEL positive cells in NMDA-treated retina. These data suggest that exogenous and endogenous PACAP have a neuroprotective potential in retinal RGCs. Furthermore, number of BrdU positive cells peaked on day 3 after NMDA treatment, and about 90% of BrdU positive cells were overlapped with microglia/macrophage marker. PACAP administration increased the number of BrdU positive cells after NMDA-treatment. Increment of the number of BrdU positive cells may be involved in neuroprotective effect of PACAP.
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Research Products
(16 results)
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[Journal Article] Neuroprotective effect of PACAP against NMDA-induced retinal damage in the mouse2011
Author(s)
Endo K, Nakamachi T, Seki T, Kagami N, Wada Y, Nakamura K, Kishimoto K, Hori M, Tsuchikawa D, Shinntani N, Hashimoto H, Baba A, Koide R, Shioda S
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Journal Title
J Mol Neurosci
Volume: 43(1)
Pages: 22-29
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[Presentation] PACAP STIMULATES THE LACRIMATION VIA AQUAPORIN 5 TRANSLOCATOIN IN MOUSE2011
Author(s)
Nakamachi T, Aizawa Y, Ohtaki H, Seki T, Wada Y, Kagami N, Arata S, Hashimoto H, Shintani N, Baba A, Farkas J, Reglodi D and Shioda S
Organizer
The 10th international Symposium on VIP, PACAP and Related Peptides
Place of Presentation
Eilat, Israel
Year and Date
20111200
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[Presentation] Effect of pituitary adenylate cyclase-activating polypeptide(PACAP) on reactive astrocyte induced by brain damage in mice2011
Author(s)
Tomoya Nakamachi, Keisuke Nakamura, Yoshihiro Wada, Nobuyuki Kagami, Daisuke Tsuchikawa, Motohide Hori, Nori Imai, Akira Yoshikawa, Tamotsu Seki, Satoru Arata, and Seiji Shioda
Organizer
The 41th Annual Meeting of the Society for Neuroscience
Place of Presentation
Washington, DC
Year and Date
20111100
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[Presentation] Effect of PACAP on tear secretion in mouse2009
Author(s)
Nakamachi T, Aizawa Y, Ohtaki H, Yofu S, Seki T, Endo K, Nakamura K, Kagami N, Arata S, Shintani N, Hashimoto H, Baba A, Shioda S
Organizer
The 9th International Symposium on VIP, PACAP and Related Peptides
Place of Presentation
Kagoshima
Year and Date
20091000
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